ISfinder Mirror Site

ISCth15

Family IS21
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_009012	ND	Clostridium thermocellum	Clostridium thermocellum ATCC 27405

DNA section

IS Length : 2828 bp

Ends

IR Length : 26/42

IRL : TGTTTGGTGTAAAATAAAAATGCAGCAAGTTGCCACATAAAAGTACAACA
IRR : TGTTTTGTCAATAGGAAGTGCAACAAAATGACAAGAAAAATGCAACACCC

Insertion site

Left flank	Direct repeat	Right flank	DR Length
TATAATTAAAT	CAATATATT	AATTCGACTTTGT	9

DNA sequence

TGTTTGGTGTAAAATAAAAATGCAGCAAGTTGCCACATAAAAGTACAACAGTTGAGTTGGCAATAATTCAAATATTTTACAACAAAATCACATCTATGAA
CTGATCACTGTTATAGATTCCATCATCGATAACAGGTATAGGAAAATCACTTCTGATAATAGGATGGAAAAAACAAAATAGCCATTGCCAGCACCCCATA
AAAATAGTACCCTTTTAGTTGTCCAAATACTAAAGGGGGGTATTAGAAAGGATGCTGACTATGACTGATATAAAGTATATCAAAGATTTATTCGAAAAGA
AAGGCCTATCTCTTAGGGAAATTACAAGAGTAACCGGACATAATTTTAGAACAGTCCGGAAATATATTGATAAAGAGGATTGGTCACAACCTCTGGTTAA
TAGAACAAGGGAATCTTTGATTAATAAATATAAAGCAGATATTGATGAATGGCTGGAGAGTGACGTTGATGCACCAAGAAAACAGAGACATACGGCAAAA
AGAATTTTTAACAAACTGAAGCATAAATACAATAATGAATTTAACTTGTCCTACCGAACTGTTGCAAGGTATGTAAGCCTTAAAAAGAAAGCTTTGTATC
AAGACACTGATGGATATATACCTTTGGAACACCCTACTGGTGAGGCACAGGTTGATTTTGGCAGAGCTGCTTTTTTTGAAAACGGTATTAGGTATGAAGG
GTATTATGTTACCATGTCGTTTCCATACAGCAATGGAGGGTATATACAGCTTTTCAAAGGTGCTAATATAGAATGCCTATTACAAGGAATGAAAAAGATT
TTTGAACACATGGGAAAAGTACCGACATGTATCTGGTTTGACAATGATAAAACAATTGTCAAAAAAATATTTGCTAATGGAGAAAGAAAAGTTACTGAAG
CTTTTGCACGATTCCGCATGCATTACGGCTTTGAAAGTAATTTTTGTAATCCAAGCAGTGGGCACGAAAAAGGTCATGTTGAAAACAAGGTTGGATATTC
AAGAAGAAATATGCTAGTGCCCATACCGAAGTTTAAAGATATTGTAGAATTTAACCGTCAATTGCTTATCCAATGTGATGAAGATATGCAGAGGGAACAC
TACAAAAAGAATGTATTCATCAATATTTTATTTGAAGAGGACAAAAAAGCGATGCGGGATATTCCAAAAGCTGAATATGAAATATACCGCATCGAAAAGT
TAAAGTCGGATAAATATGGCAAACTGAACTTTGACAACAGAAAATACTCTTCCGGGCCTCAATATGCCCAGAGAGAATTAATGATAAAAGCAGATGCCTT
CTCGGTTGCAATTATGGATGAACAGTACAATACAGTTCAGGTACATAAACGTTTGTATGGAGAAGAGAAAGAGTCAATGAAGTGGGGGCCATATCTTGAG
CTAATGAGCCGTAGACCAACTGCTTTAAAGTATACCGGTTTCTTCCGGGAGTTGCCGCAAACTCTTCAGGATTATCTTACAGTATGTGACTATGAACAGA
AAAAAGGTGCTTTGCGACTATTAGTGAAGATGTTGGAACAAAGCGAACTTGATATAGCCATTGAAGCCTTTAGATTCTGTATCGAGAGGGGAATAAAAGA
TTTAGACAGCATATGGGCAAAATATTACACTATGGTCTGTACACACATACAAGTTCAAGATGTTTTACTCAACACTAAGACTCCTGATGTTGTGCCTTAC
ACTGTGGATAATAGCATATATGATAACCTGCTGGCTGGGGGTGTCCAATATGTATGAACTAATAGCCAAACATTGCAGAAGCCTGAAACTTGGTTCACGG
ATTGTTCAAAACTACAAAAATATTGAGGCCAAAACACATGAAGAATTTCTTGAGAAGCTATTGGCTATGGAAGTAGAAGCTCGTGAACGGAATCGTAGGA
ATCACATGCTTAAGCAGGCACGTTTTGATTTGATAAAGACCTTTGGAGACTATCGTTTTGAAAAAATGATAATCCCGAACAGTATTGATATAGAAGCATT
GAGAAATGCCTCGTTCATTGATAAAAAAGAAAATCTAATTCTCTACGGTCCTGTTGGACTAGGTAAAACGCATCTTGCTACTGCCATTGGTGTAGAAGCC
TGCAATCAGGGCAGACATGTACGATTTTTCAGGACAGCGTCCCTTGTCAATCAACTATTGGATGCAAAGGCAGAAGGAAGCCTAAAACGGTTCTTAAAGC
AGTTAGAGAAAGTAGAACTCTTGATTTGTGATGAATGGGGATTTATTCCCTTTGAACGTGAGGGCTCACAACTTTTATTCCAGGTCATTTCGGACTGTTA
CGAGCGTAAAAGTATGATTATAACTACAAATTTGGAATTCAGCAAATGGAACGGTATTTTTTACGATGAAAAGTTAACCAGTGCTATTATAGACCGTATA
ATCCACCATAGTCATCTAATCGTTTTTGACGGCCCTAGTGACCGACTGGTGAATTCACTTATGAGGAAACAGTGAGAAGAAAACGAATTGGGACTTATTA
AGTTGTATCACTTTTTAGCTGATGCTCTGAGGTTTAAAGTTTAAACAGGCAGCACAGCAGGTCTGCGTTATGCCATGCTACAATGTAAATTACAAGGGGC
GGAATCAAAAGTTGTATCACTCTTCCCTTCAGCCCCTCGGTAATTTCTAAAAAGCATGGCATATGAAATAAAGTCAAGAGAAAAAGCGAAGAGACTCTTG
ACTTTATTTCAAGCAGACCGGAAATACAGGGAAAACCTCAATTTTACATTCCATATTTTGTATAATACTGGTTAGATGGGGTGTTGCATTTTTCTTGTCA
TTTTGTTGCACTTCCTATTGACAAAACA

