ISMex29
- Family IS110
- Group IS1111
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Methylobacterium dichloromethanicum | Methylobacterium extorquens AM1 Methylobacterium dichloromethanicum DM4 |
DNA section
IS Length : 1339 bp
Ends
IR Length : 13
IRL : tgatggtGTGGATGGCTCCTGCTCCCGGCGTTGCGTTGGGGCATAGTCGG
IRR : -----GTATGGATGGCTCCTGCGGGTCAAGGATTTCGATCGGTTTGGGTG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GCTCGAT | ACGGGTTGAAGTC | 0 | |
| AGACGAGTTCGAT | TTGAAGTCGGGTGA | 0 |
DNA sequence
TGATGGTGTGGATGGCTCCTGCTCCCGGCGTTGCGTTGGGGCATAGTCGGGATGTTGTCGCCTCAAGCTGAGGAGCCGCCCATGTCCGAGCTTGCTACGG
TCGGTATCGACCTCGCGAAGAGCGTTTTCCAAATCCACGGTGTCGATGAAAACGGTCGTGTTCTCGTGCGTCGCCAACTGCGTCGAGGTCAGTTGCTCGC
GTTCTTTGAGAAGCTGCCGCGGTGCCTGATCGGCATGGAGGCCTGCGGGGGCGCTCACGACTGGGCGCGCAAGCTTCAGGAACTGGGGCATGACGTCCGG
CTGATGCCGCCGTCTTACGTGAAGCCCTACGTGAAGCGCGGGAAGACGGACGCAGCCGACGCGGCGGCCATCTGTGAAGCGGTCACGCGTCCCTCGATGC
GGTTCGTCGCCGTGAAGAGCGAAGCTTGCTCTTCGGTCCTGGTCGTCCACCGCACGCGGGATCTTCTGGTTCGCCAGCGCACGCAGATCTGCAACGCGAT
CCGCGCCCACATGACAGAGTTCGGCGTCATTGCAGCGAAGGGCGCGCAGAACGTCGATCGGCTGAGGACGCAACTGCATCAAATGCCGGAAGCGGCCCGA
TTGCCCGTCAGCCTCCTCTTCGAGCAGTTGGTCGATACCGACACGCGGATCGAGCGGTTGACGCGTGAGATCGAAGCGGCCCACGCCCAAAGCGAGACGA
GCCAGCGTCTGGTGACGATCCCGGGCGTCGGCAAGCTCTCGGCGACGATCATCGCGGCGACGACGCCGGACGTCGGCAACTTCGACTGCGCGAGGGACTA
CGCCGCCTGGCTCGGCCTGACCCCCAAACCGCATTCCACCGGCGGCAAACAGAGGATCGGGCGGATCTCGAAGATGGGCAACCGGTATATTCGGCGGTTG
CTCTATCTGGGCGCCATGTCGCAGATCATGGTGAGACACAGGTGCGGCCCGAGACAGGACTGGCTGTCCGACATGCTGGCCCGAAAGAAGACGAAAGTCG
CCGCCATCGCATTGGCGCATCGCATGGCAAGAACGATCTTTGCCGTCCTCAGAGACGGATCACGCTACCAGCCGCTGGCGACTGCTGCCTGAACGGCTGA
ACGAACGGTCAGGGCGACGTGAGGTGATGGCGAACGAACGGAGAGGAAGAACCAGGACACCCCGATCAATCCGAAGCGCTCTGAGCGCGCTCGATTGAGT
GGGACCTGTTCTCCAAGCGGACTTCATCAGGGCGCGCAGCGATCCCGCTGCAGATCAGACGCCGTATACATGGCCGCAGCCGACCAGTTCACCCAAACCG
ATCGAAATCCTTGACCCGCAGGAGCCATCCATAC
TCGGTATCGACCTCGCGAAGAGCGTTTTCCAAATCCACGGTGTCGATGAAAACGGTCGTGTTCTCGTGCGTCGCCAACTGCGTCGAGGTCAGTTGCTCGC
GTTCTTTGAGAAGCTGCCGCGGTGCCTGATCGGCATGGAGGCCTGCGGGGGCGCTCACGACTGGGCGCGCAAGCTTCAGGAACTGGGGCATGACGTCCGG
CTGATGCCGCCGTCTTACGTGAAGCCCTACGTGAAGCGCGGGAAGACGGACGCAGCCGACGCGGCGGCCATCTGTGAAGCGGTCACGCGTCCCTCGATGC
