ISfinder Mirror Site

ISAcma34

Family IS630
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_009925	ND	Acaryochloris marina	Acaryochloris marina MBIC11017 plasmid pREB6 Acaryochloris marina MBIC11017

DNA section

IS Length : 1081 bp

Ends

IR Length : 20/26

IRL : AGCAGATGAACAAAATTAATTACATAGAATTTCCTGGTTGTAAACTACCT
IRR : AGCACCTAGCTAAAATTAATTACATAATCATCGCCGTAGGCTTTCAAAAC

Insertion site

Left flank	Direct repeat	Right flank	DR Length
AGAGACTAAA	TA	TCAGCCTGGC	2
TAGCAGCTTA	TA	ACTTTGGTTA	2
TGCATGAGTA	TA	TCGATAATGG	2
CTGATGTTTA	TA	AGACAGCCTA	2
TCAACGTTGA	TA	ATAGTTCAGG	2
GTGTGGAAGG	TA	TCAACTGGGG	2

DNA sequence

AGCAGATGAACAAAATTAATTACATAGAATTTCCTGGTTGTAAACTACCTGAGTCCTCTGTTTACATCCATTGATTTGATGTTATTAGTCCGTCCTATCT
CCACCGAAAGCTTACGCTTATTGCATCGGATTTATCACAGTAGTCGCCATCATCAGGTCAGACAACGCGCCCATTGCCTCATTTTGTTCGCCCAGGGTTG
GCCCCCCTACACCTTAGCCTCCCTTTTTAGTGTGAGTCCTAAAACGGTTTACAACTGGCTTAAGGCTTGGAACAACCGAGGATTTGCAGGACTCTATAAT
CACCCAGGTCGAGGTCGAAAACCGATGTTTAATCCCGACCAACAACAGCAGATTTATGAGTGGACTCAGGCATCTCCCATCCAACTCAATCAGGTGTTAG
CTCAGATTGAACAACAGTGGTCAGTACGCGTGTCAAAGGCCACAGTTAAACGCGTCTTGAAGCAGATGGACATGAGCTGGCATCGCTTTCGCCAAGGGAC
ATCAGGCCAGCCTTTATATGCCCACTACCTCGAAAAAAACAGCAGTTAGAACATCTCAAGGAACAGGAAGAGAAGGGAGACATCCATCTATTCTTTATGG
ATGAGAGTGGTTTTTCGTTAGTCCCCTGTATTCCCTATGGATGGCAACCCATAGGGACGTATCTGGAAATACCGACTCGTTCAAGTAAACGCTTGAATGT
TCTGGGGTTTTTGAGTCGACGACAAGGGCTACATGCTTATACATCAGAACAGACCATCACCAGTGAGGTTGTCAGTCATTGCATTGATACCTTTTTTGCT
GACGTGGAGTTGCCCACCGTCATTGTGGTGGATCAAGCCCCTATCCATACGAGCCAATCAATCTATGAGATGAAAGCAGAGTGGGCCGAACGGGGAATTA
CCTTGTTTGAGTTGCCAAGCTATTCTCCCCATCTAAATTTGATTGAGCGTCTGTGGCAATTCATGAAATATCAGTGGATTGAAATGAGTGCCTACTGGGG
GTGGTCATCTTTGGTCGAATATGTGGAAAGAGTTTTGAAAGCCTACGGCGATGATTATGTAATTAATTTTAGCTAGGTGCT

Recoding section

Recoding by frameshift
Frame
Type
Experimentally demonstrated

Stimulators :

Shine-Dalgarno sequence :
Secondary structure :

Recoding motif :

Protein section

ORF number : 3

ORF 1

Length		Begin	End	Strand	Fusion ORF
471 bp	156 aa	79	549	+	No

ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MLLVRPISTESLRLLHRIYHSSRHHQVRQRAHCLILFAQGWPPYTLASLFSVSPKTVYNWLKAWNNRGFAGLYNHPGRGRKPMFNPDQQQQIYEWTQASP
IQLNQVLAQIEQQWSVRVSKATVKRVLKQMDMSWHRFRQGTSGQPLYAHYLEKNSS

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
627 bp	208 aa	450	1076	+	No

ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

TRLEADGHELASLSPRDIRPAFICPLPRKKQQLEHLKEQEEKGDIHLFFMDESGFSLVPCIPYGWQPIGTYLEIPTRSSKRLNVLGFLSRRQGLHAYTSE
QTITSEVVSHCIDTFFADVELPTVIVVDQAPIHTSQSIYEMKAEWAERGITLFELPSYSPHLNLIERLWQFMKYQWIEMSAYWGWSSLVEYVERVLKAYG
DDYVINFS

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
998 bp	332 aa	79	1076	+	Yes

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MLLVRPISTESLRLLHRIYHSSRHHQVRQRAHCLILFAQGWPPYTLASLFSVSPKTVYNWLKAWNNRGFAGLYNHPGRGRKPMFNPDQQQQIYEWTQASP
IQLNQVLAQIEQQWSVRVSKATVKRVLKQMDMSWHRFRQGTSGQPLYAHYLEKKQQLEHLKEQEEKGDIHLFFMDESGFSLVPCIPYGWQPIGTYLEIPT
RSSKRLNVLGFLSRRQGLHAYTSEQTITSEVVSHCIDTFFADVELPTVIVVDQAPIHTSQSIYEMKAEWAERGITLFELPSYSPHLNLIERLWQFMKYQW
IEMSAYWGWSSLVEYVERVLKAYGDDYVINFS

Blast result :

Comments

ISAcma34 is 49% aa similar to ISTth6.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.

References

1] Swingley,W.D., Chen,M., Cheung,P.C., Conrad,A.L., Dejesa,L.C.,Hao,J., Honchak,B.M., Karbach,L.E., Kurdoglu,A., Lahiri,S.,Mastrian,S.D., Miyashita,H., Page,L., Ramakrishna,P., Satoh,S., Sattley,W.M., Shimada,Y., Taylor,H.L., Tomo,T., Tsuchiya,T., Wang,Z.T., Raymond,J., Mimuro,M., Blankenship,R.E. and Touchman,J.W. (2008) Proc. Natl. Acad. Sci. U.S.A.
2] ISfinder annotation (2009).