Protein section

ORF number : 2

ORF 1

Length		Begin	End	Strand	Fusion ORF
1506 bp	501 aa	252	1757	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MLTMTDIKYIKDLFEKKGLSLREITRVTGHNFRTVRKYIDKEDWSQPLVNRTRESLINKYKADIDEWLESDVDAPRKQRHTAKRIFNKLKHKYNNEFNLS
YRTVARYVSLKKKALYQDTDGYIPLEHPTGEAQVDFGRAAFFENGIRYEGYYVTMSFPYSNGGYIQLFKGANIECLLQGMKKIFEHMGKVPTCIWFDNDK
TIVKKIFANGERKVTEAFARFRMHYGFESNFCNPSSGHEKGHVENKVGYSRRNMLVPIPKFKDIVEFNRQLLIQCDEDMQREHYKKNVFINILFEEDKKA
MRDIPKAEYEIYRIEKLKSDKYGKLNFDNRKYSSGPQYAQRELMIKADAFSVAIMDEQYNTVQVHKRLYGEEKESMKWGPYLELMSRRPTALKYTGFFRE
LPQTLQDYLTVCDYEQKKGALRLLVKMLEQSELDIAIEAFRFCIERGIKDLDSIWAKYYTMVCTHIQVQDVLLNTKTPDVVPYTVDNSIYDNLLAGGVQY
V

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
1 bp	241 aa	0	0	+	No

ORF function : Accessory Gene
AG : IS21 helper

ORF sequence :

MYELIAKHCRSLKLGSRIVQNYKNIEAKTHEEFLEKLLAMEVEARERNRRNHMLKQARFDLIKTFGDYRFEKMIIPNSIDIEALRNASFIDKKENLILYG
PVGLGKTHLATAIGVEACNQGRHVRFFRTASLVNQLLDAKAEGSLKRFLKQLEKVELLICDEWGFIPFEREGSQLLFQVISDCYERKSMIITTNLEFSKW
NGIFYDEKLTSAIIDRIIHHSHLIVFDGPSDRLVNSLMRKQ

Blast result :

Comments

ISCth15 is 51% (ORFA, the transposase) aa similar to ISBad1 and 63% (istB, the helper of transposition) to ISBlo2.
There is only one copy in the genome.

References

1] ISfinder annotation (2009)
2] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina del Rio,T., Hammon,N., Israni,S., Dalin,E., Tice,H., Pitluck,S., Chertkov,O., Brettin,T., Bruce,D., Han,C., Tapia,R., Gilna,P., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Mikhailova,N., Wu,J.H.D., Newcomb,M. and Richardson,P. (2007) Direct submission GenBank.