GGTTCGTCGCCGTGAAGAGCGAAGCTTGCTCTTCGGTCCTGGTCGTCCACCGCACGCGGGATCTTCTGGTTCGCCAGCGCACGCAGATCTGCAACGCGAT
CCGCGCCCACATGACAGAGTTCGGCGTCATTGCAGCGAAGGGCGCGCAGAACGTCGATCGGCTGAGGACGCAACTGCATCAAATGCCGGAAGCGGCCCGA
TTGCCCGTCAGCCTCCTCTTCGAGCAGTTGGTCGATACCGACACGCGGATCGAGCGGTTGACGCGTGAGATCGAAGCGGCCCACGCCCAAAGCGAGACGA
GCCAGCGTCTGGTGACGATCCCGGGCGTCGGCAAGCTCTCGGCGACGATCATCGCGGCGACGACGCCGGACGTCGGCAACTTCGACTGCGCGAGGGACTA
CGCCGCCTGGCTCGGCCTGACCCCCAAACCGCATTCCACCGGCGGCAAACAGAGGATCGGGCGGATCTCGAAGATGGGCAACCGGTATATTCGGCGGTTG
CTCTATCTGGGCGCCATGTCGCAGATCATGGTGAGACACAGGTGCGGCCCGAGACAGGACTGGCTGTCCGACATGCTGGCCCGAAAGAAGACGAAAGTCG
CCGCCATCGCATTGGCGCATCGCATGGCAAGAACGATCTTTGCCGTCCTCAGAGACGGATCACGCTACCAGCCGCTGGCGACTGCTGCCTGAACGGCTGA
ACGAACGGTCAGGGCGACGTGAGGTGATGGCGAACGAACGGAGAGGAAGAACCAGGACACCCCGATCAATCCGAAGCGCTCTGAGCGCGCTCGATTGAGT
GGGACCTGTTCTCCAAGCGGACTTCATCAGGGCGCGCAGCGATCCCGCTGCAGATCAGACGCCGTATACATGGCCGCAGCCGACCAGTTCACCCAAACCG
ATCGAAATCCTTGACCCGCAGGAGCCATCCATAC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1011 bp | 336 aa | 82 | 1092 | + | No |
Chemistry : DEDD
ORF sequence :
MSELATVGIDLAKSVFQIHGVDENGRVLVRRQLRRGQLLAFFEKLPRCLIGMEACGGAHDWARKLQELGHDVRLMPPSYVKPYVKRGKTDAADAAAICEA
VTRPSMRFVAVKSEACSSVLVVHRTRDLLVRQRTQICNAIRAHMTEFGVIAAKGAQNVDRLRTQLHQMPEAARLPVSLLFEQLVDTDTRIERLTREIEAA
HAQSETSQRLVTIPGVGKLSATIIAATTPDVGNFDCARDYAAWLGLTPKPHSTGGKQRIGRISKMGNRYIRRLLYLGAMSQIMVRHRCGPRQDWLSDMLA
RKKTKVAAIALAHRMARTIFAVLRDGSRYQPLATAA
VTRPSMRFVAVKSEACSSVLVVHRTRDLLVRQRTQICNAIRAHMTEFGVIAAKGAQNVDRLRTQLHQMPEAARLPVSLLFEQLVDTDTRIERLTREIEAA
HAQSETSQRLVTIPGVGKLSATIIAATTPDVGNFDCARDYAAWLGLTPKPHSTGGKQRIGRISKMGNRYIRRLLYLGAMSQIMVRHRCGPRQDWLSDMLA
RKKTKVAAIALAHRMARTIFAVLRDGSRYQPLATAA
Blast result :
Comments
ISMex29 is 69% aa similar to ISMmg2.
IR are not at the termini, in the IS sequence as given 7 nt separate IRl from the left-hand end of the element and 3 nt separate IRr from the right-hand end.
IR are not at the termini, in the IS sequence as given 7 nt separate IRl from the left-hand end of the element and 3 nt separate IRr from the right-hand end.
References
1] Stephane Vuilleumier et.al. Methylobacterium genome sequences: a reference blueprint to investigate microbial metabolism of C1 compounds from natural and industrial sources. (2009) PLoS ONE Submitted.