ISMdi14
- Family IS110
- Group IS1111
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Methylobacterium dichloromethanicum | Methylobacterium dichloromethanicum DM4 |
DNA section
IS Length : 1316 bp
Ends
IR Length : 13/15
IRL : ttgggtaATGGACGGCAGCTCTTCCGCGGAAGGCCGATAGGATCGGCTCC
IRR : ---gtcAATGGGCGGCAACTCGGTCCTGAGCGCTCGACGCTTTCGGGTGC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GAGTTTTTCAACA | TCATTAGAGAACAC | 0 |
DNA sequence
TTGGGTAATGGACGGCAGCTCTTCCGCGGAAGGCCGATAGGATCGGCTCCGCTGCCGGAAGAGGAGGCTGATCATGGAGTATTTCGCTGGCTTGGATGTC
TCCTTGGAGGAAACGCACGTCTGCGTGGTCGACCGCGACGGAGTAGTCATCCATCAGGCGAAGGTGGCCTCCACCCCTGCCGACATCGCGCAGGAACTCG
CGAAGGCACCCGCCTGCCGCCGGGTGGTGTTCGAGACCGGCCGGATGGCTCCGATACTGTTCCACGGCTTGGTCGAGCACGATGTGCCGGTCGTCTGCGT
CGAGAGCCGGCAGGCTTACCAAGGCCTGAAGTCGCTCACGACGCACAAGACCGACCGCAACGATGCGCGCGGTCTCGCTCACCTGGCACGAACGGGCTTC
TTCAAACCGGTGCACGTGAAGTCGCTGCCGGCACACGCCGTGAGGTCCTTGATCATCGCTCGCAAGAAGCTGGTCGGCCAGCGGGTCACGCTGGAGAACC
AGATCCGCGGCCTTGCCGTCGTGTTCGGGGTGCGGCTGCCGCGGGCGCTCAGCCCTGCCTTCATCGAGCAGGCACTGCTTTCGAGCGAGGGGATTAACGG
GCTGTCCGCCGCCATGCGCGGCCTGATCGGCGCGCGAGCCGCAGTGCTGGCGGTTGTCGCAGCCATCGACGCAGATATCAAGAAGATCGTCCGGATGTCG
GAGGCCTGCCGTCGCCTGATGACGATCCCGGGTGTCGGTCAGCTCACTGCGCTCGCCTTCACGGCAGCCGTCGATGATCCCGAGCGCTTCAGGCGCTCTC
GCGACGTCGGGCCGTATCTCGGCTTGGTTCCACGATGCTACCAGTCGGGCGAGGTCAACTACACCGGCAGCATCTCGAAGTGCGGCGACCGCCGGGTCAG
AACGCTGCTCTACGAAGCCGCCAACGTGATGCTGACGCGCTGCAGGCGGCCTCTGAAGCTGAAGGAGTGGGCTCTGGCCATCGCGAGGCGCTCGACGATG
CGCAAGGCTCGCATCGCGCTCGCGCGCCGGCTGGCAATCATCATGCACGCGATGCTGCGCCAGGGCACTGAGTTCAAGGCCGCGTAACGGTCCACGTCGC
CGGACAGGAGGCCGAACCGAGCTCCCGAGCGGGAGCGACGCCCGAGGGAGGGAGCGGAAGACGGCGCCGATTTTGTAGCAGGCGGCCTTGCGCCGACTGC
GGTTTCAACCGAGCCGCTCTGCACCCAGCTGACCCCATCAAGTGCCGCACGAGCACGTAGAGAACGCAGGCACCCGAAAGCGTCGAGCGCTCAGGACCGA
GTTGCCGCCCATTGAC
TCCTTGGAGGAAACGCACGTCTGCGTGGTCGACCGCGACGGAGTAGTCATCCATCAGGCGAAGGTGGCCTCCACCCCTGCCGACATCGCGCAGGAACTCG
CGAAGGCACCCGCCTGCCGCCGGGTGGTGTTCGAGACCGGCCGGATGGCTCCGATACTGTTCCACGGCTTGGTCGAGCACGATGTGCCGGTCGTCTGCGT
CGAGAGCCGGCAGGCTTACCAAGGCCTGAAGTCGCTCACGACGCACAAGACCGACCGCAACGATGCGCGCGGTCTCGCTCACCTGGCACGAACGGGCTTC
