ISAnsp5

  • Family IS21
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_011145 ND Anaeromyxobacter sp.
Anaeromyxobacter sp. K
Anaeromyxobacter dehalogenans 2CP-1
DNA section
IS Length : 2192 bp

Ends


IR Length : 39/50

IRL : TGTAGAGCGACAGGATAACTGACCCCCTTGCGACACAACTTCTGACCCCC
IRR : TGTCAAACGACAGGGGAAACTGACCCCCAAACGACAGAACTTCTGACCCC

Insertion site


Left flankDirect repeatRight flankDR Length
GCCGGAGCATACGCCGACGGTGGGGTGTGAAACAT5
GCGCACGCGGGGAAGCCTCGCGGCCACGAAGATCG5
CCCAGCGCGCTCTTCCGGGGCAACGTACGCAGTGG5
TCCCCCACCCCGCGTTCGTGCGGTACACCAGAGAT5
CACCCTTGCTCTTCTCGCGCTCGCCTCGGCCGGTC5

DNA sequence

TGTAGAGCGACAGGATAACTGACCCCCTTGCGACACAACTTCTGACCCCCTCTGCCCTCACCAAGGAGGCGGAGATGGGCAGCGAGATGGCGTTCATGGC
ACCCCCGACGACGGAGGTGCCGATGGTCGAGGCGGAGGCGGTCAGGCAGATGCGGGACCTCGCGGCGAAGGGCTGGGGCGCGAAGCGGATCGCGCGCGAG
CTCGGCGTGGCCCGCAACACGGTGCGTCGGTACCTGCGCGGCGCGGTGGCTGAGCTCCAGCGGCACCCTTCGCAGCAGCGGCTCGACGAGGTCCGGCGAG
CGGAAGCGGTCGCGCTGTTCGACGGCGAGGCTGAGGGCAACGCCGTGGTCGTAGCGCAGATGCTCGGCGAGCGCGGCATCGAGGCGAGCGTGAGGACCGT
GCAGCGCGCCGTGGCGGACCGCCGACGCGAACAGCGGGCAGCCGACGTCGCGACCGTCCGGTTCGAGACCGCGCCCGGCCGCCAGATGCAGATCGATTTC
GGCGAGCGTCAGGTGTGGATCGCCGGCGAGCGCGTGACGGTGCACTTCATGGCCGCAGTGCTGAGCTACTCGCGGCGGATCTTCGTGAAGGCGTTCCTGC
ACGAGCGTCAGGGCGAGTGGCTCGACGGGATCGCGTCGGCGTTTCAGCACTTCGGCGGCGTGCCGCTCGAGGTTCTCGGCGACAACACGCGCTGCCTCGT
CGCCGGCCGCAACCGAGAGGCGCAGACCGTGATCTTCCACCCGGCGTACGTCGCGTTCTGCCGCGACTGGGACGTGCAGCCGCGCGCCTGCCAGCCGTAC
CGCGCCCGAACCAAGGGCAAGACCGAGTCCGGCGTGAAGTACGTGAAGCGAAACGCGATCGCCGGTCGGCGGTTCGACTCGTTCGCACACCTGCAGGACC
ACCTCTCCGCGTGGCAGCTACTCGTGGATGGCCGCGTGCACGGGACGACGCACGAGGTCCCCGCGAAACGCTTCGACCGCGACGAGCGCGAGGCGCTGAG
GTCGCTGCCGGCGCGGCCGCTGCCGACGCACGGCCGTCGCCTGCAGCGGCGTGTCGCGAACGACTCGCTCATCGACATCGACACGGTCCGCTACAGCGTT
CCGCACCGCCTCGTGCGCGACCGCGTCGAGGCGCTCGTCACCGCCGACGAGGTTCGCGTCTTCCACGGCCGCGACGTCGTCGCGGTTCACGCGCGCTCGT
TCGAGCCGCACGCGCGGGTGATCGACCCCGCCCACCTCGACGGCCTCTGGCGCCGGCCTGCGGTAGAGAACGTCGTTCCCATCGCGCAGCCGCTCGCCGC
GCTCGGACGGACGCTCGACGACTACGCGGCCGTGATTGGCGGTGCCGCGTGAGCACCGAGATCACGCGGGCTCGCGTCCTCGAGAACCTCGGCCGGCTCC
GCCTCGGGCGCATCGGCGAACAGCTCGACGCGCTGCTCTCGACCGCGGCGCGCGGCGAGCCGACGTACCTCGACTTCCTCGACGCGATCCTGCGCGAGGA
