ISfinder Mirror Site

ISAcma25

Family IS630
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_009925	ND	Acaryochloris marina	Acaryochloris marina MBIC11017

DNA section

IS Length : 950 bp

Ends

IR Length : 18/24

IRL : GCAGTCGCCATATTGAGAAGGACTTAAACTAGAGAAGTATTTTATTCCTT
IRR : GCAGTCGCCATTCTATTCAGGACACTAGATGTAGAACATGTTCCATTGCT

Insertion site

Left flank	Direct repeat	Right flank	DR Length
ACATAGGTCTGCAAAGTACTCAGAGA	TATA	TCTTCTGTTTTAGCCTGTGGGCTC	4
GCTAAAACGACTGTCCATGGAGAAGT	TATA	ACCCTCTTTTATCACTTTAGACAG	4
GAAGCTATTGGTCGACATCATGCCGA	TATA	CCATCCTTTTGAAGGATCGTCGTT	4
TATATCGTCAAAATAGACATTACACA	TATA	TATACATAAAAGCTTTGTAGATCA	4
AAAGCAAAGATAGAAGTTCTCATAAG	TATA	CTTCCTAAGGCTGTC	4

DNA sequence

GCAGTCGCCATATTGAGAAGGACTTAAACTAGAGAAGTATTTTATTCCTTCTCCTGTGTGATGCCCAAAATTTATAGTTACGACCTACGTTGCAAAGTGA
TAGATGCGATTGAGCTAGATGGGATGCGCCCTTCTGAGGCTAGTGAGCTGTTCGATATCAGTCGTAACACCATTAACCAATGGCAACACCTCAAGGCGGA
AACGGGTGATTTGCATCCCAAGCCAGTCCATCATCCTGGCCATAGCCATAAAATCACTGACTGGGACAAGTTTCGAGCGTTTGCCCACAAGCATCGTCAT
AAAACCCAAGCTCAGATGGCTCAACTATGGGATGGAGAGATTAGTGATCGAACCATCTCAAGAGCCCTCCAGAAAATCGGATTTACTCGAAAAAAAAACA
TATGGGTACCGAGAACGAGATAAGCACAAACGGGCAGCGTTCATCAAACGTCTGAGTACCGTGGACCCAGACGATATCGTCTATGCGGATGAGTCGGGCA
TGGATCATCGAGACGAGTATGACTATGCTTATGGCCCCAAAGGTGAGCGGGTTTATGCTTTGAAGACTGGGCGTAGAAGCGGCCGAGTCAATATGATTGC
GGCGTTGAGAGCAAAGCAGTTGATGGCACCGTTCACGATCGAAGGAGCTTGCAATCGGACTGTGTTTGAACTTTGGCTAGAGCGTTGCTTGATACCCGTG
CTCAAGCCTGGGCAGAAGCTGGTGATAGATAATGCCACTTTTCACAAGGGAGGACAAATACAGGAATTAGTGGAAAAAGCAGGATGTGAGGTTTGGTATT
TACCGCCTTATTCCCCTGACTTGAACAAGATTGAGCGGTCTTGGTCTTGGATTAAAAGTCGCATCCGTCACCAATTAGAACATTTTGGATCTCTCAGAGA
AGCAATGGAACATGTTCTACATCTAGTGTCCTGAATAGAATGGCGACTGC

Recoding section

Recoding by frameshift
Frame
Type
Experimentally demonstrated

Stimulators :

Shine-Dalgarno sequence :
Secondary structure :

Recoding motif :

Protein section

ORF number : 3

ORF 1

Length		Begin	End	Strand	Fusion ORF
363 bp	120 aa	61	423	+	No

ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MPKIYSYDLRCKVIDAIELDGMRPSEASELFDISRNTINQWQHLKAETGDLHPKPVHHPGHSHKITDWDKFRAFAHKHRHKTQAQMAQLWDGEISDRTIS
RALQKIGFTRKKNIWVPRTR

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
816 bp	271 aa	119	934	+	No

ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

MGCALLRLVSCSISVVTPLTNGNTSRRKRVICIPSQSIILAIAIKSLTGTSFERLPTSIVIKPKLRWLNYGMERLVIEPSQEPSRKSDLLEKKTYGYRER
DKHKRAAFIKRLSTVDPDDIVYADESGMDHRDEYDYAYGPKGERVYALKTGRRSGRVNMIAALRAKQLMAPFTIEGACNRTVFELWLERCLIPVLKPGQK
LVIDNATFHKGGQIQELVEKAGCEVWYLPPYSPDLNKIERSWSWIKSRIRHQLEHFGSLREAMEHVLHLVS

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
874 bp	290 aa	61	934	+	Yes

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MPKIYSYDLRCKVIDAIELDGMRPSEASELFDISRNTINQWQHLKAETGDLHPKPVHHPGHSHKITDWDKFRAFAHKHRHKTQAQMAQLWDGEISDRTIS
RALQKIGFTRKKTYGYRERDKHKRAAFIKRLSTVDPDDIVYADESGMDHRDEYDYAYGPKGERVYALKTGRRSGRVNMIAALRAKQLMAPFTIEGACNRT
VFELWLERCLIPVLKPGQKLVIDNATFHKGGQIQELVEKAGCEVWYLPPYSPDLNKIERSWSWIKSRIRHQLEHFGSLREAMEHVLHLVS

Blast result :

Comments

ISAcma25 is 57% aa similar to ISOt3.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.

References

1] Swingley,W.D., Chen,M., Cheung,P.C., Conrad,A.L., Dejesa,L.C.,Hao,J., Honchak,B.M., Karbach,L.E., Kurdoglu,A., Lahiri,S.,Mastrian,S.D., Miyashita,H., Page,L., Ramakrishna,P., Satoh,S., Sattley,W.M., Shimada,Y., Taylor,H.L., Tomo,T., Tsuchiya,T., Wang,Z.T., Raymond,J., Mimuro,M., Blankenship,R.E. and Touchman,J.W. (2008) Proc. Natl. Acad. Sci. U.S.A.
2] ISfinder annotation (2009)