ISfinder Mirror Site

ISAnsp8

Family IS3
Group IS407

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_011145	ND	Anaeromyxobacter sp.	Anaeromyxobacter sp. K

DNA section

IS Length : 1225 bp

Ends

IR Length : 22/26

IRL : TGACGTCCCCCCGGAAAACTGGACGGAAGATGACTTTAGGACTTCGCTGG
IRR : TGACGTCCCCCCAGATCCTGGACGGTTTCAAACCTCAGGAAACTTCACTG

Insertion site

Left flank	Direct repeat	Right flank	DR Length
GGCGGACGTGAACGGT		CTCTGCTGAACCG	0

DNA sequence

TGACGTCCCCCCGGAAAACTGGACGGAAGATGACTTTAGGACTTCGCTGGGCTGAGACCCTGAGGAAAGGGGATCGCCGATGCGGAAGTCGAGGTTCACC
GAGGAGCAGATTGCGCACGCGCTGCGCCAGGTCGACGCAGGCGTGCCGGCCGCCGAACTCTGCCGGAAGCTCGGCATCAGCGAGCAGACGTTCTACGCCT
GGAAGAAGAAGTACGCGGGGATGGGCATCGCCGAGATGCGCCGGGTGAAGCAGCTCGAGGACGAGAACCGGCGCCTGAAGACCCTCGTCGCGGACCTCAC
CCTGGACAAGCACATGCTCCAGGAGGTCCTTCGAAAAAAATTCTAAGGCCCGCGGCGTTGCGACAACGCGCAGATTGGCTGGTGGGAGCTTTCCGCATCA
GCAAGCTGCGGGCCTGCAAGCTCGTTCGGCTTGGCCGGTCGACGTACTACTGGCGGAGCACGGCGAAGGACCAGACGCCTCTGCGGACGCGCCTTCGCGA
ACTCGCCGAGGCGCGGCCGCGATTCGGCTACCCGCGGCTGCACGTGATGCTCCGCCGGGAGGGCTGGAAGGTGAACATCAAACGCGTCTACCGCCTCTAC
AAGCTGGAAGGGTTGAACGTCAGGTACAAGAAGCGGAAGAAGCGCGCGAGCCACCTGCGCGTCGTGCCGCCCGCGCCGACGGCGGCAAACGAGCGCTGGA
GCATGGACTTCATGCGGGACACCCTCGATGACGGCCGGCCATTCCGGATCTTCACGCTCGTCGACGTCTTCACTCGCGAGTGCCCGCTCCTCGCCGCCGA
CACCTCGCTCACCGGGCACAAGGTCGCCGAGTTGCTCGACCACGTCGCAGTTGAGCGCGGATACCCGAAGACGATCACCGTGGACAACGGGACCGAGTTC
TACTCGAAGGCCATGGACTCCTGGGCCTACCGCAACGGCGTCCAGCTGCAGTTCATCCGGCCGGGCAAGCCCGTCGAGAATGCCTTCATCGAGAGCTTCA
ACGGCAGGCTCCGAGACGAGCTGTTGAACAGCGAGCTCTTCATGGGGTTACATGATGCTCGCCAGAAGCTGGAGACTTGGCGGCTGGACTACAACCAGAA
CCGCCCTCACAGCGCGATCGGACACCTGACGCCGGTCGAGTTCGCCAACCAGGTCAGGACGGAGGCTCATGCCCTCAGTGAAGTTTCCTGAGGTTTGAAA
CCGTCCAGGATCTGGGGGGACGTCA

Recoding section

Recoding by frameshift
Frame
Type
Experimentally demonstrated

Stimulators :

Shine-Dalgarno sequence :
Secondary structure :

Recoding motif :

Protein section

ORF number : 3

ORF 1

Length		Begin	End	Strand	Fusion ORF
267 bp	88 aa	80	346	+	No

ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MRKSRFTEEQIAHALRQVDAGVPAAELCRKLGISEQTFYAWKKKYAGMGIAEMRRVKQLEDENRRLKTLVADLTLDKHMLQEVLRKKF

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
951 bp	316 aa	241	1191	+	No

ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

PGEAARGREPAPEDPRRGPHPGQAHAPGGPSKKILRPAALRQRADWLMGAFRISKLRACKLVRLGRSTYYWRSTAKDQTPLRTRLRELAEARPRFGYPRL
HVMLRREGWKVNIKRVYRLYKLEGLNVRYKKRKKRASHLRVVPPAPTAANERWSMDFMRDTLDDGRPFRIFTLVDVFTRECPLLAADTSLTGHKVAELLD
HVAVERGYPKTITVDNGTEFYSKAMDSWAYRNGVQLQFIRPGKPVENAFIESFNGRLRDELLNSELFMGLHDARQKLETWRLDYNQNRPHSAIGHLTPVE
FANQVRTEAHALSEVS

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
1112 bp	370 aa	80	1191	+	Yes

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MRKSRFTEEQIAHALRQVDAGVPAAELCRKLGISEQTFYAWKKKYAGMGIAEMRRVKQLEDENRRLKTLVADLTLDKHMLQEVLRKKILRPAALRQRADW
LMGAFRISKLRACKLVRLGRSTYYWRSTAKDQTPLRTRLRELAEARPRFGYPRLHVMLRREGWKVNIKRVYRLYKLEGLNVRYKKRKKRASHLRVVPPAP
TAANERWSMDFMRDTLDDGRPFRIFTLVDVFTRECPLLAADTSLTGHKVAELLDHVAVERGYPKTITVDNGTEFYSKAMDSWAYRNGVQLQFIRPGKPVE
NAFIESFNGRLRDELLNSELFMGLHDARQKLETWRLDYNQNRPHSAIGHLTPVEFANQVRTEAHALSEVS

Blast result :

Comments

ISAnsp8 is 74% aa similar to ISGme7. The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.

References

1] ISfinder annotation (2009)
2] Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Saunders,E., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Kyrpides,N.,Ovchinnikiva,G. and Beliaev,A. (2008) Direct submission GenBank