GGTCGGGGCGAAGCAGCGGAAGCGCGTCGCGATGGGCATCCAGATCGCGCACTTCCCCGCGGTGAAGACCCTCGACGACTTCGACTTTAAGTTTCAGCCG
AGCGTCGACCAGAAGCTCGTCCGTGAGCTCGCCGTCAGCCGATACGTCGCGAACGCGGAGAACGTGCTCGTGTTCGGCCCGCCCGGCGTCGGCAAAACTC
ACCTGGCCATCGGGCTCGGACGCGCTGCGGTCGAGGCCGGCTACACCGTGCTCTTCACCAGCGCCACGGCGCTGCTGGGCGCGCTCTCGAAGGCGGAGAC
CGAGGGCCAGCTCGCGGAGCGGCTCGCGTTCTACGCGAAGCCGAAGCTGCTCGTCATCGACGAGCTCGGGTATTTGCCGTTCGAGAAGCGCAGCGCGCAC
CTGTTCTTCCAGCTCATCGCGAGGCGGTACGAGAAGGGCGCGATGATCATCACGACGAACCAGGTCGTCACGCAGTGGGGCACGGTCTTCGGCGACGAGG
TCCTCGCCGCGGCGATCCTCGACCGGCTCCTGCACCACAGCTTCACGCTGATGATCCAGGGCGAAAGCTACAGGCTGAAGCAGAAGCGGAAGGCGGGCCT
GCTCGGCAGGTCCGAGAAGGCGAACTGAACCCCGAGGCCGAGGGGGTCAGAAGTTCTGTCGTTTGGGGGTCAGTTTCCCCTGTCGTTTGACA
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
1278 bp425 aa751352+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MGSEMAFMAPPTTEVPMVEAEAVRQMRDLAAKGWGAKRIARELGVARNTVRRYLRGAVAELQRHPSQQRLDEVRRAEAVALFDGEAEGNAVVVAQMLGER
GIEASVRTVQRAVADRRREQRAADVATVRFETAPGRQMQIDFGERQVWIAGERVTVHFMAAVLSYSRRIFVKAFLHERQGEWLDGIASAFQHFGGVPLEV
LGDNTRCLVAGRNREAQTVIFHPAYVAFCRDWDVQPRACQPYRARTKGKTESGVKYVKRNAIAGRRFDSFAHLQDHLSAWQLLVDGRVHGTTHEVPAKRF
DRDEREALRSLPARPLPTHGRRLQRRVANDSLIDIDTVRYSVPHRLVRDRVEALVTADEVRVFHGRDVVAVHARSFEPHARVIDPAHLDGLWRRPAVENV
VPIAQPLAALGRTLDDYAAVIGGAA

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
780 bp259 aa13492128+No
ORF function : Accessory Gene
AG : IS21 helper

ORF sequence :

MSTEITRARVLENLGRLRLGRIGEQLDALLSTAARGEPTYLDFLDAILREEVGAKQRKRVAMGIQIAHFPAVKTLDDFDFKFQPSVDQKLVRELAVSRYV
ANAENVLVFGPPGVGKTHLAIGLGRAAVEAGYTVLFTSATALLGALSKAETEGQLAERLAFYAKPKLLVIDELGYLPFEKRSAHLFFQLIARRYEKGAMI
ITTNQVVTQWGTVFGDEVLAAAILDRLLHHSFTLMIQGESYRLKQKRKAGLLGRSEKAN

 

Blast result :
Comments
ISAnsp5 is 91% (istA, the transposase) and 94% (istB, the helper of transposition) aa similar to ISAnsp4.
References
1] ISfinder annotation (2009)
2] Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Saunders,E., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Kyrpides,N.,Ovchinnikiva,G. and Beliaev,A. (2008) Direct submission GenBank