TTCAAACCGGTGCACGTGAAGTCGCTGCCGGCACACGCCGTGAGGTCCTTGATCATCGCTCGCAAGAAGCTGGTCGGCCAGCGGGTCACGCTGGAGAACC
AGATCCGCGGCCTTGCCGTCGTGTTCGGGGTGCGGCTGCCGCGGGCGCTCAGCCCTGCCTTCATCGAGCAGGCACTGCTTTCGAGCGAGGGGATTAACGG
GCTGTCCGCCGCCATGCGCGGCCTGATCGGCGCGCGAGCCGCAGTGCTGGCGGTTGTCGCAGCCATCGACGCAGATATCAAGAAGATCGTCCGGATGTCG
GAGGCCTGCCGTCGCCTGATGACGATCCCGGGTGTCGGTCAGCTCACTGCGCTCGCCTTCACGGCAGCCGTCGATGATCCCGAGCGCTTCAGGCGCTCTC
GCGACGTCGGGCCGTATCTCGGCTTGGTTCCACGATGCTACCAGTCGGGCGAGGTCAACTACACCGGCAGCATCTCGAAGTGCGGCGACCGCCGGGTCAG
AACGCTGCTCTACGAAGCCGCCAACGTGATGCTGACGCGCTGCAGGCGGCCTCTGAAGCTGAAGGAGTGGGCTCTGGCCATCGCGAGGCGCTCGACGATG
CGCAAGGCTCGCATCGCGCTCGCGCGCCGGCTGGCAATCATCATGCACGCGATGCTGCGCCAGGGCACTGAGTTCAAGGCCGCGTAACGGTCCACGTCGC
CGGACAGGAGGCCGAACCGAGCTCCCGAGCGGGAGCGACGCCCGAGGGAGGGAGCGGAAGACGGCGCCGATTTTGTAGCAGGCGGCCTTGCGCCGACTGC
GGTTTCAACCGAGCCGCTCTGCACCCAGCTGACCCCATCAAGTGCCGCACGAGCACGTAGAGAACGCAGGCACCCGAAAGCGTCGAGCGCTCAGGACCGA
GTTGCCGCCCATTGAC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1014 bp | 337 aa | 74 | 1087 | + | No |
Chemistry : DEDD
ORF sequence :
MEYFAGLDVSLEETHVCVVDRDGVVIHQAKVASTPADIAQELAKAPACRRVVFETGRMAPILFHGLVEHDVPVVCVESRQAYQGLKSLTTHKTDRNDARG
LAHLARTGFFKPVHVKSLPAHAVRSLIIARKKLVGQRVTLENQIRGLAVVFGVRLPRALSPAFIEQALLSSEGINGLSAAMRGLIGARAAVLAVVAAIDA
DIKKIVRMSEACRRLMTIPGVGQLTALAFTAAVDDPERFRRSRDVGPYLGLVPRCYQSGEVNYTGSISKCGDRRVRTLLYEAANVMLTRCRRPLKLKEWA
LAIARRSTMRKARIALARRLAIIMHAMLRQGTEFKAA
LAHLARTGFFKPVHVKSLPAHAVRSLIIARKKLVGQRVTLENQIRGLAVVFGVRLPRALSPAFIEQALLSSEGINGLSAAMRGLIGARAAVLAVVAAIDA
DIKKIVRMSEACRRLMTIPGVGQLTALAFTAAVDDPERFRRSRDVGPYLGLVPRCYQSGEVNYTGSISKCGDRRVRTLLYEAANVMLTRCRRPLKLKEWA
LAIARRSTMRKARIALARRLAIIMHAMLRQGTEFKAA
Blast result :
Comments
ISMdi14 is 61% aa similar to ISMex9. IR are not at the termini, in the IS sequence as given 7 nt separate IRl from the left-hand end of the element and 3 nt separate IRr from the right-hand end.
References
1] Stephane Vuilleumier et.al. Methylobacterium genome sequences: a reference blueprint to investigate microbial metabolism of C1 compounds from natural and industrial sources. (2009) PLoS ONE Submitted.