ISfinder Mirror Site

ISAnsp12

Family IS66
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_009675	ND	Anaeromyxobacter sp.	Anaeromyxobacter sp. Fw109-5

DNA section

IS Length : 2926 bp

Ends

IR Length : 20/22

IRL : GTAACCGCCCGGGGATCGACGTCTGGCAGGGCCGACCGGCCGGGCTGATG
IRR : GTAACCGGCCGGTGATCGACGTGTTGCGGCCGGATTCGATGCGTCGATTC

Insertion site

Left flank	Direct repeat	Right flank	DR Length
CTTGCTGTGA	CGCGCTGG	CGCTCGCTGC	8

DNA sequence

GTAACCGCCCGGGGATCGACGTCTGGCAGGGCCGACCGGCCGGGCTGATGCTCCTCCGCTCACGACGGAGGACGGCATGGCCGAGCAGGATCGGACGCGG
TGGACGAAGCTTGTGGCGGACTTCGAGTCGAGCGACCTGACGCAGCGCGACTTCGCGACGGAGCGCGGGATCTCACTCAGCCACCTGCGGTACTGGATCT
ACCGGCTCCGCAAGGAGTCGCGTCCGCTCGTGACGGAGGCGGCCAGGTCGTCCGATCAAGCCCCCGTGCAGGTGACCGCGGAGAAGGGCTCGCGTCTGCT
GCCGGTGCGCGTGGTAGCGTCCGCGGCGAAGCCGCGGACCACCGTGATCGCGGCGGCCGGCGGCGACGAGCTCCTCGAGCTGGCCCTCCCGTCCGGCGTC
CGAGTCCGCTTCCCCGCGGGAACCGACCTTCGGTACCTCCGGGCCCTCGCCGCGGCGCTGTAGCCCGTGATCACCATCCCGCGGTCGGTCCGCATCTTCA
TCGGGAAGAACCCGATAGACATGCGGAAGTCCATCGACGGCCTGATGAGCGTGGTCCAGGAGGAGCTCCGCCAGGACGCCTACTCGGGCCATCTCTTCGT
GTTCCTGTCGAGGCGCGCGGACCGCGTGAAGATCCTCGCCTGGGACAAGGGCGGCTTCGTCCTTCTCTACAAGCGACTCGAGCGCGGGCAGTTCAAACTG
CCGCACATCGGGCAGGACACGATGGCCGTCGAGATCGACTCGACACAGCTCGCGATGCTGCTCGACGGGATCGAGTTCGGCCGCGTTCGTCGGCCCGCGC
ACTGGGAGCCGCCGTCCCAGGCGGATCGTCCCGCCTGCCGTCCCATGGACAAGCCGATCTGAGTGTGATCGAACATGGTTGATGGGGAGCGGCGATCACG
ACTGCGAGTGGCGCGAGCGGGCCGAGAGCCTTGCCGTTCAGCTCGACGCCGCGCAGAAGACCATCGCCTCCCAGACGCAGACGATCCAGGCCCAGAGCGA
GTCGCTCACGAAGCTGAGCGAGCAGTTCGCATCGGTAAAGGGCACCGTCGAGAAGCTCCAGCGGCACGTCTTCGGGAAGCGCTCCGAGAAGATTACGCCG
CTCGCGACGGCGCTCCGTGACCCCGCGCGCGCCGAGGCCGACCGCATCGCGGCGCTGCAGACGCGGCGGGAGAACGCGGAGAAGAAGCGCCAACTCGTCA
CCCGGAAGATCGAGCACAAGGTCCGCGAGGACCAGAAGGTCTGTCCGAAGTGCGGAGGCCGAGACTTCTCAAAGCTCGGCGACGGGGCCATGAGCGAGCT
GTACGAGCTCGTTCCGGCGATCGTGGAGCGACAGCTCCACATCCAGGAGAAGTTGCGCTGCAAGTGCGGCGAGACCGTCATCACCGCGGACGGGCCGACG
AAGGTCTTCGACAAGGCCCGCGTCGGACCCACCTTCATGGCGCAGGTCGCGGTGTCGAAGTGCGCCGACGCGTTGCCCCTTCATCGCCAGGCAAAGGCGT
ACAGGCGCGTCGGCGTACAGGTGAACGACTCGACGCTCGGCGACTACTTCCACCGCACGGCCGAGATCACCAAACCCATCTCCGACCGGCTGCTCGACGT
GGTCGCGGAGAAGGAGATCGTCCTCGCCGACGAGACGTCGCACCGCGTGCAGGCGAAGGGGAAGACGCGCCGCTCGTGGCTCTGGAGCTTCATCGCAAAG
GACGAGGACGAGCGCGAGATGATCGCGTACGTCTTCTCGCCGAGCCGCTCGAGCGAGACGCCCGAGCGCGTGCTGGAGGGCACCTCCGGCAAGCTCGTCG
CCGACGCGTACAAGGGCTACGACCGCGTGACCATGCCCGGCCGGCGTGTCCGCGCCGGCTGCCTCGCGCACGTCCGCCGGAAGTTCTTCGATGCCCAGTC
CGTCGCGCCCGATGCCGCGAAGCAGGCGATGGACTTCATCCTCGAGGTCTACAAGATCGAGCGGGCCGCGCTCGACGCCGATCTCCTCGGCACGCCCGAG
CACCTGGAGATGCGCCAGAGCGCGAGCCGCGCGGTGATGGACGACTTCAAGGCGTGGCTCGACGCGGAGCAGCCACGGCATCCGCCGAAGGGGCCGATGG
GCGAGGCGATCAACTACGCGCTCGGCCAGTGGGACGCGCTGACGCTCTTCCTCACCGATCCGCACCTGCCCATCGACAACAACGCCTCCGAGCGCGCGCT
CCGCGTGGCCGCGCTCGGACGGAAGAACTTCCTCTTCGTCGGCACGAACGAAGCCGGCGAGAACCTCGCCGGCCTGTACTCGCTCATCGCGACCTGCGAA
GCGAACGGCGTGAACCCAGTCGACTACCTCGCCGACGTGCTCATCCGCGTGCAGACGCACCCGGCCTCGCAGATCGACGAGCTGCTGCCGCACAACTGGA
CGCCCCCACCGATACGCGCGCCCTCGTGACGTCGCCTGGTGCACGGTCCGATGTAGCATCGGCTCGCCGGCGGATCCTTTCGGAGGTCGACGGGCATGGA
TGTGGAACGCTTTTTGGACCCAGGGTACCAGTTCGTTCGGGCTTGCCTCGTCGATGAGAAGGGCTTTCCGCCGTTTGCGGTGCTCTTGGCTCCCGATCAG
ACGACGTCAACGCTCATCGCGGACGATGGCAACATCGAGACCGTTCGAGCGGCTATTGTTAACCGCGACCGGAGCGGCATTCTCGCGGTCGGGCTCTTCT
CGATGGTCGACCTGCAAGCCGAACAAGGAGCCACGCAGCGCGCCATCTGCCTTCATCTCGACGTGCCGCAGGGCTTGAACAGAATCGTTCTCACGCCGTA
TCGGCTCGAAGGCGGCACACTCGTGACGGGGCAGCGTTCATCGTCGAGGCTTCGGAGCGGATCCTGCCGGCACGTTGAATCGACGCATCGAATCCGGCCG
CAACACGTCGATCACCGGCCGGTTAC

