ISMex9
- Family IS110
- Group IS1111
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Methylobacterium extorquens | Methylobacterium extorquens AM1 |
DNA section
IS Length : 1383 bp
Ends
IR Length : 10
IRL : gatggggTATTCGGGGTGCCCGGCGCGGTGGAAGCGGCGGCATGATGGCG
IRR : ctcTATTCGGGGTTCATCGGTCAACTCCCCTGCGTGCTCATCCCTATCCA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TCGGGGGCTA | AACAAGTTTCC | 0 |
DNA sequence
GATGGGGTATTCGGGGTGCCCGGCGCGGTGGAAGCGGCGGCATGATGGCGCTTCGATCCAACCTCGGAGCTCCGCCATGGACCAGCAGCACCACGTCGGC
CTCGATGTCTCCGTGAAGGAGACAGCGATCTGCATCGTCGATCCCCACGGCAAGGTCGTGCACCGCGCCACCGTCGAGAGCCACCCCGAAGTGATCGGCC
GGCATCTGATCGATCTCGGCCATGCCTACGCGCGGGTCGGCCTGGAGGCGGGACCGCTCTCGCCGTGGCTCTACGCCGGCTTGGTCGAGGTCGGGCTGCC
GGCGATCTGCGTCGAGACCCGTCACATGCACGCCGCCCTCTCGGCGCGCATCAACAAGACCGACCGCAACGACGCCCTGGGCATCGCGCAGATGATGCGG
GTCGGGCTGTTCAAGCCGGTCCACGTCAAGACGCCGGCCAGCCAGCAGCGCCGCCTACTCCTGACCTCGCGCAAGCTCCTGCAGCGCAAGGCCTACGACA
TCGAGAGCGACCTGCGCGGCCAGCTGCGCAACTTCGGCCTCAAGGTCGGCGTCGTCGGGGCGGTGGGCTTCGAGCAGCGCGTGCGCGACCTCGTGCTCGA
TCTGCCCTTCGTCGCCGCCGTGGTCCTGCCGCTGTTGGAGGCGCGCGCGGCGCTTCGGACTCAACTCGCCAAGCTGCACAAGATGCTGCTGGAGCAGGTT
CGCACCGACCCAGTCTGCCGGCGATTGATGACCGCGCCCGGAGTGGGGCCGGTCGTGGCGCTGACCTACCGCACCTGCGTCGACAACCCGGCCCGGTTTG
GACGTTCGCAGTGCGTCGGGGCGCATTACGGTCTGACGCCGCGGCTCTACCAGTCCGGCGAGACGATGCGGGTCGGGCGGATCTCGAAGTGCGGGGATCG
GATGATGCGCGCCGCGCTCTACGAAGCAGCGCTCGTGCTGCTGACCGGCTCACGCGGCCGGTGGTCAGGGCTGAAGGTGTGGGGGTTGGCCGTCGCCAAG
CGCCGGGGCATGCAGAAGGCCCTCGTGGCCGTTGCCCGCAAGCTGGCGATCGTGTTGCACCGCATGTGGCGTGACGGGACGGACTTCCGTTGGTCGGCCG
CGGCCACGACAGCGGCCTGAGGTCCACCGACGCCACCAAGTAACATCGAGCTCCTCCGGCGTGGAGGAGGTCCCTGCGGGGACGACGGGAGCGGGTGAGA
CCGAGGAAACTCGAGCGCCGGTTTTGCCGAGCGCGCGGCTTAGATGGCTCCGCCCACTCCCTCTGATCCCATCATGAGGCGGCACGCGCCGACCTCGGAG
AGAAGCAAGAACTCCGCAGGACCGTTCACGCGCTGGATAGGGATGAGCACGCAGGGGAGTTGACCGATGAACCCCGAATAGAG
CTCGATGTCTCCGTGAAGGAGACAGCGATCTGCATCGTCGATCCCCACGGCAAGGTCGTGCACCGCGCCACCGTCGAGAGCCACCCCGAAGTGATCGGCC
GGCATCTGATCGATCTCGGCCATGCCTACGCGCGGGTCGGCCTGGAGGCGGGACCGCTCTCGCCGTGGCTCTACGCCGGCTTGGTCGAGGTCGGGCTGCC
GGCGATCTGCGTCGAGACCCGTCACATGCACGCCGCCCTCTCGGCGCGCATCAACAAGACCGACCGCAACGACGCCCTGGGCATCGCGCAGATGATGCGG
GTCGGGCTGTTCAAGCCGGTCCACGTCAAGACGCCGGCCAGCCAGCAGCGCCGCCTACTCCTGACCTCGCGCAAGCTCCTGCAGCGCAAGGCCTACGACA
