ISMex40
- Family IS5
- Group IS1031
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_010172 | ND | Methylobacterium extorquens | Methylobacterium extorquens PA1 |
DNA section
IS Length : 918 bp
Ends
IR Length : 15/16
IRL : GAGCCCGTCCGATAAGGGTTGAGTTTGGGGTTGAGTGGCCGGGATGGCGA
IRR : GAGTCTGTCCGATAAGTCACGAAGCGCGTCCGAGGCGTCGGACGAGGATC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GTCGGATCAATGAC | TTA | GGCTTGCTCGCGCT | 3 |
| GAGGCTGAGGCTAC | TCA | GACACCTAAGGCCG | 3 |
DNA sequence
GAGCCCGTCCGATAAGGGTTGAGTTTGGGGTTGAGTGGCCGGGATGGCGATGCTTCCAGAAGGGTGCTGAAGCCTGACCTGGAAGTGCCATGTGGACCCC
GACCACCCGGCGGCAGCATAGCCGCTCGGGCCTGCGCTACGAAACCGACCTGACGGATGCCGAATGGGCTGTGGTCGAGCCGCTGATGCCCAAGCCTGCA
CCGTGCGGGCGTCCGCCGGTCTGGACGATGCGGGAGATCCTGAACGCGATCTTCTACGTGCTGCGCGGCGGGATCGCGTGGCGGCTGATCCCCAAGGATC
TGCCACCCCGCTCGACCACCTACGGCTACTTCAGCCGCTGGCGCGACGACGGCCTGTTCGGCCGGATCAACCACCACCTCGTCATGGCTGACCGTGAACG
GATCGGACGCGAGGCTTCGCCGTCGGCGGCGGTTCTGGATAGCCAGAGCATCAAGACCACCGAGAGCGGTGGGCCGCGCGGCTACGATGCCGGCAAGAAG
GTGAAGGGCCGTAAGCGCCAAGCTCTGGTCGACACGGACGGGCGTGCCCTCGTCCTCGATCCGCAGCCCGCCGACGTACAGGATCGCGACGGAGCGGTGC
CGGTGCTGCGTTTGTCGCGTCGCACCTTCCCGTTCATCACCAAGGCGTTCGCCGATGCGGGCTATGCCGGAGACCGACCCGCCACGGCCACGAGCATCAC
CGTCGCGATCGTGCGCAAGCCACCCGGGCAGGTCGGCTTCGCCGTTCAACCGCGCCGCTGGGTCGTCGAGCGCTTCTTCGCCTGGATCAGCCGCAACCGC
CGCCTCTGGAAGGATCCGGAGGCCACTCTCGCCTCGGCCCAAGCCTTCCTCTACGCCGCCGCTGCCATGATCCTCGTCCGACGCCTCGGACGCGCTTCGT
GACTTATCGGACAGACTC
GACCACCCGGCGGCAGCATAGCCGCTCGGGCCTGCGCTACGAAACCGACCTGACGGATGCCGAATGGGCTGTGGTCGAGCCGCTGATGCCCAAGCCTGCA
CCGTGCGGGCGTCCGCCGGTCTGGACGATGCGGGAGATCCTGAACGCGATCTTCTACGTGCTGCGCGGCGGGATCGCGTGGCGGCTGATCCCCAAGGATC
TGCCACCCCGCTCGACCACCTACGGCTACTTCAGCCGCTGGCGCGACGACGGCCTGTTCGGCCGGATCAACCACCACCTCGTCATGGCTGACCGTGAACG
GATCGGACGCGAGGCTTCGCCGTCGGCGGCGGTTCTGGATAGCCAGAGCATCAAGACCACCGAGAGCGGTGGGCCGCGCGGCTACGATGCCGGCAAGAAG
GTGAAGGGCCGTAAGCGCCAAGCTCTGGTCGACACGGACGGGCGTGCCCTCGTCCTCGATCCGCAGCCCGCCGACGTACAGGATCGCGACGGAGCGGTGC
CGGTGCTGCGTTTGTCGCGTCGCACCTTCCCGTTCATCACCAAGGCGTTCGCCGATGCGGGCTATGCCGGAGACCGACCCGCCACGGCCACGAGCATCAC
CGTCGCGATCGTGCGCAAGCCACCCGGGCAGGTCGGCTTCGCCGTTCAACCGCGCCGCTGGGTCGTCGAGCGCTTCTTCGCCTGGATCAGCCGCAACCGC
CGCCTCTGGAAGGATCCGGAGGCCACTCTCGCCTCGGCCCAAGCCTTCCTCTACGCCGCCGCTGCCATGATCCTCGTCCGACGCCTCGGACGCGCTTCGT
GACTTATCGGACAGACTC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 813 bp | 270 aa | 90 | 902 | + | No |
Chemistry : DDE
ORF sequence :
MWTPTTRRQHSRSGLRYETDLTDAEWAVVEPLMPKPAPCGRPPVWTMREILNAIFYVLRGGIAWRLIPKDLPPRSTTYGYFSRWRDDGLFGRINHHLVMA
DRERIGREASPSAAVLDSQSIKTTESGGPRGYDAGKKVKGRKRQALVDTDGRALVLDPQPADVQDRDGAVPVLRLSRRTFPFITKAFADAGYAGDRPATA
TSITVAIVRKPPGQVGFAVQPRRWVVERFFAWISRNRRLWKDPEATLASAQAFLYAAAAMILVRRLGRAS
DRERIGREASPSAAVLDSQSIKTTESGGPRGYDAGKKVKGRKRQALVDTDGRALVLDPQPADVQDRDGAVPVLRLSRRTFPFITKAFADAGYAGDRPATA
TSITVAIVRKPPGQVGFAVQPRRWVVERFFAWISRNRRLWKDPEATLASAQAFLYAAAAMILVRRLGRAS
Blast result :
Comments
ISMex40 is 66% aa similar to ISRm15.
References
1] Stephane Vuilleumier et.al. Methylobacterium genome sequences: a reference blueprint to investigate microbial metabolism of C1 compounds from natural and industrial sources. (2009) PLoS ONE Submitted.
2] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Glavina del Rio,T., Dalin,E., Tice,H., Pitluck,S., Saunders,E., Brettin,T., Bruce,D., Detter,J.C., Han,C., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Kim,E., Marx,C. and Richardson,P. (2007) Direct submission GenBank.
2] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Glavina del Rio,T., Dalin,E., Tice,H., Pitluck,S., Saunders,E., Brettin,T., Bruce,D., Detter,J.C., Han,C., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Kim,E., Marx,C. and Richardson,P. (2007) Direct submission GenBank.