ISfinder Mirror Site

ISCth2

Family IS30
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_009012	ND	Clostridium thermocellum	Clostridium thermocellum ATCC 27405

DNA section

IS Length : 1224 bp

Ends

IR Length : 18/25

IRL : GGGAAGTTGTAAAATAAAATGCCGTAATATATAGAAAACTCATGGTAATC
IRR : TATAAATTGCAATATTAAATGCCGTGCAAAATTTGGTAAATCCATTGCTT

Insertion site

Left flank	Direct repeat	Right flank	DR Length
AATGTTTTTTCTTTAA	TGGTTTCTTTTATT	CGATGTTTGTGT	14
AATTGGAAAAACAAAA	AAACAAATAAAAT	AGAAAATATTTCT	13
CTCGGCGGAAGTGGG	ACATGAGGAAGTGAA	AATAAAAGAAGTT	15
AAAAAATGGTTATT	ACTTTATATTATTTT	GAAGATTTGACATTGA	15
TCAAGGTTTCCAATC	TGTATGGATTTATAT	GGATTTATTTATTTT	15
CAAATACCATTTCTCTCTCTAATAAATCAC		ATTTTTTTGCCACATATACAGAGAATTTGA	0
TAATAAACAAGATAT	ACAAAAATTTAAAGA	AGAAATTTCGGTTTC	15
TTGTAAACAGTATAA	ACGTTGCATTTCTTG	ATAAATAATTCTTGC	15
ATTGGACACCTAAATCATACATGATTTCTC		TTTATGGACAGACATATAAAAAAATCTTGA	0
TTACTGTTGCATTTAA	TTTTACAACGAACC	AAACAATTTTCTTTAC	14

DNA sequence

GGGAAGTTGTAAAATAAAATGCCGTAATATATAGAAAACTCATGGTAATCCATTGTAAAATGTTTAATAACCAAAGAAAAACATTAGCAAAGGAGTACCA
TGAGTTATATATGAATAATAACACAGAAACAAGAAAAAACAAACACCTAAATGAAAGAGAAAGATACGCCATAGAGTTGTACCTAAAAGAAAAGTATACA
GTAACGGAAATAGCAAAGAGGTTGGGCAGGCACAGAAGGACAATAGAAAGAGAAATAACCCGTGGGACAATATATTTACAAAATAGTGATTTAACATACA
GAAAAGAGTATTGTGCAGATGTAGCCCAAAGGAGATATGTAGAGAACGGGAAGAATAAAGGTCCACGGTTAAAGATAGGAAATGACCATGAATTAGTGAG
GTATATAGAATCAAAGATAATAAATGAAAAATATTCTCCTGATGCTGTTATTGGACAAATAAAAGCAAAGGGACTGAAGTTTAAAACGAGTATCTGCACG
AAAACACTATATAACTACATTGATAGAGGGGATGTATTTTTAAGACTAACGAATAAATATTTGCCAGTGAAAAAGGATGGTAAAAAACGCATATATCAAA
GGGTAAAGAAGATAGCACTGAAAAATCTTAAGGGAAGCAGCATAGAGGAAAGGCCGAAAGAAGTTAACGATAGGAAGGAATATGGACACTGGGAGATGGA
CTGTGTAGTAGGCAGAAAAAATAGCGCAGCAGTATTACTGGTATTAAGTGAACGAAAGACAAGAGAAGAAATAATTCTTAAACTACCAGACAAAACGCAG
GAATCAGTAATCAAAGCAATAGATGAATTAGAAAGGAAGTATAGAAGGAAATTTAGGGAGAAGTTTAAAACGATAACAGTAGATAACGGGACAGAGTTTT
TGGACTATAGAGGGATAGAGAAATCAAAAACAGAGCCAGGCAAGGACAGGACGAAAGTGTATTATGCTCATCCTTATAGTTCTTGGGAAAGAGGAACGAA
CGAAAATATTAATAAACTGATAAGAAGATTTATACCAAAAGGAACAGATATATCAAAAGTAAGTAAAGCAAAAATAAAAAGTATAGAGAGATGGATTAAT
GAATATCCAAGAAGAATGTTTGGTTATAGGTCAGCCATAGAAATGGCGGTATGATGTATAGAATTTATTCCAGTAAGCAATGGATTTACCAAATTTTGCA
CGGCATTTAATATTGCAATTTATA

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
1113 bp	369 aa	42	1154	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MVIHCKMFNNQRKTLAKEYHELYMNNNTETRKNKHLNERERYAIELYLKEKYTVTEIAKRLGRHRRTIEREITRGTIYLQNSDLTYRKEYCADVAQRRYV
ENGKNKGPRLKIGNDHELVRYIESKIINEKYSPDAVIGQIKAKGLKFKTSICTKTLYNYIDRGDVFLRLTNKYLPVKKDGKKRIYQRVKKIALKNLKGSS
IEERPKEVNDRKEYGHWEMDCVVGRKNSAAVLLVLSERKTREEIILKLPDKTQESVIKAIDELERKYRRKFREKFKTITVDNGTEFLDYRGIEKSKTEPG
KDRTKVYYAHPYSSWERGTNENINKLIRRFIPKGTDISKVSKAKIKSIERWINEYPRRMFGYRSAIEMA

Blast result :

Comments

ISCth2 is 69% aa similar to ISTde2. There are 10 copies in the genome.

References

1] Miriam Land (2009) Direct submission
2] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina del Rio,T., Hammon,N., Israni,S., Dalin,E., Tice,H., Pitluck,S., Chertkov,O., Brettin,T., Bruce,D., Han,C., Tapia,R., Gilna,P., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Mikhailova,N., Wu,J.H.D., Newcomb,M. and Richardson,P. (2007) Direct submission GenBank.