ISfinder Mirror Site

ISCth12

Family IS21
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_009012	ND	Clostridium thermocellum	Clostridium thermocellum ATCC 27405

DNA section

IS Length : 2497 bp

Ends

IR Length : 25/38

IRL : TATTTATTGCAAAATAGAAACGGACAAAGTTGCAAAAAAAATTCCCCAGT
IRR : TGTTTTTTGCAAGTGAGATCTCCCCACTTTTGCAAAATTTTCCCCAGGCA

Insertion site

Left flank	Direct repeat	Right flank	DR Length
CTTTCATTTAAAA	GAAATATG	TGGTAATATGTA	8
ATTATTTTTCTGC	TTTCAA	TAATACTTTTTAAT	6

DNA sequence

TATTTATTGCAAAATAGAAACGGACAAAGTTGCAAAAAAAATTCCCCAGTTTTGCAACTATGAATTCCCCACTATTGCAAAAGGCCATTGAAGGCACCAC
ACCAAAATAATATCCTTGTGATTGATCGACCAAATCAGTTACAAGGAGGATTGGAGGATGCTATCAATGACCCAAATCAAGGATATCAGAAAAATGTATT
TTGAGGAAGGGAAAAATATCAGCCAGATAGCCAGAGAAACCGGCCATGATCGCAAAACGGTGAGAGCATATCTTGACAAAGTGGACTGGAACCAGAAGCC
ACCGAAAGTGAAGAAGGAAACAGCCTTTCCGAAACTTAATCCATACAAGGATGACATTGACACATGGCTAAACGAGGATAAAAAGGCCAGGCGCAAGCAA
AGACATACAGCAAAACGAATATACAACCGGCTGGTGGAAAAGTACGGAGAACGCTTCAACTGTTCCTACAGGACCGTAGCAGGATATGTAGCTGTGAAGA
AAAAAGAGATATTCAACGCAAGGGAAGGATTCCTGCCTTTAGAGCACGTACCAGGTGAAGCCCAGGCAGACTTTGGCGATGCTGACTTTTATGAAAATGG
CAGGCACTACAGGGGTAAAAGTCTGACTTTATCATTTCCCCACAGCAACAAAGGATATACCCAGCTATTCAAGGGAGAGAACCAGGAATGCCTGTTCGAG
GGCTTGAAGGCGATATTTGAGCACATAGGTGGAGTGCCGCCAAGGATATGGTTTGATAATGCCAGCACCATAGTAGCTAAGGTAATAAAGGGCGGAGGCA
GGAACCTGACAGATGATTTCATGCGTTTCATGGAGCATTACCGTTTCAAAGCAGTATTCTGCAATGTAGATGCCGGGCATGAAAAAGGCAATGTGGAGAA
CAAGGTCGGCTATCACAGGAGAAACATGCTGGTGCCGGTACCACGTTTTGAAGACATTAGTGAATTCAACAAAGAACTCCTGATTAGGTGTGAAGAAGAT
GCCAAAAGGCAGCATTACCGAAAGAACGGTACGATCGAAGAACTATACAGGGATGATAAGGCAGCCCTGCTGGAGCTGCCCAAGACAACTTTTGATACAA
GCAAATACATAACAGTGAAGACAAACGGATATGGCAAATTTCTGCTCAACAAAGGCCTGCACGAATATTCCTCAGCGCCAAAATTCGCAAACAAATATGT
ACTGGTCAGGCTGACTGCCTTTCATGTAACAGTGCTTGACGAAAGCCATCGGGAGATAGTGCGTCATGAGAGACTCTACGGCGACTACAAGCAGCAAAGC
ATGCAATGGCTGCCATATCTGACTCAGCTGGCACGGCGACCGGGGGCATTGAAATACACAGGTATATATCAGATGCTGCCACAGCCTGTGAAAGAATACA
TGGAAGAGCTAAGCAAGCAAGACAGAGGGAAAGTATTAAGAGTAATTGCTGATCTGACACAGAAGAGCAGCTTCGAAAAGGCCATTAAGACTGTCAGTAC
TGCCCTGTCCTATGGTGCTGCCGATGTGGACAGCCTGATAAATCTGCACAGATATTTGTATGAAAAAGTGCTGCAGCTGGAGCCGATACATTTGCCCGAG
CATATACCTCACTTAAACAGATATGTGCCTGATTTTATGGCATATGACAGAAGTCTCAAGGCAGGTGAAGAAAAATGCTGACATCTGATATTGCAGCATG
TTGCAGAAGGCTTCGCCTCAGCCGGAACATAGTGGAGATGTCAGGTAAAATACAGGCAGTCAGCCACCAGGAATACCTGCTTAAACTTCTTCAATCAGAG
ATCCGGCATCGTGAAGAACTGAAGAAAGACAAACTGCTGAAAAAAGCAGGTTTCTATACCATAAAGACATTTGAAAGCTTCCGGTTTGATGAAGTAAAGC
TGCCCAGTGGCGTTACTCCGGAATATCTTAAAGAGTGCGAGTTTATCGAAAACAAACACAATATCGTCATGTACGGCAATGTGGGCACAGGAAAGACGCA
TCTTTCAATTGCTTTAGGTGTAGAGGCCTGCAAGAAGGGATTGGAGGTGAGATTTTTCAGGACATCAGCGCTCGTGAACAGGCTGGCGGAACAAAAGAAA
GCCGGAACATTGTCAGGCTTCTTGAAGGACCTGAATAAAGCAGATCTTTTGATCTGTGACGAATGGGGCTACGTCCCCCTCGACCGTATAGGAGCGCAGT
TATTGTTTGAGGTCATATCCGAGTGTTATGAACGCAAATCGGTGATCATCAATACCAACATAGAATTTTCAAGGTGGGTGAACGTGTTCTATGACGAGCA
GATGACAGGCGCCATTATCGACCGGCTACTTCATCACTGTCACTTGCTACTGTTTCCCGGCCAGAGCAACAGGATGCGCGAAGCTGTACTAAACACATAA
AAAACTTACGGGAAAGTCACTGGGTTTACATAAAAATTCCCCACCGGTGCCTGGGGAAAATTTTGCAAAAGTGGGGAGATCTCACTTGCAAAAAACA

