ISfinder Mirror Site

ISMex15

Family IS5
Group IS427

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
	ND	Methylobacterium extorquens	Methylobacterium extorquens AM1 Methylobacterium dichloromethanicum DM4

DNA section

IS Length : 832 bp

Ends

IR Length : 15/16

IRL : GGCTCCGGACCCATAAGTTTGGCTTGGCGGGTGTGCCTGTGATTTCTTCC
IRR : GGCTCCGGACTCATAACCAGTAGCTGACGGTAGCGGCGATGCAGACGGCC

Insertion site

Left flank	Direct repeat	Right flank	DR Length
CAACGGATTT	TA	GGACTATGGA	2
TTTCGACCGC	TA	GGATCGTAGG	2
GCAGAGCGGGATAC	TA	ATACCCTAGCCTCT	2
GACGCTCCGC	TA	GTGCGTCGAG	2
TGCTTTGCCC	TA	AGATACCTGC	2
CCAACACCAC	TA	AGGCCTGCTA	2
AACACCGAGC	TA	GGCCGACCGA	2
ACTGTCATCG	TA	GTGCAGCGCC	2
GAGCGGTGCC	TA	GGGCTCGCCG	2
TCCTGACCGT	TA	GAGCGAGATC	2
AACACCGGAT	TA	GTTCTGCCCA	2

DNA sequence

GGCTCCGGACCCATAAGTTTGGCTTGGCGGGTGTGCCTGTGATTTCTTCCGGGTGAGGGAGGAGCCAATGGGCGAGTTCTGGTTGACCGACGAGCAGTTT
GCAAAGATCGCACCGCACCTTCCGACAGACACCCGGGGTAAGGAGCGCGTCGACGACCGACGTGTGATCAGCGGGATCATCCACGTGTTGAAGTCGGGCG
GGCGTTGGACGGACGCCCCGCGCGAGATCTACGGCCCGAAGAAGACCCTCTACAATCGCTTCGTGCGCTGGGCGGCCAAGGGGATCTGGAGGGATCTGTT
CGAGACCCTGGCCCGGGCCGGTGGGCCACCCTCCCAGGTGCTGATCGACTCCTCGGCCGTGAAGGCGCATCGCTGCGCCTCCGGTGGAAAGGGGGGGAGC
AAAGCCATGCCATCGGCCGCTCGCGGGGTGGACGCACCACCAAGATCCACGCGCTCACCGACCGGTTCTGCCGCCCGCTCGCCTTCCTGCTGACGGGCGG
TCAGGTGGCGGACTGCAAGGCGGGGGCACAGCTCTTGGAGGGCGCGCCAGCGTGCCGGATCATGTTGGCCGACAAGGGCTACGACAGCGACGCCATCCGC
CGTCAGATCGAGGCGACGGGGGCGGCGCCCAACATCCCGCCCAAGTCCAACCGGCGATGGAAGCCGTGCTTCTCCCCCGTCCTGTACAAGGGCCGCAACG
CCATCGAGCGGATGTTCGGGCGCCTCAAGGACTTCCGCCGCATCGCCACCCGATACGACCGGCTGGCAGCCAACTACCTGGCGGCCGTCTGCATCGCCGC
TACCGTCAGCTACTGGTTATGAGTCCGGAGCC

Recoding section

Recoding by frameshift
Frame
Type
Experimentally demonstrated

Stimulators :

Shine-Dalgarno sequence :
Secondary structure :

Recoding motif :

Protein section

ORF number : 3

ORF 1

Length		Begin	End	Strand	Fusion ORF
426 bp	141 aa	68	493	+	No

ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MGEFWLTDEQFAKIAPHLPTDTRGKERVDDRRVISGIIHVLKSGGRWTDAPREIYGPKKTLYNRFVRWAAKGIWRDLFETLARAGGPPSQVLIDSSAVKA
HRCASGGKGGSKAMPSAARGVDAPPRSTRSPTGSAARSPSC

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
555 bp	184 aa	268	822	+	No

ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

LGGQGDLEGSVRDPGPGRWATLPGADRLLGREGASLRLRWKGGEQSHAIGRSRGGRTTKIHALTDRFCRPLAFLLTGGQVADCKAGAQLLEGAPACRIML
ADKGYDSDAIRRQIEATGAAPNIPPKSNRRWKPCFSPVLYKGRNAIERMFGRLKDFRRIATRYDRLAANYLAAVCIAATVSYWL

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
755 bp	250 aa	68	822	+	Yes

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MGEFWLTDEQFAKIAPHLPTDTRGKERVDDRRVISGIIHVLKSGGRWTDAPREIYGPKKTLYNRFVRWAAKGIWRDLFETLARAGGPPSQVLIDSSAVKA
HRCASGGKGGQSHAIGRSRGGRTTKIHALTDRFCRPLAFLLTGGQVADCKAGAQLLEGAPACRIMLADKGYDSDAIRRQIEATGAAPNIPPKSNRRWKPC
FSPVLYKGRNAIERMFGRLKDFRRIATRYDRLAANYLAAVCIAATVSYWL

Blast result :

Comments

ISMex15 is 84% aa similar to ISSen2.
The third ORF is a putative ORFAB transposase reconstructed in silico by -1 programmed frameshift.

References

1] Stephane Vuilleumier et.al. Methylobacterium genome sequences: a reference blueprint to investigate microbial metabolism of C1 compounds from natural and industrial sources. (2009)PLoS ONE Submitted.