ISAcma41

  • Family IS630
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_009925 ND Acaryochloris marina
Acaryochloris marina MBIC11017
DNA section
IS Length : 1139 bp

Ends


IR Length : 14/28

IRL : CTGCGTACGACTTGCGGCTTAACATTGTAGTGGAGAGATTTGGCAGACTG
IRR : CTCTAGACGGTTAAGAGATTAACTTTCTGGAAAGACTGAAACTTGAGTGA

Insertion site


Left flankDirect repeatRight flankDR Length
ATCAGTGAGGTATATCCCTCTTTT4
CAAGATCAGTTATAGCCCTCTTTT4
TTAGGTAAAGTATATTTAAAAGAA4
AAAATCAAATTATAGCCCTCTTTT4
AAAAGAGGGTTATAACCCTCTTTT4

DNA sequence

CTGCGTACGACTTGCGGCTTAACATTGTAGTGGAGAGATTTGGCAGACTGGGAGTATCCCTCAACCGACTCCCCTACTTGCATGATTAAAATTGATTTCA
CCGCAGAGGAAATCCAACACCTCAATTACGAACGCTATCATCACCCTCACCCACGGGTGCAGCGACGGATGGAAGTGCTCTACTTGAAAAGTCAGGGTCT
TCCCCATGCCCAGATTTGTCAGCTTTGTCAGATTAGTCGTCCGACCTTAGCTAAAACCCTTCGTCTGTATCAACAAGGCGGAATTGAAGGGTTGAAAACC
CTGGAATACAAAGGTCAATCCAGTTCACTCAACGCTCATAGCGATAGTGTGAGAGCCTATTTTGAGCAACACCCGCCCCGCACCAGTGCAGAAGCCCAAG
CAGTCATTGAACAATTGACAGGTATTAAGCGTTCTCCAACCCAGATCAAAGCCTTCCTCAAACGCATTGGTTGTCGCTCTCGAAAAGTGGGGTATGTGCC
GGGAAAGTCGAGTCTGCCCGAGAAAATTGAGGAGCAAGAGCAGTTTCGACAAACTCGCCTTGAACCCTTATTAGAGGAAGCTCAACGCCAAGAGCGTCTT
GTCTTTTTTGTGGATGCTGCTCATTTTGTTCACCGGGCTTATCTGGGGTTTCTCTGGTGCCTTCGTCGGATCTTCATCCCTTCTCCTTCTGGCCGTCAAC
GATTCAATGTTCTGGGTGCACTCAACGCTGTCACCAAAGAAGTCACCAGTATCACCAACCACACCTACATCAACTCTCATAGCATGTGTCTGTTGCTAGC
CAAGCTGGCACTACTCGACCCAGTCATACCCATCAGCGTCATTTTAGACAATGCCCGATATCAAAAATGCCAGCTGGTGACTGACTTTGCTGAGATCGTC
GATATTGAGTTAGTCTATTTGCCCTCCTATTCACCCCATTTGAATTTGATTGAGCGGCTTTGGCGCTTTGTGCGCAAGGAATGTCTCTACTCGAAGTATT
ATTCGGATTTTCACTCTTTCAAAGGGGCCATTCAACAGTGCATTGACCAATGCAATACAGAGCATAAGGCGAAACTAACCAGCCTGCTCTCACTCAAGTT
TCAGTCTTTCCAGAAAGTTAATCTCTTAACCGTCTAGAG
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1098 bp365 aa401137+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MADWEYPSTDSPTCMIKIDFTAEEIQHLNYERYHHPHPRVQRRMEVLYLKSQGLPHAQICQLCQISRPTLAKTLRLYQQGGIEGLKTLEYKGQSSSLNAH
SDSVRAYFEQHPPRTSAEAQAVIEQLTGIKRSPTQIKAFLKRIGCRSRKVGYVPGKSSLPEKIEEQEQFRQTRLEPLLEEAQRQERLVFFVDAAHFVHRA
YLGFLWCLRRIFIPSPSGRQRFNVLGALNAVTKEVTSITNHTYINSHSMCLLLAKLALLDPVIPISVILDNARYQKCQLVTDFAEIVDIELVYLPSYSPH
LNLIERLWRFVRKECLYSKYYSDFHSFKGAIQQCIDQCNTEHKAKLTSLLSLKFQSFQKVNLLTV

 

Blast result :
Comments
ISAcma41 is 96% aa similar to ISAcma14.
There were 5 copies of ISAcma41 in this genome.
4 copies have a insertion of T in postion 409, generating a non programmed frameshift. The transposase is probably inactive.
References
1] Swingley,W.D., Chen,M., Cheung,P.C., Conrad,A.L., Dejesa,L.C.,Hao,J., Honchak,B.M., Karbach,L.E., Kurdoglu,A., Lahiri,S.,Mastrian,S.D., Miyashita,H., Page,L., Ramakrishna,P., Satoh,S., Sattley,W.M., Shimada,Y., Taylor,H.L., Tomo,T., Tsuchiya,T., Wang,Z.T., Raymond,J., Mimuro,M., Blankenship,R.E. and Touchman,J.W. (2008) Proc. Natl. Acad. Sci. U.S.A.
2] ISfinder annotation (2009)