ISAcma42

  • Family IS701
  • Group ISAba11
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_009925 ND Acaryochloris marina
Acaryochloris marina MBIC11017
DNA section
IS Length : 1140 bp

Ends


IR Length : 18/19

IRL : CAGGACTAACGCATTGACATGGACTTTATTGTGTGAGTTGTAGAGATAAA
IRR : CAGGACAAACGCATTGACAGAAATTACACCCAATGTTCAAGAGAGCTATC

Insertion site


Left flankDirect repeatRight flankDR Length
GGTTTCGGATTGTCTGTTCTATCCC5
CACTGGTGATGGTCTGTTCTGATGT5

DNA sequence

CAGGACTAACGCATTGACATGGACTTTATTGTGTGAGTTGTAGAGATAAAGGCTGTTAACGTTCTACCTCTCTTGCGAATACCCAGTAAGCCTTCAACAG
CTCATTGCACCCAAGACTTGTATGTCCGCTATCTCTTGGCTCAACCCAAAGGAGATGGGTGCAGCCATATGGCTGAAATCCTCCAAGATGTCTCTCACGA
CAGCATCAATCGCTTTTTGCTTCGAGAACGGTATGAACCCAAAGACCTATTTGATTTGCTGGTTGGTAATGGATGGGTTGAGCTTACTGGAGGCATTGTG
AGTGCTGATGATACCATCCTCGAAAAACTCTACAGCAACCCCAAGAAAATGGACTTGCTGGGATATTACTGGAGTAGCAAATATAGCAAACCCATTTTAG
GAATTCCCTTAATCACCCTGTACTACAGCAGTCCGAATGGCTTGAGAGTCCCCATCAACTATCGAATTTATGACAAACAGGAGGGTAAAACAAAGAATCA
ATATCTTCAGGAGATGCTCCAAGAAGTTCTGGATTGGGGGCTCCGACCGACGACCTTCACTAGCGATGCTTGGTATGCGTCCAAGGCCAATCTCAATTTA
CTCAAAGACGTGCAAATGGGATTCCTGGTTGGTGTTGCCAAAAATCGACAGGTGCGAGTGGGCGCTCAGCAGTATCAACGAGTGGATAACCTCATTATTT
CAGAACAAGGATTACACGTTCATCTCAAAGGGGTGGGCATCGTCAAGTTTTTTTGCCAGCGGTTCAAAAACGGATCGTGTCGCTATTACCTTCTCTATGC
GCCAGACCCGAAAGAGCTAGCTTATGCTGGTAAAGCAGAGTTTGAGCACTTACATACCCTGCACTGGGGTATTGAATGCTTTCATCGTGCAGGTAAACAA
CTGTGTGGGCTTCAGCGATTTCGGGTGCGTTTGACGGAAGCTGTTCATACCCATGTTTTTTGCGCTCTGAGGGCTTTTGTAGAGCTTGAATTACAAGTCT
GGCACCAACAGATAGACAACTGGTATGCCCTCCAGCGTAATTTATATCAGGAGGTTGCGCGACAGTTCATTCTTGAACAACCACTGTTGGGATAGCTCTC
TTGAACATTGGGTGTAATTTCTGTCAATGCGTTTGTCCTG
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1023 bp340 aa731095+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

LRIPSKPSTAHCTQDLYVRYLLAQPKGDGCSHMAEILQDVSHDSINRFLLRERYEPKDLFDLLVGNGWVELTGGIVSADDTILEKLYSNPKKMDLLGYYW
SSKYSKPILGIPLITLYYSSPNGLRVPINYRIYDKQEGKTKNQYLQEMLQEVLDWGLRPTTFTSDAWYASKANLNLLKDVQMGFLVGVAKNRQVRVGAQQ
YQRVDNLIISEQGLHVHLKGVGIVKFFCQRFKNGSCRYYLLYAPDPKELAYAGKAEFEHLHTLHWGIECFHRAGKQLCGLQRFRVRLTEAVHTHVFCALR
AFVELELQVWHQQIDNWYALQRNLYQEVARQFILEQPLLG

 

Blast result :
Comments
ISAcma42 is 63% aa similar to ISAcma22.
ISAcma16 is inserted inside the ISAcma42.
ISAcam42 was reconstructed in silico by deletion of ISAcma16 sequence and one of the direct repeat generated by its insertion.
References
1] Swingley,W.D., Chen,M., Cheung,P.C., Conrad,A.L., Dejesa,L.C.,Hao,J., Honchak,B.M., Karbach,L.E., Kurdoglu,A., Lahiri,S.,Mastrian,S.D., Miyashita,H., Page,L., Ramakrishna,P., Satoh,S., Sattley,W.M., Shimada,Y., Taylor,H.L., Tomo,T., Tsuchiya,T., Wang,Z.T., Raymond,J., Mimuro,M., Blankenship,R.E. and Touchman,J.W. (2008) Proc. Natl. Acad. Sci. U.S.A.
2] ISfinder annotation (2009)