ISAde4

  • Family IS3
  • Group IS407
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_011891 ND Anaeromyxobacter dehalogenans
Anaeromyxobacter dehalogenans 2CP-1
DNA section
IS Length : 1183 bp

Ends


IR Length : 32/42

IRL : TGACCTGCCCCCCGAAAACTGGACGGATCTGACTGCCAGTCATCTACTGA
IRR : TGACCTGCCCCGCATCACTGGGCGGCTGATGATGCCAGTCCGGTGAGACT

Insertion site


Left flankDirect repeatRight flankDR Length
CGCGCGTTCGAAGCGACTGAGATG4

DNA sequence

TGACCTGCCCCCCGAAAACTGGACGGATCTGACTGCCAGTCATCTACTGACAGTGGAGGACGGATGCGGAAGAGCAAGTTCACCGAAGAGCAGATCATCA
AGGTGCTGAACGAGGTCGAGGCCGGTGCCAAGGTCCAGGACGTGACCCGGAAGGTCGGGATCACCGAGACGACCTTCTACCGGTGGAAGTCGAAGTACGG
CGGGCTGGAGGTCTCGGACGTGAAGCGCCTCAAGGCGCTCGAGGATGAGAACCGGAAGCTCAAAACCCTGGTCGCCGATCTCACCCTCGACAAGCAGGCG
CTGCAGCACCTTCTCGGAAAAAAATGGTAGGGCCCGCCGCGCTTCGCCCGGCGGTAATCGAGCTGGGGGCGAAGTTCGCGATGAAGAAGCGTCGGGCCTG
TCGCGTCGTCGGGCTCGCGCCGTCGACGCTCTACTACTGCAGCCGCCGGCCGGAGCGCGCCGAAGTCCGGGCACGCCTGCGGGATCTAGCCGCCCAGCGT
CCGCGCTGGGGCTACCGGCGCCTGCATGTGCTCCTCGACCGCGAAGGGCACCACCTGAACCACAAGCTCGTGTTCCGGCTGTACCGCTCGGAGGGGCTGG
CTGTCAGGCGAAAGAGGCGGAAGCGGATCACGTCGTCACTGCGCGTGGTTCCACCGCCGCCGACGCGCCCGCGCCAGCAGTGGACGATGGACTTCACGCA
GGACTCGCTCGCAAGCGGACGCCAGTTCCGGACCCTGAACTTGATCGACGCCTTCACCCGCGAGTGCCTGCTCATCGAGGCCGACCACTCGCTCACCGGG
GCGCGCGTCGTGCGCGCGCTGGAGCGCCTCCGCGAGCTCCACGGCACGCCCGAGGTCATCCGCATCGACAACGGGACGGAGTTCACGAGCAGCGCGGTGG
ACGCCTGGGCCTATACCAATCAGGTCCGCCTGGACTTCATCACGCCGGGCAAGCCGACCGAGAACGGGCACATCGAGAGCTTCAACGGGAAGTTCCGCGA
CGAGTGCCTGAACGAGAACTGGTTCATCAGCCTCGATGACGTCCGTCGCAAGGTCGAGGCGTATCGAGTCGACTACAACGAGGTCAGGCCGCACAGTTCG
CTGGACAACAGGACGCCAAACGAGTTGGCTCACAGTCTCACCGGACTGGCATCATCAGCCGCCCAGTGATGCGGGGCAGGTCA
Recoding section
  • Recoding by frameshift
  • Frame
  • Type
  • Experimentally demonstrated

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
267 bp88 aa64330+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MRKSKFTEEQIIKVLNEVEAGAKVQDVTRKVGITETTFYRWKSKYGGLEVSDVKRLKALEDENRKLKTLVADLTLDKQALQHLLGKKW

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
921 bp306 aa2491169+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

EPEAQNPGRRSHPRQAGAAAPSRKKMVGPAALRPAVIELGAKFAMKKRRACRVVGLAPSTLYYCSRRPERAEVRARLRDLAAQRPRWGYRRLHVLLDREG
HHLNHKLVFRLYRSEGLAVRRKRRKRITSSLRVVPPPPTRPRQQWTMDFTQDSLASGRQFRTLNLIDAFTRECLLIEADHSLTGARVVRALERLRELHGT
PEVIRIDNGTEFTSSAVDAWAYTNQVRLDFITPGKPTENGHIESFNGKFRDECLNENWFISLDDVRRKVEAYRVDYNEVRPHSSLDNRTPNELAHSLTGL
ASSAAQ

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1106 bp368 aa641169+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MRKSKFTEEQIIKVLNEVEAGAKVQDVTRKVGITETTFYRWKSKYGGLEVSDVKRLKALEDENRKLKTLVADLTLDKQALQHLLGKKMVGPAALRPAVIE
LGAKFAMKKRRACRVVGLAPSTLYYCSRRPERAEVRARLRDLAAQRPRWGYRRLHVLLDREGHHLNHKLVFRLYRSEGLAVRRKRRKRITSSLRVVPPPP
TRPRQQWTMDFTQDSLASGRQFRTLNLIDAFTRECLLIEADHSLTGARVVRALERLRELHGTPEVIRIDNGTEFTSSAVDAWAYTNQVRLDFITPGKPTE
NGHIESFNGKFRDECLNENWFISLDDVRRKVEAYRVDYNEVRPHSSLDNRTPNELAHSLTGLASSAAQ

 

Blast result :
Comments
ISAde4 is 70% aa similar to ISDet2. The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] ISfinder annotation (2009)
2] Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Saunders,E., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Ovchinnikova,G., Beliaev,A.S. and Richardson,P. (2009) Direct submission GenBank.