ISEfa10
- Family IS3
- Group IS150
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| FJ795373 | ND | Enterococcus faecium | Enterococcus faecium |
DNA section
IS Length : 1561 bp
Ends
IR Length : 22/24
IRL : TGTACCGACCCCCAAAAGTTAGACCAAAAAATCTAACTTTTGGAGGTCGG
IRR : TGTAGTGACCCCCAAAAGTTAGACTTTTTTGGAGTGGAGAAATCCACTCC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| ATTGATCACTTGTAAATCGGT | AGA | TGGTGGCTCCTTTTCCAGTTCCATAATGT | 3 |
DNA sequence
TGTACCGACCCCCAAAAGTTAGACCAAAAAATCTAACTTTTGGAGGTCGGTATTTTTTATGGCTAAGTATACTTTTGAACTTAAATTAAAGATCGTTCAT
GATTACTTGGATGGTAAAGGCGGAAGTGATTATCTGGCAAAGAAATATTCCATTAAGGCTCCATCACAAGTGAAGCGCTGGATCAATGCCTATCAAGAAT
TTGGTGAAGAGGGTCTAGTAAGAAAGCGACAAAAGAAAAAATATTCTGTTCAATTTAAGCTGGATGCGATAGAGTTATATCTAACAAGTGAGCTGTCCTA
TCGTGAAGTTGCCAACACACTTAACATGAACAACCCTAATTTGATCGCTAGTTGGATGCGTCAATTCCGTGAGGGTGGGATCGATGGACTCTCAAAAACG
AAAGGATGTCCACCTATCTTGTCTAAGAAAAATGAACCCAAAAATAAGAAAAAATCTACAACTAAAGCAACGTCTAAAGAACGTGAATGCATCGAGGAGT
TGGAGAAACGAGTCCGTTCCCTTCAAATTGAAAATGCCTTTTTAAAAGAATTGAGGAAGCTGCGAAAACAGGAAGCCCAACAACGACGAATGAAGCAATC
GCACGAATCATCGCAAGCCTCCGAGGACCGTTTAAATTAGTTGAGCTTCTAGAAACTTTAAAGTTCCCTAAGGCAACCTTTATGTACTGGCAAAAACGAT
TGGACCGTAAAAATCCAGATCAAGAGATTGAAGCGGAAATGCTTAAAGTTCGGGAGAAGCACAAGGATTATGGCTGTTTACGGATGACCAATGAACTAAG
AAACCGCGGTTTCTCTATCAATAAAAAGAAAGTTCAGCGCCTGATCAAGAAGCTAGGAATAAAAGTAACGACATACACACGTAAATCACGGCGCTACAAT
TCTTATCGAGGACAAGTCGGAGCAGTAGCCAAAAATCGGATCCATCGTCGTTTCTACACCAGTATCTGCCATCAGAAAATCACGACGGATACAACTGAAT
TTAAATACCATGAAGTGGATGCCAAAGGCATTATCCGTCAAAAGAAATTGTATCTAGATCCTTTTATGGATATGTTCAATAGTGAGATTTTATCCTATCG
TATCTCTGAAAAGCCCAATGCATTAGCGGTAATGGAAGGTTTGGAGGAAGCTATCCAGACCACTAACGATTGTCCCTATCGTCGTACGTTTCACTCAGAT
CAAGGTTGGGCGTATCAAATGAAAGCTTATAGAAACAAATTGAAGGAGTACAAAATCTTTCAAAGCATGTCTCGGAAAGGGAACTGCTTAGATAATTCAC
TTATGGAGAATTTTTTTGGCTTATTAAAACAAGAGATTTTTCACGGGAAAGTTTATAACTGTTTCGAGGAATTAAAATCAGCCATCGATAGCTATATCTA
TTACTACAATAATGAACGGATAAAACAGAAATTAAACTGGCAAAGTCCCGTCCAATTTCGTAAAACAACCGCAACGGTTGTGTAAAAATATCATTTTAGA
GCATGAAAAAAGGAGTGGATTTCTCCACTCCAAAAAAGTCTAACTTTTGGGGGTCACTACA
GATTACTTGGATGGTAAAGGCGGAAGTGATTATCTGGCAAAGAAATATTCCATTAAGGCTCCATCACAAGTGAAGCGCTGGATCAATGCCTATCAAGAAT
TTGGTGAAGAGGGTCTAGTAAGAAAGCGACAAAAGAAAAAATATTCTGTTCAATTTAAGCTGGATGCGATAGAGTTATATCTAACAAGTGAGCTGTCCTA
TCGTGAAGTTGCCAACACACTTAACATGAACAACCCTAATTTGATCGCTAGTTGGATGCGTCAATTCCGTGAGGGTGGGATCGATGGACTCTCAAAAACG
AAAGGATGTCCACCTATCTTGTCTAAGAAAAATGAACCCAAAAATAAGAAAAAATCTACAACTAAAGCAACGTCTAAAGAACGTGAATGCATCGAGGAGT
TGGAGAAACGAGTCCGTTCCCTTCAAATTGAAAATGCCTTTTTAAAAGAATTGAGGAAGCTGCGAAAACAGGAAGCCCAACAACGACGAATGAAGCAATC
GCACGAATCATCGCAAGCCTCCGAGGACCGTTTAAATTAGTTGAGCTTCTAGAAACTTTAAAGTTCCCTAAGGCAACCTTTATGTACTGGCAAAAACGAT
TGGACCGTAAAAATCCAGATCAAGAGATTGAAGCGGAAATGCTTAAAGTTCGGGAGAAGCACAAGGATTATGGCTGTTTACGGATGACCAATGAACTAAG
AAACCGCGGTTTCTCTATCAATAAAAAGAAAGTTCAGCGCCTGATCAAGAAGCTAGGAATAAAAGTAACGACATACACACGTAAATCACGGCGCTACAAT
TCTTATCGAGGACAAGTCGGAGCAGTAGCCAAAAATCGGATCCATCGTCGTTTCTACACCAGTATCTGCCATCAGAAAATCACGACGGATACAACTGAAT
TTAAATACCATGAAGTGGATGCCAAAGGCATTATCCGTCAAAAGAAATTGTATCTAGATCCTTTTATGGATATGTTCAATAGTGAGATTTTATCCTATCG
