ISMdi9
- Family IS110
- Group IS1111
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Methylobacterium dichloromethanicum | Methylobacterium dichloromethanicum DM4 |
DNA section
IS Length : 1399 bp
Ends
IR Length : 8
IRL : agatgccGTAATGGGCCTGCCGCCGGCGAGTAGCGAGGCGGGGCGGCTGT
IRR : ----tctGTAATGGGTCGTGTTGCGTCAAGTCCCGAGTGATCGTCACGTC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CAGGTGACAT | AGCCTTATCC | 0 |
DNA sequence
AGATGCCGTAATGGGCCTGCCGCCGGCGAGTAGCGAGGCGGGGCGGCTGTCCCTCAGAGTGATGGTGTGTGGCACGGGGTTCGAGAGGCTCGGCCCCAAG
CATCATCGAGGGATAGCCATGGACTACTATGTCGGAATCGATGTCACGCTAGAACTCAGCAGCCTCTGCGTGCTCGATGCCGCAGGCCAGATTGTCTGCG
AAGCGAAGGTCGCCAGCGAGCCCGAGGCGCTGATCGGGTACCTGACCAGGCTCGCTCTGCCGCTGACCCGTATCGGCTTGGAGGCCGGCCCGCTGTCGCA
ATGGCTGCACGCCGCCCTGGTTGAGGCGGGGTTTGCGGCGGTGCTGCTGGAGACGCGTCACGTCAAGGCGGCGCTCTCGGCCATGATCGTCAAGACCGAT
CGACGGGATGCGCGCGGCATCGCCCAGCTCCTGCGGATGGGTTGGTTCCGGTCCGTGCACTGCAAATCGCCGCCGGCCCAGGAGGTGCGGGCGCTACTGG
TGGCTCGAAAGCAGCTGCAGGCCAAGATGCGAGACGTGGAACTCAGCCTACGCGGCCTCCTGCGTGGTTTTGGGCTCAAGGTCGGCGAGATCAGCAAAGG
CCAGTTCGCCGCCCGGGTTCGTATGTTGGTCACTGGTCACAGCATGCTGGAGCGGGTAGCTGAGGCGATGCTGCAGGCACGCGAGGCTCTCCGAGCGGAG
TTTTCCAAGCTGCACCGGGCCATGCTGGCGATCGTGCGTACGGATGAGGTCTGCCGGCGTCTGATGACGGTGCCGGGCGTGGGGGCGCTGATCGCCGTCA
CCTTCACCTCGGGTGTGGATGATCCGGCCCGCTTCCGGCACTCAAAGGATGTCGGCGCGCATTTCGGCCTGACGCTGCGCAAGTACCAGTCAGGCGAGAC
GGACATCACGGGTGCGGTCAGCCGCGTGGGCGACGTGATGGTGCGAACGGCCCTCTACGAAGGCGCCCACATCCTGCTGACACGCGCGGGGCGCTTCTCA
GCCCTGAAGCGCTGGGCATTGGAGGTGGCCAAGCGGCGAGGCATGAAGCGAGCGAAAGTCGCGCTGGCTCGGAAGCTGGCGGTCGTGCTGCACAGAATCT
GGGTGGATGGCACCAGCTTCCGCTGGGGCAGTGAGAGCCTGCCAGCCTGAAGGAGGCAAACCGTTCGGAGCGGGGCAGACCCGTCCTGCTCATCTGAGGT
CCCGTCGCCGGGACGCGGGGAGCGACAAAGCCGTGGCTAAGGCCGTAAACGTTCTTCGGAACGGAATACGCACCATAGATGAGCTTGCCGCCCCTGTCTG
ACCGCATGATGTGGCGGCACTTCGCTGACCACGGACAGAAGCACGACGCCGGCGACGTGACGATCACTCGGGACTTGACGCAACACGACCCATTACAGA
CATCATCGAGGGATAGCCATGGACTACTATGTCGGAATCGATGTCACGCTAGAACTCAGCAGCCTCTGCGTGCTCGATGCCGCAGGCCAGATTGTCTGCG
AAGCGAAGGTCGCCAGCGAGCCCGAGGCGCTGATCGGGTACCTGACCAGGCTCGCTCTGCCGCTGACCCGTATCGGCTTGGAGGCCGGCCCGCTGTCGCA
ATGGCTGCACGCCGCCCTGGTTGAGGCGGGGTTTGCGGCGGTGCTGCTGGAGACGCGTCACGTCAAGGCGGCGCTCTCGGCCATGATCGTCAAGACCGAT
