ISMex8

  • Family IS21
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Methylobacterium extorquens
Methylobacterium extorquens AM1
DNA section
IS Length : 2597 bp

Ends


IR Length : 10/12

IRL : TGTGTAGGGCGGTGACAAAACCATCCACTGGAGCGTCGGCGGCGTGCTGA
IRR : TGTAAAGGGCGGACCAATTCCAGGCCGCTGTGGCGGAGTAAAACCAGGCC

Insertion site


Left flankDirect repeatRight flankDR Length
CTCCGGCGTCCCCTCGGCTT0
TGGTCGAAAGGGCGTCATCCTT2
CTGAGCGAGCGGCGGTTTCG0

DNA sequence

TGTGTAGGGCGGTGACAAAACCATCCACTGGAGCGTCGGCGGCGTGCTGATGCGGGCGGCGTAAAAGTCGGCCAGTTTGAGAGGCTTCTCTTTCGGGAGG
GGCCCAGGATGTTTGCCGTGGAAGTCTACGCGGCCGTTCGGCAGTTTGTTTTCATCGACGGCCACTCTCGTCGTGAGGCAGCCCGGGTATTTGGGCTCAA
TCGCGAGACGATTGCCAAGATGTGCCGGTTCTCGCTGCCGCCGGGCTACACGCGCTCGAAGCCGATCGAGAAGCCGAAGCTGGGGCCGCTGCTGCCGGTG
ATCGATGCGATCCTGGAGGCGGACCGGATCGCACCCGTCAAGCAGCGTCACACGGCCAAGCGGATTTTCGAGCGTCTGCGCGACGAGCACGGCTATGGCG
GCGGCTACACGGTGGTGAAGGACCACGTCCGGATCGCACGCGCGAGCGGGAGGGAGACCTTCGTGCCGTTGGCGCACCCACCCGGCCACGCGCAGGTCGA
CTTCGGCGAAGCGATCGCCACCATCGGCGGCGTGCGCCGGAAGATCCACTTCTTCTGCATGGACCTGCCGCATTCCGACGCCTGCTTCGTGAAGGCGTAT
CCGCGGGAGACCACCGAGGCGTTCCTCGACGGGCACGTCGCCGCCTTCGATTTCTTCGCCGGCGTGCCGCTGTCGATTCTCTACGACAACACCAGGATCG
CGGTGGCCAAGATCTGCGGCGGAGGCCAGCGCGAACGTACCCGTGCCTTCACCGAGCTGGTTAGCCACTACCTGTTCCGAGATCGCTTCGGACGTCCCGG
CAGGGGCAACGACAAGGGCAAGGTCGAGGGGCTGGTCAAGTACGCCCGATCCAACTTCATGACCCCGGCCCCGGAGGCGGCCTCCTTCGAGGCGCTGAAC
GCGGATCTGGAGCGCCGCTGCCGGACCCGGCAGGATGAGCGGGCCGGACAGCATGCCGAGAGCATCGGCACGCGCTTGGTCGCCGATCGTGCCGTCCTGC
GTGCCCTGCCGGCGGTCCCGCTGGAGCCGTGCGAGAAGCGGGGTGGGCGGGTCTCCTCGACCGCGCTGGTTCGCTACCGCTGCAACGACTACTCGGTGCC
GACCGCCTACGGCTTCCGGGACGTGCTGGTGAAGGGCTTCGTCGAGGAGGTCGTGATCCTATGCGGCGGCATCGAGATCGCGCGGCATGAGCGCAGCTAC
GGCACCGGCGTGTTCGTGTCCGACCCGCTGCATTACCTCGCGCTGATCGAGATCAAACCGAACGCTCTCGACCAGGCGGCCGCGCTCCAGGGCTGGGATC
TGCCCGAGGCGTTCCAGCACCTACGCCACCTCCTGGAGGCGCGCATGGGTAACCGCGGCAAGCGGGAGTTCATCCAGGTGCTGCGCCTGATGGAGGCGAT
GCCCAAGGACGTCGTGGCCTCGTCCGTCACCGACGCGATCCGCCTCGGTGCGATCGGCTTCGACGCGGTCAAGCTGATCGCGCTGGCCCGGCTCGAACGA
CGACCGCCCCGGCTCGACCTGGCGGCCTACCCCCATCTGCCGAGGATGACGGTGAGGACGACCGCGGCCGCCGACTACACGGTGTTGGTGCCGGAGGTGG
CAGCATGAGCGCGGCACGTGACGAGACGATGCCGGGCGGCACCACCGGCGGCACGCCGGGGATCCTGCTCGCCCACCACCTCAAGCAGCTGAAGCTGCCC
