ISAau1
- Family IS3
- Group IS407
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_008711 | ND | Arthrobacter aurescens | Arthrobacter aurescens TC1 Arthrobacter aurescens TC1 plasmid pTC2 Arthrobacter aurescens TC1 plasmid pTC1 |
DNA section
IS Length : 1257 bp
Ends
IR Length : 20/25
IRL : TGGTTGGGTCCCCGTGTAGGAGTCCGGTGACTATGTGAGTTTCGTGTCCG
IRR : TGATTGGGCCCCCTTTAGCAGTCCAGCGGCTATGCGATAGCTGATTGGTG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CACCGTTGCTGCAAAC | CAAG | CTCCCGGCCATTTCAAC | 4 |
| CGGTCTGTCTTTGGCG | GCGG | CCCTGGCGGGTAAGTG | 4 |
| GGAACGCATGAGGATC | CGCA | GGCTGAGGCCTTCTGAC | 4 |
| AAGTCCACGCCGCGTT | TGAT | CGTGCCGGGAACGAGG | 4 |
| CAGTCATGGTCCAACC | GACC | CTACGCCGCCAAGGCG | 4 |
| GCAGAGACCACGTGCC | TTCG | GGCCGTGGCCTCTGTT | 4 |
| ACGCAACCGAGGAGTA | ATTG | GTCTGCGCGCGTGTCC | 4 |
| CAGTACGACGGCGATC | ATCA | CCTTGAGCAGGAACAG | 4 |
| GCTCAGCAACGGAGCG | TAGT | AAGTCCATCTGACTGG | 4 |
| AAAGCTTTTGGGGATG | ATAT | GACTTCGCTAACGACT | 4 |
| GGCACAGACATGTGCG | GCTC | CCCAACCCCACGGACC | 4 |
DNA sequence
TGGTTGGGTCCCCGTGTAGGAGTCCGGTGACTATGTGAGTTTCGTGTCCGAGAATGGACGCAGGAGGAAATTCACTAGTTATGGCACGTAGGCATACCCC
CGAGCAGGTCATCGCGAAGGTCCGGCAGGGCCAGAAAATGCTCAATGAAGGACGGCCCCTCATCGAGGTCGTCAAGGAACTGCAGGTCACCGAGGCGACC
TGGTACCGGTGGCTGAACCAGTACGGGTCCGAGAAGAACGCCGAGGCGACGAAACGGACCAAGGAGCTCGAGAAGGAGAACGCGAGGCTCAAGCGGCTGC
TGGCCGAGAAAGAGCTAGCCATCGACATCCTGAACGAGGTGGCCAAGGGAAAATTCTGAGCCCCGAACCACGCCGCCGTGCCGTGCGCATGGCGATGGAG
AAGTTCGGGGCGTCGGAACGCTTTGCCTGCAAAGTGCTGGGGCAGAACCGCTCCGCCCTGCGCAAGAAGAAACCCGAAATGAGTTTCGAGGAAACACGGC
TGCGCGCTGACCTGCGGGCAGTCGCGCAGAAACACCCGGCGTGGGGCTGGAAGAAGGCCCGCTGGCACCTGCGTGCCCAGCCGCAGTGGCAGGACGTGGC
GCTGAACAAGAAGCGGGTACGCCGGCTCTGGCGCGACGAGGGACTGGTTTGTAAACCCAAGCCGAAGAAGAAGCGCCGGACCGGCCCGGACGCCGGGGAA
CAGAAGCGCCTGAAAGCCGAATACCCGATGCACGTGATCAGCTTCGACTTCCAGTCCGACGTCACCTCCTGCGGGCGCCACATCCGCTTCTTCAACGTCA
TCGACGAATGCACCCGCACGGCGCTGGCGATCGTGCCGCGGCGCTCGTTCAAGGCTTCCGACGTGGTCGCCGTGCTGGAGAACATCATCGCCGAAACCGG
