ISAde2
- Family IS1182
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_011891 | ND | Anaeromyxobacter dehalogenans | Anaeromyxobacter dehalogenans 2CP-1 |
DNA section
IS Length : 1498 bp
Ends
IR Length : 14/17
IRL : GAGAGCGTGAAGGAATCGGCCGGTGCCCCATGGACTGGGGCCGCCGGCCG
IRR : GGACCGTGAAGTAATCCGTCCGCGCTGCGGGTGCGGCGCGGGCAGGCGCC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TGAGCGAGCG | CTGA | GCCGCCCCGA | 4 |
| CGCCGGGGCG | CTGA | GAGCACGTGA | 4 |
| CGGCGGTGCA | CTGA | GTGGACCCGC | 4 |
| TGGTCCGGCG | CTGA | GAGCACGTGA | 4 |
| TCAGCGTGCG | CTGA | GCCCGCGCCG | 4 |
| CGCCGGTGCA | CTGA | CAGCACGAGC | 4 |
| TGAAGGTGCG | CTGA | GCGGGCCCGG | 4 |
| GCAGCGCGCG | CTGA | GAGCACGTGA | 4 |
| TTCACGCGCT | CTGA | GAGCACGTGA | 4 |
DNA sequence
GAGAGCGTGAAGGAATCGGCCGGTGCCCCATGGACTGGGGCCGCCGGCCGTGATCTTCTCGGGGCGTGAGCGAACACGACGCGCCGCGGGTGGTGAGGCC
GGTCCGAAATCAACTCTCGCTGCAGCCGACGGACCTCGAAGCGCTGGTCCCGGACGAGCACCCGGTTCGCGCCATCTGGACGTTGGTCGAGCGGCTGGAC
CTGTCGGCGTTCTACGACGAGATCGCGTCGCGCGGCAGCAATGCTGGGCGGCCAGCAACGGACCCGGCGGTGCTGCTCGCGCTGTGGCTGTTCGCCAACT
CGGAGGGCGTGGGGAGCGCGCGGTTGCTCGAGCGGCTGTGCGAGCGGGACGCGCCCTACCGCTGGATCTGCGGCGGGGTGCCGGTGAACCACCATACGCT
CGCCGACTTTCGCGTGGAGCACGGGAAGAAGCTCGACCGGCTCATGACGCAGGTGCTGGCCGCGCTCATGAAGGAAGGCGTGGTGCAGCTCCGGCGCGTG
GCGCAGGACGGGATGAAGGTCCGGGCGAGCGCCGGCGCGGCGAGCTTCCGGCGTCGTCAGTCACTGGAGCGCTGCCGCAAAGAAGCCGAGGAACAGGTCC
GAAAGCTCAGGCGCGAGATCGAGAGCGATCCGTCAGCTTCGACGAAGCGTGTTCAGGCTGCGAAGGAGCGGGCCGCGCAAGCTCGGCTCGATGCCGTCGA
GGCCGCACTTGCGGAGGTCGAAGTCGTCGAGAAGCAGCGCGTCGAGCGAGACGAGAAGAAGCCCTCGGACGCGAGGCGTCGCGGCGAGATCCGCGTCAGC
ACCACGGACGCGGAGAGCCGCGTGATGAAGATGGCGGACGGTGGGTTCCGGCCCGCCTACAACGTGCAGTTCGCGACGGACGAGAGCGGTGTCATCGTGG
GCACCGACGTCACGAACAACGGTACCGACCAGCCGCACGTCGTGCCGATGCTCGACGAGATCCGGCGCCGAACGGGCGAGACGCCACGCGAGTACCTGGT
CGACGGTGGCTTCGTGACGCTCGACAACATCGAGGCCATCGCAGAGCGGGGCGCGACTCCGTATGCGCCGCTGCCGAAGCCGAAGAGCAAAGCCGTCGAT
CCGCACGCTCCGAAGCGCAACGACACACCTGCGATCGGGGCCTGGCGCGTGCGGATGGGGACAGAGGACGCCAAGCGCGTCTACGTGCAGCGCGGCGTCC
TCGCCGAGAGGACGAACGCAGATCTCCGTGTGCACCGGCGACTCGACCGGCTGAACGTCCGAGGGCTGGTAAAGGTGAAGACGATCGTGCTGCTCGCCGC
GATCAGCTTCAACATCATGCGTCTCGTCGCGAGCAGACTCTCGGCCTAATCGCCTCCGGACGGGACTCCCGGCGCCGGACCGAGCTCCGCCGGCTGAACG
AGCCCTCCGACGCCCCCGCGTCCCCGAAGGTGGGCGCGCTCGACCCCGGGCGCCTGCCCGCGCCGCACCCGCAGCGCGGACGGATTACTTCACGGTCC
GGTCCGAAATCAACTCTCGCTGCAGCCGACGGACCTCGAAGCGCTGGTCCCGGACGAGCACCCGGTTCGCGCCATCTGGACGTTGGTCGAGCGGCTGGAC
CTGTCGGCGTTCTACGACGAGATCGCGTCGCGCGGCAGCAATGCTGGGCGGCCAGCAACGGACCCGGCGGTGCTGCTCGCGCTGTGGCTGTTCGCCAACT
CGGAGGGCGTGGGGAGCGCGCGGTTGCTCGAGCGGCTGTGCGAGCGGGACGCGCCCTACCGCTGGATCTGCGGCGGGGTGCCGGTGAACCACCATACGCT
