ISAde4
- Family IS3
- Group IS407
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_011891 | ND | Anaeromyxobacter dehalogenans | Anaeromyxobacter dehalogenans 2CP-1 |
DNA section
IS Length : 1183 bp
Ends
IR Length : 32/42
IRL : TGACCTGCCCCCCGAAAACTGGACGGATCTGACTGCCAGTCATCTACTGA
IRR : TGACCTGCCCCGCATCACTGGGCGGCTGATGATGCCAGTCCGGTGAGACT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CGCGCGTTCG | AAGC | GACTGAGATG | 4 |
DNA sequence
TGACCTGCCCCCCGAAAACTGGACGGATCTGACTGCCAGTCATCTACTGACAGTGGAGGACGGATGCGGAAGAGCAAGTTCACCGAAGAGCAGATCATCA
AGGTGCTGAACGAGGTCGAGGCCGGTGCCAAGGTCCAGGACGTGACCCGGAAGGTCGGGATCACCGAGACGACCTTCTACCGGTGGAAGTCGAAGTACGG
CGGGCTGGAGGTCTCGGACGTGAAGCGCCTCAAGGCGCTCGAGGATGAGAACCGGAAGCTCAAAACCCTGGTCGCCGATCTCACCCTCGACAAGCAGGCG
CTGCAGCACCTTCTCGGAAAAAAATGGTAGGGCCCGCCGCGCTTCGCCCGGCGGTAATCGAGCTGGGGGCGAAGTTCGCGATGAAGAAGCGTCGGGCCTG
TCGCGTCGTCGGGCTCGCGCCGTCGACGCTCTACTACTGCAGCCGCCGGCCGGAGCGCGCCGAAGTCCGGGCACGCCTGCGGGATCTAGCCGCCCAGCGT
CCGCGCTGGGGCTACCGGCGCCTGCATGTGCTCCTCGACCGCGAAGGGCACCACCTGAACCACAAGCTCGTGTTCCGGCTGTACCGCTCGGAGGGGCTGG
CTGTCAGGCGAAAGAGGCGGAAGCGGATCACGTCGTCACTGCGCGTGGTTCCACCGCCGCCGACGCGCCCGCGCCAGCAGTGGACGATGGACTTCACGCA
GGACTCGCTCGCAAGCGGACGCCAGTTCCGGACCCTGAACTTGATCGACGCCTTCACCCGCGAGTGCCTGCTCATCGAGGCCGACCACTCGCTCACCGGG
GCGCGCGTCGTGCGCGCGCTGGAGCGCCTCCGCGAGCTCCACGGCACGCCCGAGGTCATCCGCATCGACAACGGGACGGAGTTCACGAGCAGCGCGGTGG
ACGCCTGGGCCTATACCAATCAGGTCCGCCTGGACTTCATCACGCCGGGCAAGCCGACCGAGAACGGGCACATCGAGAGCTTCAACGGGAAGTTCCGCGA
CGAGTGCCTGAACGAGAACTGGTTCATCAGCCTCGATGACGTCCGTCGCAAGGTCGAGGCGTATCGAGTCGACTACAACGAGGTCAGGCCGCACAGTTCG
CTGGACAACAGGACGCCAAACGAGTTGGCTCACAGTCTCACCGGACTGGCATCATCAGCCGCCCAGTGATGCGGGGCAGGTCA
AGGTGCTGAACGAGGTCGAGGCCGGTGCCAAGGTCCAGGACGTGACCCGGAAGGTCGGGATCACCGAGACGACCTTCTACCGGTGGAAGTCGAAGTACGG
CGGGCTGGAGGTCTCGGACGTGAAGCGCCTCAAGGCGCTCGAGGATGAGAACCGGAAGCTCAAAACCCTGGTCGCCGATCTCACCCTCGACAAGCAGGCG
CTGCAGCACCTTCTCGGAAAAAAATGGTAGGGCCCGCCGCGCTTCGCCCGGCGGTAATCGAGCTGGGGGCGAAGTTCGCGATGAAGAAGCGTCGGGCCTG
TCGCGTCGTCGGGCTCGCGCCGTCGACGCTCTACTACTGCAGCCGCCGGCCGGAGCGCGCCGAAGTCCGGGCACGCCTGCGGGATCTAGCCGCCCAGCGT
CCGCGCTGGGGCTACCGGCGCCTGCATGTGCTCCTCGACCGCGAAGGGCACCACCTGAACCACAAGCTCGTGTTCCGGCTGTACCGCTCGGAGGGGCTGG
CTGTCAGGCGAAAGAGGCGGAAGCGGATCACGTCGTCACTGCGCGTGGTTCCACCGCCGCCGACGCGCCCGCGCCAGCAGTGGACGATGGACTTCACGCA
GGACTCGCTCGCAAGCGGACGCCAGTTCCGGACCCTGAACTTGATCGACGCCTTCACCCGCGAGTGCCTGCTCATCGAGGCCGACCACTCGCTCACCGGG
GCGCGCGTCGTGCGCGCGCTGGAGCGCCTCCGCGAGCTCCACGGCACGCCCGAGGTCATCCGCATCGACAACGGGACGGAGTTCACGAGCAGCGCGGTGG
