ISMdi7

  • Family IS21
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Methylobacterium dichloromethanicum
Methylobacterium extorquens AM1
Methylobacterium dichloromethanicum DM4
DNA section
IS Length : 2568 bp

Ends


IR Length : 10/12

IRL : TGTGTAGGGCGGTGACAAAACCATCCACTGGAGCGTCGGCTTTGTGCTGA
IRR : TGTAAAGGGCGGACCAATTTTAGGCCGCCGTGGCGGAGTAAAACCAGGCC

Insertion site


Left flankDirect repeatRight flankDR Length
GTTGTCGGAGCACCTGCGCG0
CTAATCGCGTCCCTTATCCT0

DNA sequence

TGTGTAGGGCGGTGACAAAACCATCCACTGGAGCGTCGGCTTTGTGCTGATGCGGGCGGTGTAAAAGCCGGCCAGTGGAGAGGCTTCTCTTTCGAGAGGA
GCCGAGGATGTTCGTCGTGGAAGTCTACGCAGCCGTTCGGCAGTTCGTGTTCATCGAGGGCCAGTCCCGGCGTGAGGCGGCTCGGGTCTTCGGGTTGAGC
CGAGAGACGATCGCCAAGATGTGTCGGTTCTCGTTGCCGCCGGGCTACACACGCTCGAAGCCGGTCGAGAAGCCGAAGCTCGGGCCTCTTCTGCCAGTGA
TCGCGGCGATCCTTGAGGCGGACCGGACTGCACCGCTCAAGCAACGCCATACGGCCAAGCGGATCTTCGAGCGCCTACGCGACGAGCACGGCTATGCCGG
TGGCTATACCGTGGTGAAGGACCACGTACGGATCTGCCGAGCACGGGGGCAGGAGACTTTCGTGCCGCTCGCCCACCCGCCCGGCCATGCCCAGGTCGAT
TTTGGCGAGGCGGTCGCCACCATCGCTGGCGTGCGCCGCAAGATCCATTTCTTCTGCATGGATCTGCCGCACTCCGACGCCTGCTTCGTGAAGGCGTATC
CGCGGGAGACCACCGAAGCTTTTCTCGACGGGCATGTCGCCGCCTTCGCCTTCTTCGGCGGCGTGCCTCTGTCGATCCTGTACGACAACACCAAGATCGC
AGTGGCCAAGATCTGCGGCGACGGACAGCGTGAGCGTACGCGCGCCTTCACCGAGTTGGTGAGCCACTGCCTGTTCCGGGATCGCTTCGGCCGTCCGGGC
AGGGGCAACGACAAGGGCAAGGTCGAGGGGCTGGTCAAGTTCGCCCGGTCCCACTTCATGACCCCGGCTCCGGAGGCGGCCTCGTTCGAGGCGCTGAACG
CTGACCTGGAGCGCCGCTGCCGAGCTCGGCAGAACGAGTGCGCCGGGCGGCATCCCGAGAGCATCGGAACGCGGCTCATGGCCGATCGAGTGGTTCTGCG
AGCCCTGCCGGCGGTGCCGCTGGAGCCGTGCGAGAAGAGGGCCGGGCGCGTCTCGTCGACCGCGCTGGTGCGCTATCGCGGCAACGACTACTCGGTGCCG
ACCACCTACGGCTTCCGGGACGTGCTGGTGAAGGGCTTCGTCGAGGAAGTCGTGATCCTGTGTGCGGGGGTCGAGATCGCCCGGCACCCGCGCAGCTACG
GCAGCGGCGTTTTCGTCGCCGAACCTCTGCACTACCTCGCGCTGATCGAGACCAAGCCGAACGCCCTCGACCAAGCCGCGGCACTCCAGGGCTGGGATCT
GCCCGAGGCGTTCCAGCACCTGCGCCACCTTCTGGAGGCGCGCATGGGCAACCGCGGCAAGCGCGAGTTCATCCAGGTGCTGCGCCTGATGGAGGCGATG
CCGAAGGACCTCGTGGCCTGGGCCGTCACCGAGGCGATCCGGCTCGGGGCGATTGGCTTCGATGCGGTCAAGTTGATCGCGCTGGCCCGTCTCGAACGGC
GGCCGCCTCGGCTCGACTTGTCGGCCTACCCGCATCTGCCCCGGCCTGCGGTGCGCGCGACGATGGCCGCCGACTACACGGTGCTGGTGCCGGAGGTGGC
