ISAnsp12

  • Family IS66
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_009675 ND Anaeromyxobacter sp.
Anaeromyxobacter sp. Fw109-5
DNA section
IS Length : 2926 bp

Ends


IR Length : 20/22

IRL : GTAACCGCCCGGGGATCGACGTCTGGCAGGGCCGACCGGCCGGGCTGATG
IRR : GTAACCGGCCGGTGATCGACGTGTTGCGGCCGGATTCGATGCGTCGATTC

Insertion site


Left flankDirect repeatRight flankDR Length
CTTGCTGTGACGCGCTGGCGCTCGCTGC8

DNA sequence

GTAACCGCCCGGGGATCGACGTCTGGCAGGGCCGACCGGCCGGGCTGATGCTCCTCCGCTCACGACGGAGGACGGCATGGCCGAGCAGGATCGGACGCGG
TGGACGAAGCTTGTGGCGGACTTCGAGTCGAGCGACCTGACGCAGCGCGACTTCGCGACGGAGCGCGGGATCTCACTCAGCCACCTGCGGTACTGGATCT
ACCGGCTCCGCAAGGAGTCGCGTCCGCTCGTGACGGAGGCGGCCAGGTCGTCCGATCAAGCCCCCGTGCAGGTGACCGCGGAGAAGGGCTCGCGTCTGCT
GCCGGTGCGCGTGGTAGCGTCCGCGGCGAAGCCGCGGACCACCGTGATCGCGGCGGCCGGCGGCGACGAGCTCCTCGAGCTGGCCCTCCCGTCCGGCGTC
CGAGTCCGCTTCCCCGCGGGAACCGACCTTCGGTACCTCCGGGCCCTCGCCGCGGCGCTGTAGCCCGTGATCACCATCCCGCGGTCGGTCCGCATCTTCA
TCGGGAAGAACCCGATAGACATGCGGAAGTCCATCGACGGCCTGATGAGCGTGGTCCAGGAGGAGCTCCGCCAGGACGCCTACTCGGGCCATCTCTTCGT
GTTCCTGTCGAGGCGCGCGGACCGCGTGAAGATCCTCGCCTGGGACAAGGGCGGCTTCGTCCTTCTCTACAAGCGACTCGAGCGCGGGCAGTTCAAACTG
CCGCACATCGGGCAGGACACGATGGCCGTCGAGATCGACTCGACACAGCTCGCGATGCTGCTCGACGGGATCGAGTTCGGCCGCGTTCGTCGGCCCGCGC
ACTGGGAGCCGCCGTCCCAGGCGGATCGTCCCGCCTGCCGTCCCATGGACAAGCCGATCTGAGTGTGATCGAACATGGTTGATGGGGAGCGGCGATCACG
ACTGCGAGTGGCGCGAGCGGGCCGAGAGCCTTGCCGTTCAGCTCGACGCCGCGCAGAAGACCATCGCCTCCCAGACGCAGACGATCCAGGCCCAGAGCGA
GTCGCTCACGAAGCTGAGCGAGCAGTTCGCATCGGTAAAGGGCACCGTCGAGAAGCTCCAGCGGCACGTCTTCGGGAAGCGCTCCGAGAAGATTACGCCG
CTCGCGACGGCGCTCCGTGACCCCGCGCGCGCCGAGGCCGACCGCATCGCGGCGCTGCAGACGCGGCGGGAGAACGCGGAGAAGAAGCGCCAACTCGTCA
CCCGGAAGATCGAGCACAAGGTCCGCGAGGACCAGAAGGTCTGTCCGAAGTGCGGAGGCCGAGACTTCTCAAAGCTCGGCGACGGGGCCATGAGCGAGCT
GTACGAGCTCGTTCCGGCGATCGTGGAGCGACAGCTCCACATCCAGGAGAAGTTGCGCTGCAAGTGCGGCGAGACCGTCATCACCGCGGACGGGCCGACG
AAGGTCTTCGACAAGGCCCGCGTCGGACCCACCTTCATGGCGCAGGTCGCGGTGTCGAAGTGCGCCGACGCGTTGCCCCTTCATCGCCAGGCAAAGGCGT
ACAGGCGCGTCGGCGTACAGGTGAACGACTCGACGCTCGGCGACTACTTCCACCGCACGGCCGAGATCACCAAACCCATCTCCGACCGGCTGCTCGACGT
GGTCGCGGAGAAGGAGATCGTCCTCGCCGACGAGACGTCGCACCGCGTGCAGGCGAAGGGGAAGACGCGCCGCTCGTGGCTCTGGAGCTTCATCGCAAAG
GACGAGGACGAGCGCGAGATGATCGCGTACGTCTTCTCGCCGAGCCGCTCGAGCGAGACGCCCGAGCGCGTGCTGGAGGGCACCTCCGGCAAGCTCGTCG
CCGACGCGTACAAGGGCTACGACCGCGTGACCATGCCCGGCCGGCGTGTCCGCGCCGGCTGCCTCGCGCACGTCCGCCGGAAGTTCTTCGATGCCCAGTC
CGTCGCGCCCGATGCCGCGAAGCAGGCGATGGACTTCATCCTCGAGGTCTACAAGATCGAGCGGGCCGCGCTCGACGCCGATCTCCTCGGCACGCCCGAG
CACCTGGAGATGCGCCAGAGCGCGAGCCGCGCGGTGATGGACGACTTCAAGGCGTGGCTCGACGCGGAGCAGCCACGGCATCCGCCGAAGGGGCCGATGG
GCGAGGCGATCAACTACGCGCTCGGCCAGTGGGACGCGCTGACGCTCTTCCTCACCGATCCGCACCTGCCCATCGACAACAACGCCTCCGAGCGCGCGCT
CCGCGTGGCCGCGCTCGGACGGAAGAACTTCCTCTTCGTCGGCACGAACGAAGCCGGCGAGAACCTCGCCGGCCTGTACTCGCTCATCGCGACCTGCGAA
GCGAACGGCGTGAACCCAGTCGACTACCTCGCCGACGTGCTCATCCGCGTGCAGACGCACCCGGCCTCGCAGATCGACGAGCTGCTGCCGCACAACTGGA
CGCCCCCACCGATACGCGCGCCCTCGTGACGTCGCCTGGTGCACGGTCCGATGTAGCATCGGCTCGCCGGCGGATCCTTTCGGAGGTCGACGGGCATGGA
TGTGGAACGCTTTTTGGACCCAGGGTACCAGTTCGTTCGGGCTTGCCTCGTCGATGAGAAGGGCTTTCCGCCGTTTGCGGTGCTCTTGGCTCCCGATCAG
ACGACGTCAACGCTCATCGCGGACGATGGCAACATCGAGACCGTTCGAGCGGCTATTGTTAACCGCGACCGGAGCGGCATTCTCGCGGTCGGGCTCTTCT
CGATGGTCGACCTGCAAGCCGAACAAGGAGCCACGCAGCGCGCCATCTGCCTTCATCTCGACGTGCCGCAGGGCTTGAACAGAATCGTTCTCACGCCGTA
TCGGCTCGAAGGCGGCACACTCGTGACGGGGCAGCGTTCATCGTCGAGGCTTCGGAGCGGATCCTGCCGGCACGTTGAATCGACGCATCGAATCCGGCCG
CAACACGTCGATCACCGGCCGGTTAC
Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
387 bp128 aa77463+No
ORF function : Accessory Gene
AG : IS66 TnpA

