ISAae1
- Family IS5
- Group IS903
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_000918 | ND | Aquifex aeolicus | Aquifex aeolicus VF5 plasmid ece1 Aquifex aeolicus VF5 |
DNA section
IS Length : 1010 bp
Ends
IR Length : 16/20
IRL : AAGCCCGTTTAAAAAATCTTGACACAGTGGAAGAGAAGAAGTAGAAGGAA
IRR : GAGCCTGTTGAAAAAATCGTCATGACTTAGAGAATAAAGCGAGAGGAAGC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| AAACTTCGTT | AATTATAGT | TGACATTTCT | 9 |
| TTCTTCAACT | ACCGGGTAT | TGACTAACAA | 9 |
| AGGAATTGTA | AACCAAGGT | TTACAAATTT | 9 |
DNA sequence
AAGCCCGTTTAAAAAATCTTGACACAGTGGAAGAGAAGAAGTAGAAGGAAGGGAATGAGAACAAGAAATAACAAGCAATTGATAAAACTGATATTAACCA
AAAGCAGGGAAATATGCAATACCATTAGACCAAGTCAGTACAACAAAAGAGGAAGACCCAGAGTTTACGAAGATCACCTCATAGTAGCAGCACTACTGAT
TAAGATCTTAGAAAACCTCTCCCTCCGAGATTTAGAAGAAAGACTAAAAGACCTATTCCCCAAAGTACCTGACTTTACCACTCTCCACTACAGGTTCAGA
AAACTAAACCGGGGCTATCTCAAAGAGCTAATACACAGGACCGCAAAGGAGATAATGGAAAAGCTTCAGGCAAAAGAGTTTTACTGCCTTATTGCTGACG
GAACCGGATTTGGATATGCACAGGCATATGAACTCAAGTGGAAGAAGGGAAAGGAACTTAGGAATGTAAAATCTCATGTGAAAACAGAAGTTCTTGTAGG
AGTTGTAAGAAACAAAGCTATAGTAGTGGATATAAACACAGGCAAATCCTATGCAGATGAGAATATGCTGCTAAGATCCATGCTCAAAGAACTTGGCTTT
AGAGCGAGGTATTTTCTTGGGGATGCCTACTATGGGAAGTCTGCAGGAGTTTTAGAGGAAATAAAGAAGCTACGGATGGAGTCTATAGTTCCGGTGAGGG
ATACTGCACATACAAGAGTTAGGAACATGTATAGGCTATGGGCTAAGAGGAATTATGAGATAAGGAGGAAGGTTTATAGAAAGAACAGGTACAGGGTTGA
GCAGGTGATAGGGATAGTTAAGAACAGGTTTGGGGACAGGGACAATGTTTATGACTTTGAGATTGCGAGTTTGTATGTTCTAGGGAGGTTTGTTCTTTAC
AACTTGATCCTTCTTCTGAAGCTCCTTTTATTGTGTCTTAACCTTTTGAGGATTACATGGGCTTCCTCTCGCTTTATTCTCTAAGTCATGACGATTTTTT
CAACAGGCTC
AAAGCAGGGAAATATGCAATACCATTAGACCAAGTCAGTACAACAAAAGAGGAAGACCCAGAGTTTACGAAGATCACCTCATAGTAGCAGCACTACTGAT
TAAGATCTTAGAAAACCTCTCCCTCCGAGATTTAGAAGAAAGACTAAAAGACCTATTCCCCAAAGTACCTGACTTTACCACTCTCCACTACAGGTTCAGA
AAACTAAACCGGGGCTATCTCAAAGAGCTAATACACAGGACCGCAAAGGAGATAATGGAAAAGCTTCAGGCAAAAGAGTTTTACTGCCTTATTGCTGACG
GAACCGGATTTGGATATGCACAGGCATATGAACTCAAGTGGAAGAAGGGAAAGGAACTTAGGAATGTAAAATCTCATGTGAAAACAGAAGTTCTTGTAGG
AGTTGTAAGAAACAAAGCTATAGTAGTGGATATAAACACAGGCAAATCCTATGCAGATGAGAATATGCTGCTAAGATCCATGCTCAAAGAACTTGGCTTT
AGAGCGAGGTATTTTCTTGGGGATGCCTACTATGGGAAGTCTGCAGGAGTTTTAGAGGAAATAAAGAAGCTACGGATGGAGTCTATAGTTCCGGTGAGGG
