ISAnsp11
- Family IS3
- Group IS3
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_009675 | ND | Anaeromyxobacter sp. | Anaeromyxobacter sp. Fw109-5 |
DNA section
IS Length : 1510 bp
Ends
IR Length : 18/20
IRL : TGCCAACGTGAGTGAGACAGCGACCCCCACGGAGTTTTGTGTAGAAGGGC
IRR : TGTCAACGTGAATGAGACAGGGTGCTGCTGAGATTACTGACGCGCCTCCT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CGGCCCTCTC | GGTTCCGCGG | 0 |
DNA sequence
TGCCAACGTGAGTGAGACAGCGACCCCCACGGAGTTTTGTGTAGAAGGGCGAGCTTGGACTTCACGGTGAGCAGCAGCGCAGTGATCGGGTTGGTTGGGC
AGGAGACCGAGGTGACCGCGAAGGCGCGCAGGCGGCAGTTTACCGCCGAGTACAAGCGCAAGGTCCTCGCCGAGGCCGAGGCATGCACGAAGACGGGCGA
GATCGGCGCCCTGCTCCGTCGCGAGGGCCTGTACTCGTCGCACCTCGTGGAGTGGCGGCGGGCCCGTGACAGCGGTGCGCTGGCCGGGCTCGCGCCCAGG
AAGCGCGGACCGGCCGCGAAGGTCCCGCACCCGCTGGAGCGGAAGGTCGCCGAGCTCGAGCGCGAGGTCGCGCGGGCCACGCGGCGCGCAGAGCGCGCGG
AGGGTCTCGTCGAACTCCAAAAAAAAGTCTCGGAGCTGCTGGGGATCCAACTGCCGAAGCCCGACGAGGAGAAGTGATGCAGGCGGTCACCGATCTCGGC
GCCCGGCTTGGCATCGCGGCGTCGTGCGCCGCGCTGGGGCTGCCGAAGGCCACCTACTACCGCGCGCTCAAGCCGCGCCCGCCGCGGCGGACGCGGACCT
ACCACCGCGCGCTTGGCGCCGACGAGCACCGTGCCGTGCTCGACGTGCTTCACGAGCCTCGCTTTGCGGAGCTCGCGCCTGCCCAGGTCTACGCCACGCT
GCTCGACGAGGGCCGGTACCTCTGCTCGGAGCGCACGATGTACCGCGTCCTCGCTGAGAACGCGGAGGTCCGCGAGCGGCGGAACCAGCTGCGGCACCCG
AAGTACAAGGCGCCCGAGCTACTCGCCACCGCGCCGAACCAGCTCTGGAGCTGGGACATCACGAGCTGCTCGGCCCGCAGAAGTGGACCTACTTCTATCT
GTATGTGATCCTCGACGTTTACAGCCGGTACGTGGTCGGCTGGATGCTCGCGCACCGGGAGACGGCGGTGCTCGCCGAGCGGCTCATCCGCGAAACCTGC
GAACGCCAAGGCATCCAGCGCGACCAGCTCACCGTCCACGCCGACCGCGGAACCTCGATGACCTCGAAGCCGGTGGCGCTGATGCTCGCCGACCTGGGCG
TCACGAAGAGCCACTCGCGGCCGCACGTCTCGAACGACAACCCGTTCTCCGAGTCGCAGTTCAAGACGATGAAGTACCGGCCCGACTTCCCGGAACGCTT
CGGCGCCATCGAGGACGGGCGCGCGCACTGCGCCGACTTCTTCGGTTGGTACAACACCGAGCACCGCCACGGCGGGCTCGGGCTGGTGACGCCGCACGAC
GTCCACCACGGCCTCGCGGCCGCCCGGCTCCAGGCGCGCGACGTGGTCCTCGCGGCCGCCTTCGCCGCCCGGCCTGAGCGGTTTCCCGCCGGCGCGCCGT
CGGCGGGCGCGGTGCCGTGCGAGGTCTGGATCAACAAGCCGCAGCCGGCTTCACCCACAGAGGAGGCGCGTCAGTAATCTCAGCAGCACCCTGTCTCATT
CACGTTGACA
AGGAGACCGAGGTGACCGCGAAGGCGCGCAGGCGGCAGTTTACCGCCGAGTACAAGCGCAAGGTCCTCGCCGAGGCCGAGGCATGCACGAAGACGGGCGA
GATCGGCGCCCTGCTCCGTCGCGAGGGCCTGTACTCGTCGCACCTCGTGGAGTGGCGGCGGGCCCGTGACAGCGGTGCGCTGGCCGGGCTCGCGCCCAGG