CGCCGACTTTCGCGTGGAGCACGGGAAGAAGCTCGACCGGCTCATGACGCAGGTGCTGGCCGCGCTCATGAAGGAAGGCGTGGTGCAGCTCCGGCGCGTG
GCGCAGGACGGGATGAAGGTCCGGGCGAGCGCCGGCGCGGCGAGCTTCCGGCGTCGTCAGTCACTGGAGCGCTGCCGCAAAGAAGCCGAGGAACAGGTCC
GAAAGCTCAGGCGCGAGATCGAGAGCGATCCGTCAGCTTCGACGAAGCGTGTTCAGGCTGCGAAGGAGCGGGCCGCGCAAGCTCGGCTCGATGCCGTCGA
GGCCGCACTTGCGGAGGTCGAAGTCGTCGAGAAGCAGCGCGTCGAGCGAGACGAGAAGAAGCCCTCGGACGCGAGGCGTCGCGGCGAGATCCGCGTCAGC
ACCACGGACGCGGAGAGCCGCGTGATGAAGATGGCGGACGGTGGGTTCCGGCCCGCCTACAACGTGCAGTTCGCGACGGACGAGAGCGGTGTCATCGTGG
GCACCGACGTCACGAACAACGGTACCGACCAGCCGCACGTCGTGCCGATGCTCGACGAGATCCGGCGCCGAACGGGCGAGACGCCACGCGAGTACCTGGT
CGACGGTGGCTTCGTGACGCTCGACAACATCGAGGCCATCGCAGAGCGGGGCGCGACTCCGTATGCGCCGCTGCCGAAGCCGAAGAGCAAAGCCGTCGAT
CCGCACGCTCCGAAGCGCAACGACACACCTGCGATCGGGGCCTGGCGCGTGCGGATGGGGACAGAGGACGCCAAGCGCGTCTACGTGCAGCGCGGCGTCC
TCGCCGAGAGGACGAACGCAGATCTCCGTGTGCACCGGCGACTCGACCGGCTGAACGTCCGAGGGCTGGTAAAGGTGAAGACGATCGTGCTGCTCGCCGC
GATCAGCTTCAACATCATGCGTCTCGTCGCGAGCAGACTCTCGGCCTAATCGCCTCCGGACGGGACTCCCGGCGCCGGACCGAGCTCCGCCGGCTGAACG
AGCCCTCCGACGCCCCCGCGTCCCCGAAGGTGGGCGCGCTCGACCCCGGGCGCCTGCCCGCGCCGCACCCGCAGCGCGGACGGATTACTTCACGGTCC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1284 bp | 427 aa | 66 | 1349 | + | No |
Chemistry : DDE
ORF sequence :
MSEHDAPRVVRPVRNQLSLQPTDLEALVPDEHPVRAIWTLVERLDLSAFYDEIASRGSNAGRPATDPAVLLALWLFANSEGVGSARLLERLCERDAPYRW
ICGGVPVNHHTLADFRVEHGKKLDRLMTQVLAALMKEGVVQLRRVAQDGMKVRASAGAASFRRRQSLERCRKEAEEQVRKLRREIESDPSASTKRVQAAK
ERAAQARLDAVEAALAEVEVVEKQRVERDEKKPSDARRRGEIRVSTTDAESRVMKMADGGFRPAYNVQFATDESGVIVGTDVTNNGTDQPHVVPMLDEIR
RRTGETPREYLVDGGFVTLDNIEAIAERGATPYAPLPKPKSKAVDPHAPKRNDTPAIGAWRVRMGTEDAKRVYVQRGVLAERTNADLRVHRRLDRLNVRG
LVKVKTIVLLAAISFNIMRLVASRLSA
ICGGVPVNHHTLADFRVEHGKKLDRLMTQVLAALMKEGVVQLRRVAQDGMKVRASAGAASFRRRQSLERCRKEAEEQVRKLRREIESDPSASTKRVQAAK
ERAAQARLDAVEAALAEVEVVEKQRVERDEKKPSDARRRGEIRVSTTDAESRVMKMADGGFRPAYNVQFATDESGVIVGTDVTNNGTDQPHVVPMLDEIR
RRTGETPREYLVDGGFVTLDNIEAIAERGATPYAPLPKPKSKAVDPHAPKRNDTPAIGAWRVRMGTEDAKRVYVQRGVLAERTNADLRVHRRLDRLNVRG
LVKVKTIVLLAAISFNIMRLVASRLSA
Blast result :
Comments
ISAde2 is 95% aa similar to ISAnsp1.
File updated: June 2 2010.
File updated: June 2 2010.
References
1] ISfinder annotation (2009)
2] Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Saunders,E., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Ovchinnikova,G., Beliaev,A.S. and Richardson,P. (2009) Direct submission GenBank.
2] Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Saunders,E., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Ovchinnikova,G., Beliaev,A.S. and Richardson,P. (2009) Direct submission GenBank.