Protein section

ORF number : 3

ORF 1

Length		Begin	End	Strand	Fusion ORF
387 bp	128 aa	77	463	+	No

ORF function : Accessory Gene
AG : IS66 TnpA

ORF sequence :

MAEQDRTRWTKLVADFESSDLTQRDFATERGISLSHLRYWIYRLRKESRPLVTEAARSSDQAPVQVTAEKGSRLLPVRVVASAAKPRTTVIAAAGGDELL
ELALPSGVRVRFPAGTDLRYLRALAAAL

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
396 bp	131 aa	467	862	+	No

ORF function : Accessory Gene
AG : IS66 TnpB

ORF sequence :

MITIPRSVRIFIGKNPIDMRKSIDGLMSVVQEELRQDAYSGHLFVFLSRRADRVKILAWDKGGFVLLYKRLERGQFKLPHIGQDTMAVEIDSTQLAMLLD
GIEFGRVRRPAHWEPPSQADRPACRPMDKPI

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
1548 bp	515 aa	882	2429	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MGSGDHDCEWRERAESLAVQLDAAQKTIASQTQTIQAQSESLTKLSEQFASVKGTVEKLQRHVFGKRSEKITPLATALRDPARAEADRIAALQTRRENAE
KKRQLVTRKIEHKVREDQKVCPKCGGRDFSKLGDGAMSELYELVPAIVERQLHIQEKLRCKCGETVITADGPTKVFDKARVGPTFMAQVAVSKCADALPL
HRQAKAYRRVGVQVNDSTLGDYFHRTAEITKPISDRLLDVVAEKEIVLADETSHRVQAKGKTRRSWLWSFIAKDEDEREMIAYVFSPSRSSETPERVLEG
TSGKLVADAYKGYDRVTMPGRRVRAGCLAHVRRKFFDAQSVAPDAAKQAMDFILEVYKIERAALDADLLGTPEHLEMRQSASRAVMDDFKAWLDAEQPRH
PPKGPMGEAINYALGQWDALTLFLTDPHLPIDNNASERALRVAALGRKNFLFVGTNEAGENLAGLYSLIATCEANGVNPVDYLADVLIRVQTHPASQIDE
LLPHNWTPPPIRAPS

Blast result :

Comments

ISAnsp12 is 77% (ORFA), 90% (ORFB) and 88% (ORFC, the transposase) aa similar to ISAnsp3.

References

1] ISfinder annotation (2009)
2] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Glavina del Rio,T., Dalin,E., Tice,H., Pitluck,S., Sims,D., Brettin,T., Bruce,D., Detter,J.C., Han,C., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Lykidis,A., Fields,M. and Richardson,P.(2007) Direct submission GenBank.