TATCGAACCGGCGTACGTGCGCTGCGACAACGGACCGGAATTCACCGCCGCCGCACTGATCGAATGGTGCAGCACAGCCGGTGTGAAGACCGCGTTCATC
GACCCGGGATCGCCCTGGCAGAACGGGTTCATCGAATCATTCAACGCCCAATTCAGAAGGGAACAACTCTCCGGAGAAATCATCGACACCATGGCCGAAG
CAAAGTACTTGGCCGACGAATGGAAAGACATCTACAATCATGAACGGCCCCACGGATCCCTGGATGGAATGACACCATCCAACTACTGGAATCAGTGGAC
CGCAGACCACCAATCAGCTATCGCATAGCCGCTGGACTGCTAAAGGGGGCCCAATCA
CGAGCAGGTCATCGCGAAGGTCCGGCAGGGCCAGAAAATGCTCAATGAAGGACGGCCCCTCATCGAGGTCGTCAAGGAACTGCAGGTCACCGAGGCGACC
TGGTACCGGTGGCTGAACCAGTACGGGTCCGAGAAGAACGCCGAGGCGACGAAACGGACCAAGGAGCTCGAGAAGGAGAACGCGAGGCTCAAGCGGCTGC
TGGCCGAGAAAGAGCTAGCCATCGACATCCTGAACGAGGTGGCCAAGGGAAAATTCTGAGCCCCGAACCACGCCGCCGTGCCGTGCGCATGGCGATGGAG
AAGTTCGGGGCGTCGGAACGCTTTGCCTGCAAAGTGCTGGGGCAGAACCGCTCCGCCCTGCGCAAGAAGAAACCCGAAATGAGTTTCGAGGAAACACGGC
TGCGCGCTGACCTGCGGGCAGTCGCGCAGAAACACCCGGCGTGGGGCTGGAAGAAGGCCCGCTGGCACCTGCGTGCCCAGCCGCAGTGGCAGGACGTGGC
GCTGAACAAGAAGCGGGTACGCCGGCTCTGGCGCGACGAGGGACTGGTTTGTAAACCCAAGCCGAAGAAGAAGCGCCGGACCGGCCCGGACGCCGGGGAA
CAGAAGCGCCTGAAAGCCGAATACCCGATGCACGTGATCAGCTTCGACTTCCAGTCCGACGTCACCTCCTGCGGGCGCCACATCCGCTTCTTCAACGTCA
TCGACGAATGCACCCGCACGGCGCTGGCGATCGTGCCGCGGCGCTCGTTCAAGGCTTCCGACGTGGTCGCCGTGCTGGAGAACATCATCGCCGAAACCGG
TATCGAACCGGCGTACGTGCGCTGCGACAACGGACCGGAATTCACCGCCGCCGCACTGATCGAATGGTGCAGCACAGCCGGTGTGAAGACCGCGTTCATC
GACCCGGGATCGCCCTGGCAGAACGGGTTCATCGAATCATTCAACGCCCAATTCAGAAGGGAACAACTCTCCGGAGAAATCATCGACACCATGGCCGAAG
CAAAGTACTTGGCCGACGAATGGAAAGACATCTACAATCATGAACGGCCCCACGGATCCCTGGATGGAATGACACCATCCAACTACTGGAATCAGTGGAC
CGCAGACCACCAATCAGCTATCGCATAGCCGCTGGACTGCTAAAGGGGGCCCAATCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 279 bp | 92 aa | 81 | 359 | + | No |
Description : First part of the transposase
ORF sequence :
MARRHTPEQVIAKVRQGQKMLNEGRPLIEVVKELQVTEATWYRWLNQYGSEKNAEATKRTKELEKENARLKRLLAEKELAIDILNEVAKGKF
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1080 bp | 359 aa | 149 | 1228 | + | No |
Description : Second part of the transposase
ORF sequence :
RTAPHRGRQGTAGHRGDLVPVAEPVRVREERRGDETDQGAREGEREAQAAAGRERASHRHPERGGQGKILSPEPRRRAVRMAMEKFGASERFACKVLGQN
