ISfinder Mirror Site

ISCth16

Family IS701
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_009012	ND	Clostridium thermocellum	Clostridium thermocellum ATCC 27405

DNA section

IS Length : 1373 bp

Ends

IR Length : 19/24

IRL : TTAGTGTTGTGCATTAAAAAGTAATAAATTGTATTACCAATAAAATAGCA
IRR : TTTGACTTGTGCAGTAAAATGTAAAACTTTATGCAGCGACCCCTAATTTG

Insertion site

Left flank	Direct repeat	Right flank	DR Length
TTTTTGGTTATT	ATAATTTTA	ATTGTGTCGGT	9

DNA sequence

TTAGTGTTGTGCATTAAAAAGTAATAAATTGTATTACCAATAAAATAGCAAAACAAAAGCATAAGCAGACCAAATGTGGTAGTTTTGAATTGATAGATAA
AAAACTATTTCCACGGGGGTCAAACTTATGCCTGATATAATAATAGAACAAAGCCAAGAAGTAATCAATTATATAAATATGCTAAAGTTGCCTATTTCTG
GAGCTTTGAAGAACCATATGGTTCATATGATTTCAGGCATAATAACCACCGAGGGAAACAAGAATATTTCTAATGTCTATTCAAGGCTTACCTGTAACCG
GAACCGGAGTAGTGGCTCAAGGTTCCTTGGAGAATATAAATGGAGTAACGAATACGTAGATTACAAGAGGATAAATCATTCTCTTAAAACTGTTCGTGAG
AATGTACCCGAGGGAACTGTAGGTTTTTTCATAGTAGATGACACTTTGAGTAAAAAAGACAATTCTACTAAGAAAATCGAAGGCCTCGACTATCATCATT
CTCACAGTGATGGTAAGACCATGTGGTCTCACTGTGTAGTTACTTCCCATTACAAAATCTCCGAGTACTCCTTACCACTTAACTTTAAGCTCTATCTTAG
AAAGCAGTTCTTTGGACAAAAAGCCAAGAAGCTTTTTAAGAATAAGCAAGAGCTGGCAATGCAGCTAATTGATGAATTTACGCCTGTTACAGAAACTACG
TATTTACTTGTAGATGCTTGGTATACATCAGGAAAATTGATGCTTCATGCTCTTAAGAGAGGGTATCATACTATTGGAAGAATAAAATCCAATCGTGTGA
TTTACCCAGGAGGCATTAAGACTAATATCAAAGAATTTGCCACCCATATATGTAGTAACGAAACCTGCATAGTGACAGCAGGAGACGATAACTATTACGT
ATACAGATATGAAGGTAAAATCAATGATCTTGAGAATGCCGTAATTCTTATATGTTGGAGCAAAAAAGCTCTTTCTGATACACCAGCATTTATCGTAAGT
ACCGATGTAAGCCTAACTACCTCCACTATTGTTGGATATTACCAGAACCGCTGGGATATTGAAGTGAGCTACCGATATCATAAGAACTCATTAGGGTTTG
ATGAATACCAGGTTGAATCATTAACTTCAATAAAGCGTTTCTGGAGTATGGTCTTTATGACCTATACTTTTCTTGAGCTCTTCAGGGTCTCTAAAAAGAG
AAGCTTGAAACTTGAGACCATTGGAGATACCATAGGGTATTTCCGCAAACAATATATGGTCTGTATTGCCAAGTTTGCATACTCTTGTGCAGAAAAAGGG
GTAAGTCTTGATGATGTAGTTGCCAAATTAGGGGTCGCTGCATAAAGTTTTACATTTTACTGCACAAGTCAAA

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
1218 bp	405 aa	128	1345	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MPDIIIEQSQEVINYINMLKLPISGALKNHMVHMISGIITTEGNKNISNVYSRLTCNRNRSSGSRFLGEYKWSNEYVDYKRINHSLKTVRENVPEGTVGF
FIVDDTLSKKDNSTKKIEGLDYHHSHSDGKTMWSHCVVTSHYKISEYSLPLNFKLYLRKQFFGQKAKKLFKNKQELAMQLIDEFTPVTETTYLLVDAWYT
SGKLMLHALKRGYHTIGRIKSNRVIYPGGIKTNIKEFATHICSNETCIVTAGDDNYYVYRYEGKINDLENAVILICWSKKALSDTPAFIVSTDVSLTTST
IVGYYQNRWDIEVSYRYHKNSLGFDEYQVESLTSIKRFWSMVFMTYTFLELFRVSKKRSLKLETIGDTIGYFRKQYMVCIAKFAYSCAEKGVSLDDVVAK
LGVAA

Blast result :

Comments

ISCth16 is 41% aa similar to ISNpu7.

References

1] ISfinder annotation (2009)
2] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina del Rio,T., Hammon,N., Israni,S., Dalin,E., Tice,H., Pitluck,S., Chertkov,O., Brettin,T., Bruce,D., Han,C., Tapia,R., Gilna,P., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Mikhailova,N., Wu,J.H.D., Newcomb,M. and Richardson,P. (2007) Direct submission GenBank.