Protein section

ORF number : 2

ORF 1

Length		Begin	End	Strand	Fusion ORF
1515 bp	504 aa	167	1681	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MTQIKDIRKMYFEEGKNISQIARETGHDRKTVRAYLDKVDWNQKPPKVKKETAFPKLNPYKDDIDTWLNEDKKARRKQRHTAKRIYNRLVEKYGERFNCS
YRTVAGYVAVKKKEIFNAREGFLPLEHVPGEAQADFGDADFYENGRHYRGKSLTLSFPHSNKGYTQLFKGENQECLFEGLKAIFEHIGGVPPRIWFDNAS
TIVAKVIKGGGRNLTDDFMRFMEHYRFKAVFCNVDAGHEKGNVENKVGYHRRNMLVPVPRFEDISEFNKELLIRCEEDAKRQHYRKNGTIEELYRDDKAA
LLELPKTTFDTSKYITVKTNGYGKFLLNKGLHEYSSAPKFANKYVLVRLTAFHVTVLDESHREIVRHERLYGDYKQQSMQWLPYLTQLARRPGALKYTGI
YQMLPQPVKEYMEELSKQDRGKVLRVIADLTQKSSFEKAIKTVSTALSYGAADVDSLINLHRYLYEKVLQLEPIHLPEHIPHLNRYVPDFMAYDRSLKAG
EEKC

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
726 bp	241 aa	1675	2400	+	No

ORF function : Accessory Gene
AG : IS21 helper

ORF sequence :

MLTSDIAACCRRLRLSRNIVEMSGKIQAVSHQEYLLKLLQSEIRHREELKKDKLLKKAGFYTIKTFESFRFDEVKLPSGVTPEYLKECEFIENKHNIVMY
GNVGTGKTHLSIALGVEACKKGLEVRFFRTSALVNRLAEQKKAGTLSGFLKDLNKADLLICDEWGYVPLDRIGAQLLFEVISECYERKSVIINTNIEFSR
WVNVFYDEQMTGAIIDRLLHHCHLLLFPGQSNRMREAVLNT

Blast result :

Comments

ISCth12 is 55% (ORFA, the transposase) and 67% (istB) aa similar to ISBlo2.

References

1] ISfinder annotation (2009)
2] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina del Rio,T., Hammon,N., Israni,S., Dalin,E., Tice,H., Pitluck,S., Chertkov,O., Brettin,T., Bruce,D., Han,C., Tapia,R., Gilna,P., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Mikhailova,N., Wu,J.H.D., Newcomb,M. and Richardson,P. (2007) Direct submission GenBank.