ISfinder Mirror Site

ISAcma26

Family IS21
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_009925	ND	Acaryochloris marina	Acaryochloris marina MBIC11017 Acaryochloris marina MBIC11017 plasmid pREB1 Acaryochloris marina MBIC11017 plasmid pREB2 Acaryochloris marina MBIC11017 plasmid pREB3 Acaryochloris marina MBIC11017 plasmid pREB4 Acaryochloris marina MBIC11017 plasmid pREB5 Acaryochloris marina MBIC11017 plasmid pREB6 Acaryochloris marina MBIC11017 plasmid pREB7 Acaryochloris marina MBIC11017 plasmid pREB8

DNA section

IS Length : 2669 bp

Ends

IR Length : 11/15

IRL : TATTAGACGACAATCGAGCTGACGGATTAATGACGATGTCACGTCGGCTA
IRR : TATTGGATGTCAACCCCATAGGCGATGTCGCGACAACCACAAGGCGGATC

Insertion site

Left flank	Direct repeat	Right flank	DR Length
ACAGACATAC	CGAGGG	CTTTCGACAG	6
AAGCGATGCC	AGCTTA	TGTCCATTTG	6
TATTAAATCC	TTCCAC	AAAACCACTG	6
GATGTAGAGC	ATGAGG	TTCGTCCCTG	6
CGGCAATTCC	TCAGAT	AGTTGAACCA	6
GTCGATATCG	CCCAAT	ACTGCTACCA	6
CGGACCAACC	ATTGCC	ACGGTCTAGA	6
ATTGATGAAT	ACCATC	ATCAGTCGAA	6
TGTTTCATAA	ATCTTC	TGTGTCACAG	6
GTTTCTTGAG		GCTAAATTAT	0
AACGCTCCTC		ATTCAGAGGA	0
GTTCAAAGCT		GCCCAAATTT	0

DNA sequence

TATTAGACGACAATCGAGCTGACGGATTAATGACGATGTCACGTCGGCTATAGCTATGAAGAAACCAGGCATCCCCTGAGAAGGATAAAACTAGATCTCA
ATTCCCTAGAAATGATGTGTGAGATCGCGTTTCTAGAATTCAATCTCAAGAAAAAAGACACTTTTTACATCCCTAATCAATAGAGGGCTGTACAAGTGCC
TAACAAAAGAATTGATTCCTATCATACTCGTGTTTATATGAACGGAAGAGACCTCGGCTTAAAACAAGCTGATGCAGCTTACATTGCCGAAATATCTACA
AGAACCGGACAACGAATTGAGGCCGGCACCCATCAACCTAATCGCGGCCGCCTTCAGGACCAACGCACCGTTCCCGACCCCTTAGCTGATGTGTGGGAAG
ACGAGCTAGAACCAATGCTGCGTCGAGACCCACGCCTCAAACCCATGACCCTGTATGAGTACCTGCAGGATAAGTATCCAGGCCAGTATCCCCAAGTCCT
GCGGACCCTACAACGTCGGGTGAGAACGTGGAAAGCCTTACATGGACCAAGCCCTGAAGTGATGTTTGAATTGCGTCATGAACCAGGGGTACAAGGGTTC
TCCGATTTTACAGAACTCAAAGGCATCACGATTACCATTGCCGGCAAACCCTTTGAGCACCTGATTTACCATTACCGTCTGGGATACAGCGGCTGGCGAT
ATGCCCAGATCATCGAAGGAGGCGAAAGCTTTGTCGCCCTCTCAGAAGGATTGCAAAATGCCTTTGCAGCCTGTGGAGGTGTTCCTACACAGCATCGTAC
TGATAGTTTGAGTGCAGCCTATCGCAACATGGGCGGCCGCCGGTCCAAAAACCTCACTCGTCTGTACGACGAACTGTGTGACCACTATCGGCTAGAACCC
ACTCGTAACAACAAAGGTGTAGCCCATGAGAATGGCTCCATTGAATCTCCCCATGGTCATCTGAAGAACCGAATTAAGCAGGCGATCTATCTGCGCGGCA
GTGCAGATTTTACGAGCGTTGCTGAGTATCAAGCCTTAATTGATGCACAGGTTGCCAAGTTGAATCAGCAGTGCCAAACCAAGTATGAGCAAGAGAAAGA
CCATCTACAACCACTGCCCAAATATCGAACCCCTGACTATGAAGTGCTCACGGCTAAAGTCAGCAAACGCAGCACCATCGATGTTCGCTGCATTCTATAC
ACCGTCCCTTCTCGACTGATTGGTCGTCAATTGGAACTGCATCTATACCATGACCGGATTGTCGGCTATCTGGAGCGACACCCGGTGGTGGAATTGCCGA
GGAAGCGCGTCAGTGGCAAAGGCAAACGTCGCGACCGTTGCATCAACTATCGCCATGTTATTGGTTCAATGCGATTGAAGCCTCGTGCTTTTATCTATTG
CACCTGGCAATCAGACCTACTTCCCAATCCTGAATACCGCCAAATCTGGGAACAGCTCAAAGCCCAATTTGACCTGGAGCAGGCTGCCAAGATCATCGTG
GAAGCCCTGTATATTGCTGCGGTTCAAGATAAAGAACAGGCCGTAGCAGTGTACTTACAGCAGCAGCTTCGCTCATCCAGCCTTACCCTCAATCGCCTGA
AAAAACAGTTTGAGCCGCCTCAGATGAAGCAGGTTCCTGAACTCAGCATTGAACAACATTCACTTGAACTTTATGACAAACTCCTCCCCTCCTGCTCAGT
CCCCGCTGAGCCCCTACCAGCACCTGAGCCTCTATTTAAAAAAGCTCAGGCTCTCCCACATGTTGACCCATTGGGAATCTATCGAATCCCAAGCCATGCA
GGAAAACTGGTCTTATGCGGAATTCTTACTAGCCTTGTGCGAAACGGAGGCCCAACGAAGAGAACAAGCTCGTCTAAAACGTGCCCTCACCGAAGCCAGG
CTCCCAAACGCAAAAAGTTTTACCAACTTTGACTTTAGCCATTGTCCCCAGCTCAATCCAGCTCCCTTGATGCAATTAGCCGCAGATCCGGGTTGGTTGG
AGCGCGCCGAGAATTGCCTTATTCTGGGGCCCTCGGGTGTTGGAAAAACACATCTGGCCACTGGGGTGTCCAAAAAGATGCTGGAATTCGGTAAGCGGGT
GAAGTTCTTTGCAGCCAACGCATTGGTCCAGCAACTGCAACAGGCGAAGCTCCAACTGCAGCTGCATCCAATGCTCAAAAAACTGGACCGCTATGATCTG
TTGATCTTGGATGACTTGGGCTATTGCAAAAAGTCGGAAGCGGAAACGTCCGTTCTGTTTGAATTAATTGCGCATCGCTATGAACGAAAAAGTTTGTTGA
TTACAGCGAATCAACCCTTCAGCCAATGGGATGACATTTTCACTGATTCGATGATGGCGGTGGCTGCCATTGATCGCTTAATTCATCATGGCTTGATTAT
CGAAATTCAAGCCGATAGTTATCGGCGCAAATCAGCGACTCAGAGAACGGCTCAAACTCAGTCTCAACCTCAAAAATCCCAGTCCAAATAATCGAGCGAT
CACTGGTTGTCATTTCGATCACGGGTTGTCGTTTTGTTCTGAAGGTATCGTATGAGGTCTGAAAAAGAGCTAACGCAAAGGGAGTGTACGCCAAATAATA
CGGTTCAAATTTGGGGGTTGATCCGCCTTGTGGTTGTCGCGACATCGCCTATGGGGTTGACATCCAATA