ACGCCTGGGCCTATACCAATCAGGTCCGCCTGGACTTCATCACGCCGGGCAAGCCGACCGAGAACGGGCACATCGAGAGCTTCAACGGGAAGTTCCGCGA
CGAGTGCCTGAACGAGAACTGGTTCATCAGCCTCGATGACGTCCGTCGCAAGGTCGAGGCGTATCGAGTCGACTACAACGAGGTCAGGCCGCACAGTTCG
CTGGACAACAGGACGCCAAACGAGTTGGCTCACAGTCTCACCGGACTGGCATCATCAGCCGCCCAGTGATGCGGGGCAGGTCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 267 bp | 88 aa | 64 | 330 | + | No |
Description : First part of the transposase
ORF sequence :
MRKSKFTEEQIIKVLNEVEAGAKVQDVTRKVGITETTFYRWKSKYGGLEVSDVKRLKALEDENRKLKTLVADLTLDKQALQHLLGKKW
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 921 bp | 306 aa | 249 | 1169 | + | No |
Description : Second part of the transposase
ORF sequence :
EPEAQNPGRRSHPRQAGAAAPSRKKMVGPAALRPAVIELGAKFAMKKRRACRVVGLAPSTLYYCSRRPERAEVRARLRDLAAQRPRWGYRRLHVLLDREG
HHLNHKLVFRLYRSEGLAVRRKRRKRITSSLRVVPPPPTRPRQQWTMDFTQDSLASGRQFRTLNLIDAFTRECLLIEADHSLTGARVVRALERLRELHGT
PEVIRIDNGTEFTSSAVDAWAYTNQVRLDFITPGKPTENGHIESFNGKFRDECLNENWFISLDDVRRKVEAYRVDYNEVRPHSSLDNRTPNELAHSLTGL
ASSAAQ
HHLNHKLVFRLYRSEGLAVRRKRRKRITSSLRVVPPPPTRPRQQWTMDFTQDSLASGRQFRTLNLIDAFTRECLLIEADHSLTGARVVRALERLRELHGT
PEVIRIDNGTEFTSSAVDAWAYTNQVRLDFITPGKPTENGHIESFNGKFRDECLNENWFISLDDVRRKVEAYRVDYNEVRPHSSLDNRTPNELAHSLTGL
ASSAAQ
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1106 bp | 368 aa | 64 | 1169 | + | Yes |
Chemistry : DDE
ORF sequence :
MRKSKFTEEQIIKVLNEVEAGAKVQDVTRKVGITETTFYRWKSKYGGLEVSDVKRLKALEDENRKLKTLVADLTLDKQALQHLLGKKMVGPAALRPAVIE
LGAKFAMKKRRACRVVGLAPSTLYYCSRRPERAEVRARLRDLAAQRPRWGYRRLHVLLDREGHHLNHKLVFRLYRSEGLAVRRKRRKRITSSLRVVPPPP
TRPRQQWTMDFTQDSLASGRQFRTLNLIDAFTRECLLIEADHSLTGARVVRALERLRELHGTPEVIRIDNGTEFTSSAVDAWAYTNQVRLDFITPGKPTE
NGHIESFNGKFRDECLNENWFISLDDVRRKVEAYRVDYNEVRPHSSLDNRTPNELAHSLTGLASSAAQ
LGAKFAMKKRRACRVVGLAPSTLYYCSRRPERAEVRARLRDLAAQRPRWGYRRLHVLLDREGHHLNHKLVFRLYRSEGLAVRRKRRKRITSSLRVVPPPP
TRPRQQWTMDFTQDSLASGRQFRTLNLIDAFTRECLLIEADHSLTGARVVRALERLRELHGTPEVIRIDNGTEFTSSAVDAWAYTNQVRLDFITPGKPTE
NGHIESFNGKFRDECLNENWFISLDDVRRKVEAYRVDYNEVRPHSSLDNRTPNELAHSLTGLASSAAQ
Blast result :
Comments
ISAde4 is 70% aa similar to ISDet2. The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] ISfinder annotation (2009)
2] Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Saunders,E., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Ovchinnikova,G., Beliaev,A.S. and Richardson,P. (2009) Direct submission GenBank.
2] Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Saunders,E., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Ovchinnikova,G., Beliaev,A.S. and Richardson,P. (2009) Direct submission GenBank.