ACGGTGCTGCGCGAGTACGACAAGGTCGCCCGGGAGTGCGCCCAGGGCGGCATCGACCACCCGCGCTACCTGCTGCGGCTCGTCGAGCTGGAACTGATCG
ATCGCGAACGGCGCATGGTCGAACGCCGGATCCGGTCGGCACGCTTCCCGGCGGTGAAGAGCCTCGACACCTTCGACTTCACCGCGATCCCGAGCCTGAA
CAAGATGCTCGTGCTGGAGCTGGCGCGCTGCGGCTACATCCTTGGCCGAGAGAACGTCATCGCACTCGGCAACTCCGGCACCGGCAAGACCCACATCGCC
TTGGCTCTCGGCCTGGCAGCTTGCCAGAAGGGCTTCTCGGTCGCGTTCACCACCGCGGCCTCTCTGGTCAACCAGCTCCTGGAGGCACGCGACGAGCGCC
GCCTGCTCCGGCTGCAGCGCGAACTGGCTTCGGTGAAGCTGCTGATCGTCGACGAACTCGGCTACGTGCCGCTGTCGGCCACAGGAGCCGAGTTGCTGTT
CGAGACCTTCTCGCAACGCTACGAGCGCGGCTCGACCGTGGTGACCTCGAACCTGCCGTTCGAGGATTGGACCTCGGTCCTGGGCTCGGAGCGGCTCACC
GGCGCGCTGCTCGATCGGCTGACCCACCACGTCAGCATCCTCAGCCTGAACGGCGACAGCTACCGCCTCAAGGCCTCCCGCAGCCGACGCGGCTGCGGCG
ATGGCAGGGCGGAGCAAAACCAGGCCACCATCGATCCCCACGATCCCGAGACGGGCGAGATCCTGCCGGCTTGAGCCTCAGGATCGGCGACGAACGATGA
AGGGGCCCGATCGGGCCCCTTCATCGTTCAAGCTGCCGGCCGCCACTGGCCTGGTTTTACTCCGCCACAGCGGCCTGGAATTGGTCCGCCCTTTACA
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
1500 bp499 aa1091608+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MFAVEVYAAVRQFVFIDGHSRREAARVFGLNRETIAKMCRFSLPPGYTRSKPIEKPKLGPLLPVIDAILEADRIAPVKQRHTAKRIFERLRDEHGYGGGY
TVVKDHVRIARASGRETFVPLAHPPGHAQVDFGEAIATIGGVRRKIHFFCMDLPHSDACFVKAYPRETTEAFLDGHVAAFDFFAGVPLSILYDNTRIAVA
KICGGGQRERTRAFTELVSHYLFRDRFGRPGRGNDKGKVEGLVKYARSNFMTPAPEAASFEALNADLERRCRTRQDERAGQHAESIGTRLVADRAVLRAL
PAVPLEPCEKRGGRVSSTALVRYRCNDYSVPTAYGFRDVLVKGFVEEVVILCGGIEIARHERSYGTGVFVSDPLHYLALIEIKPNALDQAAALQGWDLPE
AFQHLRHLLEARMGNRGKREFIQVLRLMEAMPKDVVASSVTDAIRLGAIGFDAVKLIALARLERRPPRLDLAAYPHLPRMTVRTTAAADYTVLVPEVAA

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
870 bp289 aa16052474+No
ORF function : Accessory Gene
AG : IS21 helper

ORF sequence :

MSAARDETMPGGTTGGTPGILLAHHLKQLKLPTVLREYDKVARECAQGGIDHPRYLLRLVELELIDRERRMVERRIRSARFPAVKSLDTFDFTAIPSLNK
MLVLELARCGYILGRENVIALGNSGTGKTHIALALGLAACQKGFSVAFTTAASLVNQLLEARDERRLLRLQRELASVKLLIVDELGYVPLSATGAELLFE
TFSQRYERGSTVVTSNLPFEDWTSVLGSERLTGALLDRLTHHVSILSLNGDSYRLKASRSRRGCGDGRAEQNQATIDPHDPETGEILPA

 

Blast result :
Comments
ISMex8 is 69% (ORFA) aa similar to ISFlsp1 and 86% (ORFB) to ISSsp5.
References