ISfinder Mirror Site

ISAcma27

Family IS630
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_009925	ND	Acaryochloris marina	Acaryochloris marina MBIC11017

DNA section

IS Length : 1185 bp

Ends

IR Length : 17/19

IRL : AGTAGCCCAGCCTTGAAAAGCTCAATTAGATGAAGTTGAATGAATCTACC
IRR : AGTAGCCAAGCCTTAAAAATTGTCTGTACGTAAAGGCTCATGAAGCAG

Insertion site

Left flank	Direct repeat	Right flank	DR Length
AACCACCGACGAATACGACGA	TA	CCACCAGTAGAAAGCTATTT	2
GCAGTTTACCCTAGCGCCAGG	TA	GAACTCTGATTCTCCTGGAT	2

DNA sequence

AGTAGCCCAGCCTTGAAAAGCTCAATTAGATGAAGTTGAATGAATCTACCCACACTATCGTTTAGGAGATTGTGAGGATGGGTGCCCGATTACGTACTTT
ATTGAATAGCTCAGAAGAACAGACCTTATTTGAACTGAGGACAGCAACAACAGTCCCCCAACGGGTCAAAGATCGAGCCGCAATGATTCGATTGAGTCAT
CAGGGGATGTATGTAGAGAAAATTGCAGCCCTATTCCAGTGTAATGTTCGGACCGTTCGCCAAACCCTTCATCGCTGGCAACAGAAGGGATTAGGAGGGC
TATGGGATGCACCACATCCGGGTGCTCAGCGACGTTGGAACCCCGAGGATATCGAGTATCTTGAAACCTGTTTGCGTCAGGAAGAACGCACCTACAATAG
CCAACAACTGTCCCAGAAACTGGCGAAGGAGCGTAACGTTCACTTAAGTGCAGACCGCATTAGGCAGATTCTCAAAAAAAGGGGGTGATCTGGAAACGAA
CTCGTCATAGTCACCGCAATAAGCAAGACCCTGTTAAGAAAGCTATCAAACTCGCTGATTTAGAGATGTTGCAGTGGGCAGCAGCGAGTGGAGAGATTGA
GTTGTATTACCTGGATGAGTCTGGTTTTTGCCTATGGATGCCCGTGGAATATAGCTATTTCTTCAAGGGAGAGCAAAAGCGGATGGAACAGACTAAACGT
AAGGGAAGACGTATCAGTATTTTGGGGCTTCTCCAACCGTTGACGACCTTTGTTTATGGGCTAGTTGTCGGCAGTTTTAATGCCGACCGTTACATCAAAA
TAATGGATGAACAAGCATGCCAGGCCCAACAACAACTGGATAAAACTGGACGAATTCGGGTCATCGTTCAAGACAATGGCTCTAGTCATACTTGCAACAA
AGCGAAGCAAAAATGGACACAGTGGCAAACCCAAGGGCTCTATTTGTTCTTTCTGCCCAAATACTGTTCACAGATGAATCCGAGAAACAGAGTGGCACCA
GCTCAAAGCGCACCAATTGAGGGGGCAAATGTTTGAGCATGAGCTGGATTTAGCCTATGCGGTGATGGATGGCGTGAAGGCTAGAGCGTAAGCGGGAAAC
TATGAAGCAAAACGGTTGAGATTCCCATCACGGCTCGCTGCTTCATGAGCCTTTACGTACAGACAATTTTTAAGGCTTGGCTACT

Recoding section

Recoding by frameshift
Frame
Type
Experimentally demonstrated

Stimulators :

Shine-Dalgarno sequence :
Secondary structure :

Recoding motif :

Protein section

ORF number : 3

ORF 1

Length		Begin	End	Strand	Fusion ORF
411 bp	136 aa	78	488	+	No

ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MGARLRTLLNSSEEQTLFELRTATTVPQRVKDRAAMIRLSHQGMYVEKIAALFQCNVRTVRQTLHRWQQKGLGGLWDAPHPGAQRRWNPEDIEYLETCLR
QEERTYNSQQLSQKLAKERNVHLSADRIRQILKKRG

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
552 bp	183 aa	485	1036	+	No

ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

MIWKRTRHSHRNKQDPVKKAIKLADLEMLQWAAASGEIELYYLDESGFCLWMPVEYSYFFKGEQKRMEQTKRKGRRISILGLLQPLTTFVYGLVVGSFNA
DRYIKIMDEQACQAQQQLDKTGRIRVIVQDNGSSHTCNKAKQKWTQWQTQGLYLFFLPKYCSQMNPRNRVAPAQSAPIEGANV

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
959 bp	318 aa	78	1036	+	Yes

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MGARLRTLLNSSEEQTLFELRTATTVPQRVKDRAAMIRLSHQGMYVEKIAALFQCNVRTVRQTLHRWQQKGLGGLWDAPHPGAQRRWNPEDIEYLETCLR
QEERTYNSQQLSQKLAKERNVHLSADRIRQILKKGVIWKRTRHSHRNKQDPVKKAIKLADLEMLQWAAASGEIELYYLDESGFCLWMPVEYSYFFKGEQK
RMEQTKRKGRRISILGLLQPLTTFVYGLVVGSFNADRYIKIMDEQACQAQQQLDKTGRIRVIVQDNGSSHTCNKAKQKWTQWQTQGLYLFFLPKYCSQMN
PRNRVAPAQSAPIEGANV

Blast result :

Comments

ISAcma27 is 49% aa similar to ISTth6. The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.

References

1] ISfinder annotation (2009)
2] Swingley,W.D., Chen,M., Cheung,P.C., Conrad,A.L., Dejesa,L.C., Hao,J., Honchak,B.M., Karbach,L.E., Kurdoglu,A., Lahiri,S., Mastrian,S.D., Miyashita,H., Page,L., Ramakrishna,P., Satoh,S., Sattley,W.M., Shimada,Y., Taylor,H.L., Tomo,T., Tsuchiya,T., Wang,Z.T., Raymond,J., Mimuro,M., Blankenship,R.E. and Touchman,J.W.(2008) Proc. Natl. Acad. Sci. U.S.A.