GGCATGAGCGTGAGCGGCGATGAGACGACACCGGGCGTCCTGCTCGCCCATCACCTCAAGCAGTTGAAGTTGCCGACGGTCCTGCGCGAGTACGACAAGG
TCGCCCGGGACTGCGCCCGGAGCGGCCTCGACCACCCCCGCTATCTGCTGCGGCTGGTTGAGCTGGAACTGATCGACCGCGAACGGCGCATGGTCGAGCG
CCGGATCCGGGCGGCACGCTTCCCGGCGGTGAAGAGCCTCGACACCTTCGACTTCGCCGCCATCCCGAGCCTGAACAAGATGCTCGTGCTGGAGCTGGCG
CGCTGCGGCTACGTCCTCGGTCGGGAGAACGTCATCGCGCTCGGCAACTCCGGCACCGGCAAGACGCACATCGCCCTGGCTCTCGGACTGGCAGCCTGCC
AGAAGGGCTTCTCGGTCACGTTCACCACGGCGGCCTCGCTGGTCAACCAGCTCATGGAGGCGCGTGACGAGCGCCGCCTGCTCCGGCTTCAGAGGGAGCT
GGCCGCGGTGAAGTTGCTGATTGTCGATGAACTCGGCTACGTGCCGCTGTCGCCGACGGGGGCCGAGCTTTTGTTCGAGGTCCTGTCCCAGCGCTACGAG
CGCGGCTCGACGGTGGTGACCTCGAACCTTCCGTTCGAGGACTGGACCTCGGTCCTGGGCTCGGAGCGGCTGACCGGTGCGCTGCTGGATCGGCTGACCC
ATCACGTCAGCATCCTGAGCCTGAACGGAGACAGCTACCGCCTCAGATCCTCCCGCAGCCGGCGGGGCCGCACAGACAGGGCGGAGCAAAACCAGGCCAC
CCTTGATGACCCTGATCCCGCGACGGGCGAGATCCGGCCAGCCTGACCTCACGATCCGCGACAAACGATGAAGAGGCCCGATCGGGCCCCTTCATCGTTC
AGGCTGTCCGCAGTCCGTGGCCTGGTTTTACTCCGCCACGGCGGCCTAAAATTGGTCCGCCCTTTACA
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
1500 bp499 aa1081607+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MFVVEVYAAVRQFVFIEGQSRREAARVFGLSRETIAKMCRFSLPPGYTRSKPVEKPKLGPLLPVIAAILEADRTAPLKQRHTAKRIFERLRDEHGYAGGY
TVVKDHVRICRARGQETFVPLAHPPGHAQVDFGEAVATIAGVRRKIHFFCMDLPHSDACFVKAYPRETTEAFLDGHVAAFAFFGGVPLSILYDNTKIAVA
KICGDGQRERTRAFTELVSHCLFRDRFGRPGRGNDKGKVEGLVKFARSHFMTPAPEAASFEALNADLERRCRARQNECAGRHPESIGTRLMADRVVLRAL
PAVPLEPCEKRAGRVSSTALVRYRGNDYSVPTTYGFRDVLVKGFVEEVVILCAGVEIARHPRSYGSGVFVAEPLHYLALIETKPNALDQAAALQGWDLPE
AFQHLRHLLEARMGNRGKREFIQVLRLMEAMPKDLVAWAVTEAIRLGAIGFDAVKLIALARLERRPPRLDLSAYPHLPRPAVRATMAADYTVLVPEVAA

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
843 bp280 aa16042446+No
ORF function : Accessory Gene
AG : IS21 helper

ORF sequence :

MSVSGDETTPGVLLAHHLKQLKLPTVLREYDKVARDCARSGLDHPRYLLRLVELELIDRERRMVERRIRAARFPAVKSLDTFDFAAIPSLNKMLVLELAR
CGYVLGRENVIALGNSGTGKTHIALALGLAACQKGFSVTFTTAASLVNQLMEARDERRLLRLQRELAAVKLLIVDELGYVPLSPTGAELLFEVLSQRYER
GSTVVTSNLPFEDWTSVLGSERLTGALLDRLTHHVSILSLNGDSYRLRSSRSRRGRTDRAEQNQATLDDPDPATGEIRPA

 

Blast result :
Comments
ISMdi7 has 2 intact copies in DM4 and 1 partial copy in AM1.
ISMdi7 is 94% and 92% aa similar to orfA and orfB of ISMex8.
References