ISAnsp4

  • Family IS21
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_011145 ND Anaeromyxobacter sp.
Anaeromyxobacter sp. K
DNA section
IS Length : 2195 bp

Ends


IR Length : 40/50

IRL : TGTAGAGCGACAGGATAACTGACCCCCTCGCGACACTAATTCTGACCCCC
IRR : TGTCAAACGACAGGAAGATCTGACCCCGTTACGACACTAAAACTGACCCC

Insertion site


Left flankDirect repeatRight flankDR Length
TTCCCCGCGTGCGCGGGGATCGACCCTCGGTCC5

DNA sequence

TGTAGAGCGACAGGATAACTGACCCCCTCGCGACACTAATTCTGACCCCCCCTCCCTCCTCGAACGCGGAGGCGGAGATGGGCAGAGAAGTGCTGGCTGT
GGCACCCCCGAGCGCGGAGGTGCCGATGGTCGAGGCGGAGGCGGTCCGGGAGATGCGCGCGCTCGCGGGACGAGGCTGGGGAGCCAAGCGCATCGCCCGC
GAGCTGGGTCTGGCGCGCAACACGGTGCGCCGCTACCTGCGCGGCGGGCCGGGAGCCGAGGTCCAGGAGCGGCCCGCGGCGCGCCGCCTCGACGAGACGG
CGCGGGCCGAGGCGGTGGCGCTGTTCGACGGCACCGCCGAGGGCAACGCGGTCGTGGTCGCGGACCTGCTCGCGGCGCGAGGCGTCGAGGCGAGCGTCCG
GACCGTGCAGCGCGCCGTGGCGGGGCGGCGACGCGAGCAGCGCGCGGCCGACCTCGCGACGGTGCGCTTCGAGACGGCGCCGGGCCGGCAGATGCAGATC
GAGTTCGGCGAGCGGAAGGTCTCGATCGCCGGCGAGCGAGTCACGGTGCACTTCCTCGCCGCGGTGCTCAGCTACTCGCGCCGGGGGTTCGTGAAGGCGT
TCCTCCACGAGCGCCAGGGGGAGTGGCTCGACGGCATCGCGTCCGCGTTCCGGCACTTCGGCGGCGTGCCCGCCGAGGTCCTCGGCGACAACACGCGCTG
CCTGGTCGCCGCGCGCAACCGCGACGCACAGACGGTGACGTTCCACCCGGCGTACCTCGCCTTCTGCCGCGACTGGGACGTGCAGCCGCGCGCGTGCCAG
CCGTACCGGGCGCGCACCAAGGGCAAGACCGAGTCCGGCGTGAAGTACGTGAAGCGCAGCGCCATCGCCGGCCGGCAGTGTGAGTCGTTCGCGCATCTCG
AGGCGCACCTCGCCGATTGGCAGCTTGCGGCCGACCAGCGCGTCCACGGCACGACCCACGAGCGCCCCGCAGAGCGGTTCGAGCGCGACGAGCGCCAGGC
GCTGCGACCGCTTCCGGCGAGGCCGCTGCCGACCCACGCGCGCCGCCTGCAGCGGCGCGTCGCGAACGACGCGCTCATCGACATCGACACTGTTCGCTAC
AGCGTGCCGCACCGGCTCGTTCGCGAGCACGTCGAGGCGCTCGTCACCGCGACCGAGGTCCGCGTCTACCACGGCCAGGACCTCGTCGCCGTCCACGCGA
GGTCCCAGGAGCCGCACGCTCGCGTCGTCGACCCCGCGCACTTCGAGGGGCTCTGGCGGCGCACGCCGGTCGAGAACGTCGTGCCGCTCGCGCAGCCGCT
CGCGGCCCTGGGCCGTAGCCTCGACGACTACGCGGCCGTGATCGGAGGTGCTGCGTGAGCACCGAAATCGTGCGCGCCCGCGTGCTGGAGCACCTCGGTC
GTCTGCGGCTCGGCCGCATCGCCGAGCAGCTCGATGCGGTCCTCTCCGGCGCCGCCCGCGGCGAGCCGACCTACCTGGACTTCCTCGACACGATCCTGCG
