ISAnsp8

  • Family IS3
  • Group IS407
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_011145 ND Anaeromyxobacter sp.
Anaeromyxobacter sp. K
DNA section
IS Length : 1225 bp

Ends


IR Length : 22/26

IRL : TGACGTCCCCCCGGAAAACTGGACGGAAGATGACTTTAGGACTTCGCTGG
IRR : TGACGTCCCCCCAGATCCTGGACGGTTTCAAACCTCAGGAAACTTCACTG

Insertion site


Left flankDirect repeatRight flankDR Length
GGCGGACGTGAACGGTCTCTGCTGAACCG0

DNA sequence

TGACGTCCCCCCGGAAAACTGGACGGAAGATGACTTTAGGACTTCGCTGGGCTGAGACCCTGAGGAAAGGGGATCGCCGATGCGGAAGTCGAGGTTCACC
GAGGAGCAGATTGCGCACGCGCTGCGCCAGGTCGACGCAGGCGTGCCGGCCGCCGAACTCTGCCGGAAGCTCGGCATCAGCGAGCAGACGTTCTACGCCT
GGAAGAAGAAGTACGCGGGGATGGGCATCGCCGAGATGCGCCGGGTGAAGCAGCTCGAGGACGAGAACCGGCGCCTGAAGACCCTCGTCGCGGACCTCAC
CCTGGACAAGCACATGCTCCAGGAGGTCCTTCGAAAAAAATTCTAAGGCCCGCGGCGTTGCGACAACGCGCAGATTGGCTGGTGGGAGCTTTCCGCATCA
GCAAGCTGCGGGCCTGCAAGCTCGTTCGGCTTGGCCGGTCGACGTACTACTGGCGGAGCACGGCGAAGGACCAGACGCCTCTGCGGACGCGCCTTCGCGA
ACTCGCCGAGGCGCGGCCGCGATTCGGCTACCCGCGGCTGCACGTGATGCTCCGCCGGGAGGGCTGGAAGGTGAACATCAAACGCGTCTACCGCCTCTAC
AAGCTGGAAGGGTTGAACGTCAGGTACAAGAAGCGGAAGAAGCGCGCGAGCCACCTGCGCGTCGTGCCGCCCGCGCCGACGGCGGCAAACGAGCGCTGGA
GCATGGACTTCATGCGGGACACCCTCGATGACGGCCGGCCATTCCGGATCTTCACGCTCGTCGACGTCTTCACTCGCGAGTGCCCGCTCCTCGCCGCCGA
CACCTCGCTCACCGGGCACAAGGTCGCCGAGTTGCTCGACCACGTCGCAGTTGAGCGCGGATACCCGAAGACGATCACCGTGGACAACGGGACCGAGTTC
TACTCGAAGGCCATGGACTCCTGGGCCTACCGCAACGGCGTCCAGCTGCAGTTCATCCGGCCGGGCAAGCCCGTCGAGAATGCCTTCATCGAGAGCTTCA
ACGGCAGGCTCCGAGACGAGCTGTTGAACAGCGAGCTCTTCATGGGGTTACATGATGCTCGCCAGAAGCTGGAGACTTGGCGGCTGGACTACAACCAGAA
CCGCCCTCACAGCGCGATCGGACACCTGACGCCGGTCGAGTTCGCCAACCAGGTCAGGACGGAGGCTCATGCCCTCAGTGAAGTTTCCTGAGGTTTGAAA
CCGTCCAGGATCTGGGGGGACGTCA
Recoding section
  • Recoding by frameshift
  • Frame
  • Type
  • Experimentally demonstrated

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
267 bp88 aa80346+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MRKSRFTEEQIAHALRQVDAGVPAAELCRKLGISEQTFYAWKKKYAGMGIAEMRRVKQLEDENRRLKTLVADLTLDKHMLQEVLRKKF

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
951 bp316 aa2411191+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

PGEAARGREPAPEDPRRGPHPGQAHAPGGPSKKILRPAALRQRADWLMGAFRISKLRACKLVRLGRSTYYWRSTAKDQTPLRTRLRELAEARPRFGYPRL
HVMLRREGWKVNIKRVYRLYKLEGLNVRYKKRKKRASHLRVVPPAPTAANERWSMDFMRDTLDDGRPFRIFTLVDVFTRECPLLAADTSLTGHKVAELLD
HVAVERGYPKTITVDNGTEFYSKAMDSWAYRNGVQLQFIRPGKPVENAFIESFNGRLRDELLNSELFMGLHDARQKLETWRLDYNQNRPHSAIGHLTPVE
FANQVRTEAHALSEVS

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1112 bp370 aa801191+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MRKSRFTEEQIAHALRQVDAGVPAAELCRKLGISEQTFYAWKKKYAGMGIAEMRRVKQLEDENRRLKTLVADLTLDKHMLQEVLRKKILRPAALRQRADW
LMGAFRISKLRACKLVRLGRSTYYWRSTAKDQTPLRTRLRELAEARPRFGYPRLHVMLRREGWKVNIKRVYRLYKLEGLNVRYKKRKKRASHLRVVPPAP
TAANERWSMDFMRDTLDDGRPFRIFTLVDVFTRECPLLAADTSLTGHKVAELLDHVAVERGYPKTITVDNGTEFYSKAMDSWAYRNGVQLQFIRPGKPVE
NAFIESFNGRLRDELLNSELFMGLHDARQKLETWRLDYNQNRPHSAIGHLTPVEFANQVRTEAHALSEVS

 

Blast result :
Comments
ISAnsp8 is 74% aa similar to ISGme7. The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] ISfinder annotation (2009)
2] Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Saunders,E., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Kyrpides,N.,Ovchinnikiva,G. and Beliaev,A. (2008) Direct submission GenBank