AAGCGCGGACCGGCCGCGAAGGTCCCGCACCCGCTGGAGCGGAAGGTCGCCGAGCTCGAGCGCGAGGTCGCGCGGGCCACGCGGCGCGCAGAGCGCGCGG
AGGGTCTCGTCGAACTCCAAAAAAAAGTCTCGGAGCTGCTGGGGATCCAACTGCCGAAGCCCGACGAGGAGAAGTGATGCAGGCGGTCACCGATCTCGGC
GCCCGGCTTGGCATCGCGGCGTCGTGCGCCGCGCTGGGGCTGCCGAAGGCCACCTACTACCGCGCGCTCAAGCCGCGCCCGCCGCGGCGGACGCGGACCT
ACCACCGCGCGCTTGGCGCCGACGAGCACCGTGCCGTGCTCGACGTGCTTCACGAGCCTCGCTTTGCGGAGCTCGCGCCTGCCCAGGTCTACGCCACGCT
GCTCGACGAGGGCCGGTACCTCTGCTCGGAGCGCACGATGTACCGCGTCCTCGCTGAGAACGCGGAGGTCCGCGAGCGGCGGAACCAGCTGCGGCACCCG
AAGTACAAGGCGCCCGAGCTACTCGCCACCGCGCCGAACCAGCTCTGGAGCTGGGACATCACGAGCTGCTCGGCCCGCAGAAGTGGACCTACTTCTATCT
GTATGTGATCCTCGACGTTTACAGCCGGTACGTGGTCGGCTGGATGCTCGCGCACCGGGAGACGGCGGTGCTCGCCGAGCGGCTCATCCGCGAAACCTGC
GAACGCCAAGGCATCCAGCGCGACCAGCTCACCGTCCACGCCGACCGCGGAACCTCGATGACCTCGAAGCCGGTGGCGCTGATGCTCGCCGACCTGGGCG
TCACGAAGAGCCACTCGCGGCCGCACGTCTCGAACGACAACCCGTTCTCCGAGTCGCAGTTCAAGACGATGAAGTACCGGCCCGACTTCCCGGAACGCTT
CGGCGCCATCGAGGACGGGCGCGCGCACTGCGCCGACTTCTTCGGTTGGTACAACACCGAGCACCGCCACGGCGGGCTCGGGCTGGTGACGCCGCACGAC
GTCCACCACGGCCTCGCGGCCGCCCGGCTCCAGGCGCGCGACGTGGTCCTCGCGGCCGCCTTCGCCGCCCGGCCTGAGCGGTTTCCCGCCGGCGCGCCGT
CGGCGGGCGCGGTGCCGTGCGAGGTCTGGATCAACAAGCCGCAGCCGGCTTCACCCACAGAGGAGGCGCGTCAGTAATCTCAGCAGCACCCTGTCTCATT
CACGTTGACA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 423 bp | 140 aa | 55 | 477 | + | No |
Description : First part of the transposase
ORF sequence :
MDFTVSSSAVIGLVGQETEVTAKARRRQFTAEYKRKVLAEAEACTKTGEIGALLRREGLYSSHLVEWRRARDSGALAGLAPRKRGPAAKVPHPLERKVAE
LEREVARATRRAERAEGLVELQKKVSELLGIQLPKPDEEK
LEREVARATRRAERAEGLVELQKKVSELLGIQLPKPDEEK
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1208 bp | 402 aa | 270 | 1477 | + | No |
Description : Second part of the transposase
ORF sequence :
QRCAGRARAQEARTGREGPAPAGAEGRRARARGRAGHAARRARGGSRRTPKKSLGAAGDPTAEARRGEVMQAVTDLGARLGIAASCAALGLPKATYYRAL
KPRPPRRTRTYHRALGADEHRAVLDVLHEPRFAELAPAQVYATLLDEGRYLCSERTMYRVLAENAEVRERRNQLRHPKYKAPELLATAPNQLWSWDITEL
LGPQKWTYFYLYVILDVYSRYVVGWMLAHRETAVLAERLIRETCERQGIQRDQLTVHADRGTSMTSKPVALMLADLGVTKSHSRPHVSNDNPFSESQFKT
MKYRPDFPERFGAIEDGRAHCADFFGWYNTEHRHGGLGLVTPHDVHHGLAAARLQARDVVLAAAFAARPERFPAGAPSAGAVPCEVWINKPQPASPTEEA