CGACGGGATGCGCGCGGCATCGCCCAGCTCCTGCGGATGGGTTGGTTCCGGTCCGTGCACTGCAAATCGCCGCCGGCCCAGGAGGTGCGGGCGCTACTGG
TGGCTCGAAAGCAGCTGCAGGCCAAGATGCGAGACGTGGAACTCAGCCTACGCGGCCTCCTGCGTGGTTTTGGGCTCAAGGTCGGCGAGATCAGCAAAGG
CCAGTTCGCCGCCCGGGTTCGTATGTTGGTCACTGGTCACAGCATGCTGGAGCGGGTAGCTGAGGCGATGCTGCAGGCACGCGAGGCTCTCCGAGCGGAG
TTTTCCAAGCTGCACCGGGCCATGCTGGCGATCGTGCGTACGGATGAGGTCTGCCGGCGTCTGATGACGGTGCCGGGCGTGGGGGCGCTGATCGCCGTCA
CCTTCACCTCGGGTGTGGATGATCCGGCCCGCTTCCGGCACTCAAAGGATGTCGGCGCGCATTTCGGCCTGACGCTGCGCAAGTACCAGTCAGGCGAGAC
GGACATCACGGGTGCGGTCAGCCGCGTGGGCGACGTGATGGTGCGAACGGCCCTCTACGAAGGCGCCCACATCCTGCTGACACGCGCGGGGCGCTTCTCA
GCCCTGAAGCGCTGGGCATTGGAGGTGGCCAAGCGGCGAGGCATGAAGCGAGCGAAAGTCGCGCTGGCTCGGAAGCTGGCGGTCGTGCTGCACAGAATCT
GGGTGGATGGCACCAGCTTCCGCTGGGGCAGTGAGAGCCTGCCAGCCTGAAGGAGGCAAACCGTTCGGAGCGGGGCAGACCCGTCCTGCTCATCTGAGGT
CCCGTCGCCGGGACGCGGGGAGCGACAAAGCCGTGGCTAAGGCCGTAAACGTTCTTCGGAACGGAATACGCACCATAGATGAGCTTGCCGCCCCTGTCTG
ACCGCATGATGTGGCGGCACTTCGCTGACCACGGACAGAAGCACGACGCCGGCGACGTGACGATCACTCGGGACTTGACGCAACACGACCCATTACAGA
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1140 bp | 379 aa | 11 | 1150 | + | No |
Chemistry : DEDD
ORF sequence :
MGLPPASSEAGRLSLRVMVCGTGFERLGPKHHRGIAMDYYVGIDVTLELSSLCVLDAAGQIVCEAKVASEPEALIGYLTRLALPLTRIGLEAGPLSQWLH
AALVEAGFAAVLLETRHVKAALSAMIVKTDRRDARGIAQLLRMGWFRSVHCKSPPAQEVRALLVARKQLQAKMRDVELSLRGLLRGFGLKVGEISKGQFA
ARVRMLVTGHSMLERVAEAMLQAREALRAEFSKLHRAMLAIVRTDEVCRRLMTVPGVGALIAVTFTSGVDDPARFRHSKDVGAHFGLTLRKYQSGETDIT
GAVSRVGDVMVRTALYEGAHILLTRAGRFSALKRWALEVAKRRGMKRAKVALARKLAVVLHRIWVDGTSFRWGSESLPA
AALVEAGFAAVLLETRHVKAALSAMIVKTDRRDARGIAQLLRMGWFRSVHCKSPPAQEVRALLVARKQLQAKMRDVELSLRGLLRGFGLKVGEISKGQFA
ARVRMLVTGHSMLERVAEAMLQAREALRAEFSKLHRAMLAIVRTDEVCRRLMTVPGVGALIAVTFTSGVDDPARFRHSKDVGAHFGLTLRKYQSGETDIT
GAVSRVGDVMVRTALYEGAHILLTRAGRFSALKRWALEVAKRRGMKRAKVALARKLAVVLHRIWVDGTSFRWGSESLPA
Blast result :
Comments
ISMdi9 is 70% aa similar to ISMex9. IR are not at the termini, in the IS sequence as given 7 nt separate IRl from the left-hand end of the element and 3 nt separate IRr from the right-hand end.
References
1] Stephane Vuilleumier et.al. Methylobacterium genome sequences: a reference blueprint to investigate microbial metabolism of C1 compounds from natural and industrial sources. (2009) PLoS ONE Submitted.