ISAcma28

  • Family IS630
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_009925 ND Acaryochloris marina
Acaryochloris marina MBIC11017
DNA section
IS Length : 1130 bp

Ends


IR Length : 20/30

IRL : CCAATTCCATATATGTGTGACTTGTATTATGGGAGTAGCGCGTTGCTGAT
IRR : CCAATTCACTTATTGAGTGACCTTTATAGTCAAAGCATCCAAGATATAAG

Insertion site


Left flankDirect repeatRight flankDR Length
CCACCATCAAACCCAATCGAGTGATATGACACCGAGGATTGCCGACTTTA2
TTAACTCAGGCTATTCATGACTTATATTACGACTACTTTGACAAAGCATG2
AGCGTCCCTTGCAGACCCTTGTCATATATCAGGTTACAACACGACACACA2
GGGTTGACCCAAGAAATTTTCTAATATGACGCAGCATCAGAAACAGGTTG2
AGTCAGCTTTCTTCTTCTGAGTAATATAAGACCCATGAAACTCTAAACGA2

DNA sequence

CCAATTCCATATATGTGTGACTTGTATTATGGGAGTAGCGCGTTGCTGATACTTATCCCTCATGCCCAGACCCTTACGAATTGATATCTGTGAAAGTGAA
GACACACTCAAACGTTTATTACGTCAACAATCCTCCGGTCGTCGCAAAGAACGCTTGCAAGCCCTCTACTGGCTCAAAAGTGGTCAAGTCACCACTCGCA
TGGAGTTGGCAACCCTGACCGGACGAGGGGAATCCACGGTGTATCGCTGGATCAAACTCTATAAATCAGGGGGATTACAGGCAATGCTGGAGATTCCTAC
CTCTCCTGGGAAGCCCCCCATGATTGGTGGAGAGGTCCTCGAAAAGCTGAAAGCTCGTCTAGCTGACCCCAGGGGCTTTGAGAGCTACGGGGCGATTCAA
CAATGGCTTTTAGATGAGTGTGGATTAGACGTACCCTACAAAACGGTTCATCAAACCGTGCATTATCGGCTCAAAGCCAAGCTCAAAGTTCCTCGACCTC
GCTCACGCAAGACAGAGGAAGCTCAACAACAAGCCTATTAAAAAACTTCCTCGTGTGATTGAGGTAATGACCCGCTATTTTGCCCAAGGACACCCAGTGC
GCTACTGGTGCATGGATGAAAGCCGCTGGGGACTCAAGACACTCACGGGTCGGGTTCTAACGTTGCGGGGTGTCAAACCTGTGGTGACGGTACAGTGGCC
CCGAGAGGCTTTCTGGCTCTATGGCGCTGTCGAACCTCTCACAGGAGACAGTTTCTTCTATCGGTTTTCTCATCTCGATAGTGTTTGTTTTGAGCGCTTC
CTGGAGCAATTCAGCTTGGCGTTTCCCCAGAGTCTCAATCTGATGCAAATAGATCAAGCCGCAGCCCATATGGCGACTTGGGTTCGATGGCCTGAGAATG
TGTTGCCGATTGTACAGCCGTCTCATAGTCCAGAACTCAATCCGGCAGAGCGATTATGGCAGGACCTAAGAAAGCCTTTCAAAGGCAAAAACTTTGATTC
TATTGGGGCACTGGAGACCGCTTTATTTGAGCATATCAATACCCTCTCCCAGAAGGTGGTGGCTTCTCTAACGGGATATTCTTATATCTTGGATGCTTTG
ACTATAAAGGTCACTCAATAAGTGAATTGG
Recoding section
  • Recoding by frameshift
  • Frame
  • Type
  • Experimentally demonstrated

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
480 bp159 aa62541+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MPRPLRIDICESEDTLKRLLRQQSSGRRKERLQALYWLKSGQVTTRMELATLTGRGESTVYRWIKLYKSGGLQAMLEIPTSPGKPPMIGGEVLEKLKARL
ADPRGFESYGAIQQWLLDECGLDVPYKTVHQTVHYRLKAKLKVPRPRSRKTEEAQQQAY

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
555 bp184 aa5671121+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

MTRYFAQGHPVRYWCMDESRWGLKTLTGRVLTLRGVKPVVTVQWPREAFWLYGAVEPLTGDSFFYRFSHLDSVCFERFLEQFSLAFPQSLNLMQIDQAAA
HMATWVRWPENVLPIVQPSHSPELNPAERLWQDLRKPFKGKNFDSIGALETALFEHINTLSQKVVASLTGYSYILDALTIKVTQ

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1060 bp349 aa621121+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MPRPLRIDICESEDTLKRLLRQQSSGRRKERLQALYWLKSGQVTTRMELATLTGRGESTVYRWIKLYKSGGLQAMLEIPTSPGKPPMIGGEVLEKLKARL
ADPRGFESYGAIQQWLLDECGLDVPYKTVHQTVHYRLKAKLKVPRPRSRKTEEAQQQAYKKLPRVIEVMTRYFAQGHPVRYWCMDESRWGLKTLTGRVLT
LRGVKPVVTVQWPREAFWLYGAVEPLTGDSFFYRFSHLDSVCFERFLEQFSLAFPQSLNLMQIDQAAAHMATWVRWPENVLPIVQPSHSPELNPAERLWQ
DLRKPFKGKNFDSIGALETALFEHINTLSQKVVASLTGYSYILDALTIKVTQ

 

Blast result :
Comments
ISAcma28 is 50% aa similar to ISSpn2. The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Swingley,W.D., Chen,M., Cheung,P.C., Conrad,A.L., Dejesa,L.C.,Hao,J., Honchak,B.M., Karbach,L.E., Kurdoglu,A., Lahiri,S.,Mastrian,S.D., Miyashita,H., Page,L., Ramakrishna,P., Satoh,S., Sattley,W.M., Shimada,Y., Taylor,H.L., Tomo,T., Tsuchiya,T., Wang,Z.T., Raymond,J., Mimuro,M., Blankenship,R.E. and Touchman,J.W. (2008) Proc. Natl. Acad. Sci. U.S.A.
2] ISfinder annotation (2009)