RQ
KPRPPRRTRTYHRALGADEHRAVLDVLHEPRFAELAPAQVYATLLDEGRYLCSERTMYRVLAENAEVRERRNQLRHPKYKAPELLATAPNQLWSWDITEL
LGPQKWTYFYLYVILDVYSRYVVGWMLAHRETAVLAERLIRETCERQGIQRDQLTVHADRGTSMTSKPVALMLADLGVTKSHSRPHVSNDNPFSESQFKT
MKYRPDFPERFGAIEDGRAHCADFFGWYNTEHRHGGLGLVTPHDVHHGLAAARLQARDVVLAAAFAARPERFPAGAPSAGAVPCEVWINKPQPASPTEEA
RQ
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1423 bp | 474 aa | 55 | 1477 | + | Yes |
Chemistry : DDE
ORF sequence :
MDFTVSSSAVIGLVGQETEVTAKARRRQFTAEYKRKVLAEAEACTKTGEIGALLRREGLYSSHLVEWRRARDSGALAGLAPRKRGPAAKVPHPLERKVAE
LEREVARATRRAERAEGLVELQKKSLGAAGDPTAEARRGEVMQAVTDLGARLGIAASCAALGLPKATYYRALKPRPPRRTRTYHRALGADEHRAVLDVLH
EPRFAELAPAQVYATLLDEGRYLCSERTMYRVLAENAEVRERRNQLRHPKYKAPELLATAPNQLWSWDITELLGPQKWTYFYLYVILDVYSRYVVGWMLA
HRETAVLAERLIRETCERQGIQRDQLTVHADRGTSMTSKPVALMLADLGVTKSHSRPHVSNDNPFSESQFKTMKYRPDFPERFGAIEDGRAHCADFFGWY
NTEHRHGGLGLVTPHDVHHGLAAARLQARDVVLAAAFAARPERFPAGAPSAGAVPCEVWINKPQPASPTEEARQ
LEREVARATRRAERAEGLVELQKKSLGAAGDPTAEARRGEVMQAVTDLGARLGIAASCAALGLPKATYYRALKPRPPRRTRTYHRALGADEHRAVLDVLH
EPRFAELAPAQVYATLLDEGRYLCSERTMYRVLAENAEVRERRNQLRHPKYKAPELLATAPNQLWSWDITELLGPQKWTYFYLYVILDVYSRYVVGWMLA
HRETAVLAERLIRETCERQGIQRDQLTVHADRGTSMTSKPVALMLADLGVTKSHSRPHVSNDNPFSESQFKTMKYRPDFPERFGAIEDGRAHCADFFGWY
NTEHRHGGLGLVTPHDVHHGLAAARLQARDVVLAAAFAARPERFPAGAPSAGAVPCEVWINKPQPASPTEEARQ
Blast result :
Comments
ISAnsp11 is 72% aa similar to ISGur10. The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
The ORF was obtained by joining (270-863;863-1477).
The ORF was obtained by joining (270-863;863-1477).
References
1] ISfinder annotation (2009)
2] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Glavina del Rio,T., Dalin,E., Tice,H., Pitluck,S., Sims,D., Brettin,T., Bruce,D., Detter,J.C., Han,C., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Lykidis,A., Fields,M. and Richardson,P. (2007) Direct submission GenBank.
2] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Glavina del Rio,T., Dalin,E., Tice,H., Pitluck,S., Sims,D., Brettin,T., Bruce,D., Detter,J.C., Han,C., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Lykidis,A., Fields,M. and Richardson,P. (2007) Direct submission GenBank.