ATACTGCACATACAAGAGTTAGGAACATGTATAGGCTATGGGCTAAGAGGAATTATGAGATAAGGAGGAAGGTTTATAGAAAGAACAGGTACAGGGTTGA
GCAGGTGATAGGGATAGTTAAGAACAGGTTTGGGGACAGGGACAATGTTTATGACTTTGAGATTGCGAGTTTGTATGTTCTAGGGAGGTTTGTTCTTTAC
AACTTGATCCTTCTTCTGAAGCTCCTTTTATTGTGTCTTAACCTTTTGAGGATTACATGGGCTTCCTCTCGCTTTATTCTCTAAGTCATGACGATTTTTT
CAACAGGCTC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 966 bp | 321 aa | 19 | 984 | + | No |
Chemistry : DDE
ORF sequence :
LTQWKRRSRRKGMRTRNNKQLIKLILTKSREICNTIRPSQYNKRGRPRVYEDHLIVAALLIKILENLSLRDLEERLKDLFPKVPDFTTLHYRFRKLNRGY
LKELIHRTAKEIMEKLQAKEFYCLIADGTGFGYAQAYELKWKKGKELRNVKSHVKTEVLVGVVRNKAIVVDINTGKSYADENMLLRSMLKELGFRARYFL
GDAYYGKSAGVLEEIKKLRMESIVPVRDTAHTRVRNMYRLWAKRNYEIRRKVYRKNRYRVEQVIGIVKNRFGDRDNVYDFEIASLYVLGRFVLYNLILLL
KLLLLCLNLLRITWASSRFIL
LKELIHRTAKEIMEKLQAKEFYCLIADGTGFGYAQAYELKWKKGKELRNVKSHVKTEVLVGVVRNKAIVVDINTGKSYADENMLLRSMLKELGFRARYFL
GDAYYGKSAGVLEEIKKLRMESIVPVRDTAHTRVRNMYRLWAKRNYEIRRKVYRKNRYRVEQVIGIVKNRFGDRDNVYDFEIASLYVLGRFVLYNLILLL
KLLLLCLNLLRITWASSRFIL
Blast result :
Comments
ISAae1 is 42% aa similar to ISSod12.
This IS has atypic IRs: it begins with 'AA' instead of 'GG'.
This IS has atypic IRs: it begins with 'AA' instead of 'GG'.
References
1] ISfinder annotation (2010)
2] Deckert,G., Warren,P.V., Gaasterland,T., Young,W.G., Lenox,A.L., Graham,D.E., Overbeek,R., Snead,M.A., Keller,M., Aujay,M., Huber,R., Feldman,R.A., Short,J.M., Olson,G.J. and Swanson,R.V. (1998) Nature 392 (6674), 353-358
3] Deckert,G., Warren,P.V., Gaasterland,T., Young,W.G., Lenox,A.L., Graham,D.E., Overbeek,R., Snead,M.A., Keller,M., Aujay,M., Huber,R., Feldman,R.A., Short,J.M., Olson,G.J. and Swanson,R.V.
Direct submission GenBank (1997)
2] Deckert,G., Warren,P.V., Gaasterland,T., Young,W.G., Lenox,A.L., Graham,D.E., Overbeek,R., Snead,M.A., Keller,M., Aujay,M., Huber,R., Feldman,R.A., Short,J.M., Olson,G.J. and Swanson,R.V. (1998) Nature 392 (6674), 353-358
3] Deckert,G., Warren,P.V., Gaasterland,T., Young,W.G., Lenox,A.L., Graham,D.E., Overbeek,R., Snead,M.A., Keller,M., Aujay,M., Huber,R., Feldman,R.A., Short,J.M., Olson,G.J. and Swanson,R.V.
Direct submission GenBank (1997)