TCGAGAGCGACCTGCGCGGCCAGCTGCGCAACTTCGGCCTCAAGGTCGGCGTCGTCGGGGCGGTGGGCTTCGAGCAGCGCGTGCGCGACCTCGTGCTCGA
TCTGCCCTTCGTCGCCGCCGTGGTCCTGCCGCTGTTGGAGGCGCGCGCGGCGCTTCGGACTCAACTCGCCAAGCTGCACAAGATGCTGCTGGAGCAGGTT
CGCACCGACCCAGTCTGCCGGCGATTGATGACCGCGCCCGGAGTGGGGCCGGTCGTGGCGCTGACCTACCGCACCTGCGTCGACAACCCGGCCCGGTTTG
GACGTTCGCAGTGCGTCGGGGCGCATTACGGTCTGACGCCGCGGCTCTACCAGTCCGGCGAGACGATGCGGGTCGGGCGGATCTCGAAGTGCGGGGATCG
GATGATGCGCGCCGCGCTCTACGAAGCAGCGCTCGTGCTGCTGACCGGCTCACGCGGCCGGTGGTCAGGGCTGAAGGTGTGGGGGTTGGCCGTCGCCAAG
CGCCGGGGCATGCAGAAGGCCCTCGTGGCCGTTGCCCGCAAGCTGGCGATCGTGTTGCACCGCATGTGGCGTGACGGGACGGACTTCCGTTGGTCGGCCG
CGGCCACGACAGCGGCCTGAGGTCCACCGACGCCACCAAGTAACATCGAGCTCCTCCGGCGTGGAGGAGGTCCCTGCGGGGACGACGGGAGCGGGTGAGA
CCGAGGAAACTCGAGCGCCGGTTTTGCCGAGCGCGCGGCTTAGATGGCTCCGCCCACTCCCTCTGATCCCATCATGAGGCGGCACGCGCCGACCTCGGAG
AGAAGCAAGAACTCCGCAGGACCGTTCACGCGCTGGATAGGGATGAGCACGCAGGGGAGTTGACCGATGAACCCCGAATAGAG
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1044 bp | 347 aa | 77 | 1120 | + | No |
Description :
ORF sequence :
MDQQHHVGLDVSVKETAICIVDPHGKVVHRATVESHPEVIGRHLIDLGHAYARVGLEAGPLSPWLYAGLVEVGLPAICVETRHMHAALSARINKTDRNDA
LGIAQMMRVGLFKPVHVKTPASQQRRLLLTSRKLLQRKAYDIESDLRGQLRNFGLKVGVVGAVGFEQRVRDLVLDLPFVAAVVLPLLEARAALRTQLAKL
HKMLLEQVRTDPVCRRLMTAPGVGPVVALTYRTCVDNPARFGRSQCVGAHYGLTPRLYQSGETMRVGRISKCGDRMMRAALYEAALVLLTGSRGRWSGLK
VWGLAVAKRRGMQKALVAVARKLAIVLHRMWRDGTDFRWSAAATTAA
LGIAQMMRVGLFKPVHVKTPASQQRRLLLTSRKLLQRKAYDIESDLRGQLRNFGLKVGVVGAVGFEQRVRDLVLDLPFVAAVVLPLLEARAALRTQLAKL
HKMLLEQVRTDPVCRRLMTAPGVGPVVALTYRTCVDNPARFGRSQCVGAHYGLTPRLYQSGETMRVGRISKCGDRMMRAALYEAALVLLTGSRGRWSGLK
VWGLAVAKRRGMQKALVAVARKLAIVLHRMWRDGTDFRWSAAATTAA
Blast result :
Comments
ISMex9 is 66% aa similar to ISGdi15.
The IR of this IS are not at its termini. In the IS sequence as given7 nt separate IRl from the left-hand end of the element and 3 nt separate IRr from the right-hand end.
The IR of this IS are not at its termini. In the IS sequence as given7 nt separate IRl from the left-hand end of the element and 3 nt separate IRr from the right-hand end.
References
[1] Stephane Vuilleumier et.al. Methylobacterium genome sequences: a reference blueprint to investigate microbial metabolism of C1 compounds from natural and industrial sources. (2009)PLoS ONE Submitted.