ORF sequence :

MAEQDRTRWTKLVADFESSDLTQRDFATERGISLSHLRYWIYRLRKESRPLVTEAARSSDQAPVQVTAEKGSRLLPVRVVASAAKPRTTVIAAAGGDELL
ELALPSGVRVRFPAGTDLRYLRALAAAL

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
396 bp131 aa467862+No
ORF function : Accessory Gene
AG : IS66 TnpB

ORF sequence :

MITIPRSVRIFIGKNPIDMRKSIDGLMSVVQEELRQDAYSGHLFVFLSRRADRVKILAWDKGGFVLLYKRLERGQFKLPHIGQDTMAVEIDSTQLAMLLD
GIEFGRVRRPAHWEPPSQADRPACRPMDKPI

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1548 bp515 aa8822429+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MGSGDHDCEWRERAESLAVQLDAAQKTIASQTQTIQAQSESLTKLSEQFASVKGTVEKLQRHVFGKRSEKITPLATALRDPARAEADRIAALQTRRENAE
KKRQLVTRKIEHKVREDQKVCPKCGGRDFSKLGDGAMSELYELVPAIVERQLHIQEKLRCKCGETVITADGPTKVFDKARVGPTFMAQVAVSKCADALPL
HRQAKAYRRVGVQVNDSTLGDYFHRTAEITKPISDRLLDVVAEKEIVLADETSHRVQAKGKTRRSWLWSFIAKDEDEREMIAYVFSPSRSSETPERVLEG
TSGKLVADAYKGYDRVTMPGRRVRAGCLAHVRRKFFDAQSVAPDAAKQAMDFILEVYKIERAALDADLLGTPEHLEMRQSASRAVMDDFKAWLDAEQPRH
PPKGPMGEAINYALGQWDALTLFLTDPHLPIDNNASERALRVAALGRKNFLFVGTNEAGENLAGLYSLIATCEANGVNPVDYLADVLIRVQTHPASQIDE
LLPHNWTPPPIRAPS

 

Blast result :
Comments
ISAnsp12 is 77% (ORFA), 90% (ORFB) and 88% (ORFC, the transposase) aa similar to ISAnsp3.
References
1] ISfinder annotation (2009)
2] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Glavina del Rio,T., Dalin,E., Tice,H., Pitluck,S., Sims,D., Brettin,T., Bruce,D., Detter,J.C., Han,C., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Lykidis,A., Fields,M. and Richardson,P.(2007) Direct submission GenBank.