TATCTCTGAAAAGCCCAATGCATTAGCGGTAATGGAAGGTTTGGAGGAAGCTATCCAGACCACTAACGATTGTCCCTATCGTCGTACGTTTCACTCAGAT
CAAGGTTGGGCGTATCAAATGAAAGCTTATAGAAACAAATTGAAGGAGTACAAAATCTTTCAAAGCATGTCTCGGAAAGGGAACTGCTTAGATAATTCAC
TTATGGAGAATTTTTTTGGCTTATTAAAACAAGAGATTTTTCACGGGAAAGTTTATAACTGTTTCGAGGAATTAAAATCAGCCATCGATAGCTATATCTA
TTACTACAATAATGAACGGATAAAACAGAAATTAAACTGGCAAAGTCCCGTCCAATTTCGTAAAACAACCGCAACGGTTGTGTAAAAATATCATTTTAGA
GCATGAAAAAAGGAGTGGATTTCTCCACTCCAAAAAAGTCTAACTTTTGGGGGTCACTACA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 582 bp | 193 aa | 59 | 640 | + | No |
Description : First part of the transposase
ORF sequence :
MAKYTFELKLKIVHDYLDGKGGSDYLAKKYSIKAPSQVKRWINAYQEFGEEGLVRKRQKKKYSVQFKLDAIELYLTSELSYREVANTLNMNNPNLIASWM
RQFREGGIDGLSKTKGCPPILSKKNEPKNKKKSTTKATSKERECIEELEKRVRSLQIENAFLKELRKLRKQEAQQRRMKQSHESSQASEDRLN
RQFREGGIDGLSKTKGCPPILSKKNEPKNKKKSTTKATSKERECIEELEKRVRSLQIENAFLKELRKLRKQEAQQRRMKQSHESSQASEDRLN
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 954 bp | 317 aa | 532 | 1485 | + | No |
Description : Second part of the transposase
ORF sequence :
KCLFKRIEEAAKTGSPTTTNEAIARIIASLRGPFKLVELLETLKFPKATFMYWQKRLDRKNPDQEIEAEMLKVREKHKDYGCLRMTNELRNRGFSINKKK
VQRLIKKLGIKVTTYTRKSRRYNSYRGQVGAVAKNRIHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMDMFNSEILSYRISEKPNALAV
MEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLKEYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNERIKQK
LNWQSPVQFRKTTATVV
VQRLIKKLGIKVTTYTRKSRRYNSYRGQVGAVAKNRIHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMDMFNSEILSYRISEKPNALAV
MEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLKEYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNERIKQK
LNWQSPVQFRKTTATVV
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1427 bp | 475 aa | 59 | 1485 | + | Yes |
Chemistry : DDE
ORF sequence :
MAKYTFELKLKIVHDYLDGKGGSDYLAKKYSIKAPSQVKRWINAYQEFGEEGLVRKRQKKKYSVQFKLDAIELYLTSELSYREVANTLNMNNPNLIASWM
RQFREGGIDGLSKTKGCPPILSKKNEPKNKKKSTTKATSKERECIEELEKRVRSLQIENAFLKELRKLRKQEAQQRRMKQSHESSQASEDRLKLVELLET
LKFPKATFMYWQKRLDRKNPDQEIEAEMLKVREKHKDYGCLRMTNELRNRGFSINKKKVQRLIKKLGIKVTTYTRKSRRYNSYRGQVGAVAKNRIHRRFY
TSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMDMFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLKEYKI
FQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNERIKQKLNWQSPVQFRKTTATVV
RQFREGGIDGLSKTKGCPPILSKKNEPKNKKKSTTKATSKERECIEELEKRVRSLQIENAFLKELRKLRKQEAQQRRMKQSHESSQASEDRLKLVELLET
LKFPKATFMYWQKRLDRKNPDQEIEAEMLKVREKHKDYGCLRMTNELRNRGFSINKKKVQRLIKKLGIKVTTYTRKSRRYNSYRGQVGAVAKNRIHRRFY
TSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMDMFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLKEYKI
FQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNERIKQKLNWQSPVQFRKTTATVV
Blast result :
Comments
ISEfa10 is 56% aa similar to ISEnfa3.
The third ORF is a putative ORFAB trasposase reconstructed in silico by posible -1 frameshift.
The third ORF is a putative ORFAB trasposase reconstructed in silico by posible -1 frameshift.
References
1] Carmen Torres (2009) Direct submission.