CGAGGAGGTCGGTGCCAAGCAGCGCAAGCGAGTGGCGATGGGGATCCAGATCGCGCACTTCCCGACCGTGAAGACGCTCGACGACTTCGACTTCAGGTTC
CAGCCGAGCGTCGACCAGAAGCTCGTCCGGGAGCTCGCTGTCGGCCGCTACGTCGCCCAGGCCGAGAACGTCCTCGTCTTCGGCCCGCCGGGCGTCGGCA
AGACTCACCTCGCGATCGCGCTCGGCCGCGCCGCCGTCGAGGCCGGCTACGCCGTCCTCTTCACCAGCGCGACGGCGCTTCTGGGCGCGCTCTCGAAGGC
AGAGACGGACGGCCAGCTCGGCGAGCGGCTCGCGTTCTACACGAAGCCGAAGCTCCTCATCGTGGACGAGCTCGGGTACCTGCCGTTCGAGAAGCGCAGC
GCGCACCTGTTCTTTCAGCTCGTCGCGCGGCGGTGCGAAAAGGGTTCGATGCTCGTGACGACGAACCAGGTCGTGACGCAGTGGGGAACCGTGTTCGGAG
ACGAGGTGCTCGCCGCCGCGATCCTCGACCGGCTCCTGCACCACAGCTTCACGCTGATGATCCAGGGCGAGAGCTACCGACTGAAGCAGAAGCGCAAGAC
GGGCCTGCTGGGTAGGGCCGAGAAGGCGAACTGACCGGGAGGGAGGGGGTCAGTTTTAGTGTCGTAACGGGGTCAGATCTTCCTGTCGTTTGACA
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
1233 bp410 aa1261358+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MVEAEAVREMRALAGRGWGAKRIARELGLARNTVRRYLRGGPGAEVQERPAARRLDETARAEAVALFDGTAEGNAVVVADLLAARGVEASVRTVQRAVAG
RRREQRAADLATVRFETAPGRQMQIEFGERKVSIAGERVTVHFLAAVLSYSRRGFVKAFLHERQGEWLDGIASAFRHFGGVPAEVLGDNTRCLVAARNRD
AQTVTFHPAYLAFCRDWDVQPRACQPYRARTKGKTESGVKYVKRSAIAGRQCESFAHLEAHLADWQLAADQRVHGTTHERPAERFERDERQALRPLPARP
LPTHARRLQRRVANDALIDIDTVRYSVPHRLVREHVEALVTATEVRVYHGQDLVAVHARSQEPHARVVDPAHFEGLWRRTPVENVVPLAQPLAALGRSLD
DYAAVIGGAA

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
780 bp259 aa13552134+No
ORF function : Accessory Gene
AG : IS21 helper

ORF sequence :

MSTEIVRARVLEHLGRLRLGRIAEQLDAVLSGAARGEPTYLDFLDTILREEVGAKQRKRVAMGIQIAHFPTVKTLDDFDFRFQPSVDQKLVRELAVGRYV
AQAENVLVFGPPGVGKTHLAIALGRAAVEAGYAVLFTSATALLGALSKAETDGQLGERLAFYTKPKLLIVDELGYLPFEKRSAHLFFQLVARRCEKGSML
VTTNQVVTQWGTVFGDEVLAAAILDRLLHHSFTLMIQGESYRLKQKRKTGLLGRAEKAN

 

Blast result :
Comments
ISAnsp4 is 58% (istA, the transposase) and 71% (istB, the helper of transposition) aa similar to ISSme2.
References
1] ISfinder annotation (2009)
2] Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Saunders,E., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Kyrpides,N.,Ovchinnikiva,G. and Beliaev,A. (2008) Direct submission GenBank