Protein section

ORF number : 2

ORF 1

Length		Begin	End	Strand	Fusion ORF
1737 bp	578 aa	196	1932	+	No

ORF function : Transposase
Description :

ORF sequence :

VPNKRIDSYHTRVYMNGRDLGLKQADAAYIAEISTRTGQRIEAGTHQPNRGRLQDQRTVPDPLADVWEDELEPMLRRDPRLKPMTLYEYLQDKYPGQYPQ
VLRTLQRRVRTWKALHGPSPEVMFELRHEPGVQGFSDFTELKGITITIAGKPFEHLIYHYRLGYSGWRYAQIIEGGESFVALSEGLQNAFAACGGVPTQH
RTDSLSAAYRNMGGRRSKNLTRLYDELCDHYRLEPTRNNKGVAHENGSIESPHGHLKNRIKQAIYLRGSADFTSVAEYQALIDAQVAKLNQQCQTKYEQE
KDHLQPLPKYRTPDYEVLTAKVSKRSTIDVRCILYTVPSRLIGRQLELHLYHDRIVGYLERHPVVELPRKRVSGKGKRRDRCINYRHVIGSMRLKPRAFI
YCTWQSDLLPNPEYRQIWEQLKAQFDLEQAAKIIVEALYIAAVQDKEQAVAVYLQQQLRSSSLTLNRLKKQFEPPQMKQVPELSIEQHSLELYDKLLPSC
SVPAEPLPAPEPLFKKAQALPHVDPLGIYRIPSHAGKLVLCGILTSLVRNGGPTKRTSSSKTCPHRSQAPKRKKFYQL

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
819 bp	272 aa	1673	2491	+	No

ORF function : Transposase
Description :

ORF sequence :

MTNSSPPAQSPLSPYQHLSLYLKKLRLSHMLTHWESIESQAMQENWSYAEFLLALCETEAQRREQARLKRALTEARLPNAKSFTNFDFSHCPQLNPAPLM
QLAADPGWLERAENCLILGPSGVGKTHLATGVSKKMLEFGKRVKFFAANALVQQLQQAKLQLQLHPMLKKLDRYDLLILDDLGYCKKSEAETSVLFELIA
HRYERKSLLITANQPFSQWDDIFTDSMMAVAAIDRLIHHGLIIEIQADSYRRKSATQRTAQTQSQPQKSQSK

Blast result :

Comments

ISAcma26 is 64% aa similar to ISEc10.
This IS has atypic IRs: it begins with 'TA' instead of 'TG'.

References

1] Swingley,W.D., Chen,M., Cheung,P.C., Conrad,A.L., Dejesa,L.C.,Hao,J., Honchak,B.M., Karbach,L.E., Kurdoglu,A., Lahiri,S.,Mastrian,S.D., Miyashita,H., Page,L., Ramakrishna,P., Satoh,S., Sattley,W.M., Shimada,Y., Taylor,H.L., Tomo,T., Tsuchiya,T., Wang,Z.T., Raymond,J., Mimuro,M., Blankenship,R.E. and Touchman,J.W. (2008) Proc. Natl. Acad. Sci. U.S.A.
2] ISfinder annotation (2009)