RSALRKKKPEMSFEETRLRADLRAVAQKHPAWGWKKARWHLRAQPQWQDVALNKKRVRRLWRDEGLVCKPKPKKKRRTGPDAGEQKRLKAEYPMHVISFD
FQSDVTSCGRHIRFFNVIDECTRTALAIVPRRSFKASDVVAVLENIIAETGIEPAYVRCDNGPEFTAAALIEWCSTAGVKTAFIDPGSPWQNGFIESFNA
QFRREQLSGEIIDTMAEAKYLADEWKDIYNHERPHGSLDGMTPSNYWNQWTADHQSAIA
RSALRKKKPEMSFEETRLRADLRAVAQKHPAWGWKKARWHLRAQPQWQDVALNKKRVRRLWRDEGLVCKPKPKKKRRTGPDAGEQKRLKAEYPMHVISFD
FQSDVTSCGRHIRFFNVIDECTRTALAIVPRRSFKASDVVAVLENIIAETGIEPAYVRCDNGPEFTAAALIEWCSTAGVKTAFIDPGSPWQNGFIESFNA
QFRREQLSGEIIDTMAEAKYLADEWKDIYNHERPHGSLDGMTPSNYWNQWTADHQSAIA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1148 bp | 382 aa | 81 | 1228 | + | Yes |
Chemistry : DDE
ORF sequence :
MARRHTPEQVIAKVRQGQKMLNEGRPLIEVVKELQVTEATWYRWLNQYGSEKNAEATKRTKELEKENARLKRLLAEKELAIDILNEVAKGKILSPEPRRR
AVRMAMEKFGASERFACKVLGQNRSALRKKKPEMSFEETRLRADLRAVAQKHPAWGWKKARWHLRAQPQWQDVALNKKRVRRLWRDEGLVCKPKPKKKRR
TGPDAGEQKRLKAEYPMHVISFDFQSDVTSCGRHIRFFNVIDECTRTALAIVPRRSFKASDVVAVLENIIAETGIEPAYVRCDNGPEFTAAALIEWCSTA
GVKTAFIDPGSPWQNGFIESFNAQFRREQLSGEIIDTMAEAKYLADEWKDIYNHERPHGSLDGMTPSNYWNQWTADHQSAIA
AVRMAMEKFGASERFACKVLGQNRSALRKKKPEMSFEETRLRADLRAVAQKHPAWGWKKARWHLRAQPQWQDVALNKKRVRRLWRDEGLVCKPKPKKKRR
TGPDAGEQKRLKAEYPMHVISFDFQSDVTSCGRHIRFFNVIDECTRTALAIVPRRSFKASDVVAVLENIIAETGIEPAYVRCDNGPEFTAAALIEWCSTA
GVKTAFIDPGSPWQNGFIESFNAQFRREQLSGEIIDTMAEAKYLADEWKDIYNHERPHGSLDGMTPSNYWNQWTADHQSAIA
Blast result :
Comments
ISAau1 is 61% aa similar to ISDsp2. The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] ISfinder annotation (2009)
2] Mongodin,E.F., Shapir,N., Daugherty,S.C., Deboy,R.T., Emerson,J.B., Shvartzbeyn,A., Radune,D., Vamathevan,J., Riggs,F., Grinberg,V., Khouri,H., Wackett,L.P., Nelson,K.E. and Sadowsky,M.J. (2006) PLoS Genet. 2 (12), E214
2] Mongodin,E.F., Shapir,N., Daugherty,S.C., Deboy,R.T., Emerson,J.B., Shvartzbeyn,A., Radune,D., Vamathevan,J., Riggs,F., Grinberg,V., Khouri,H., Wackett,L.P., Nelson,K.E. and Sadowsky,M.J. (2006) PLoS Genet. 2 (12), E214