ISPpu21
- Family IS5
- Group IS5
Isoform Synonym(s) ISPpuS12
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| FJ905320 | Y | Pseudomonas putida | Pseudomonas putida S12 Pseudomonas aeruginosa W368 Pseudomonas aeruginosa serotype 015-ATCC |
DNA section
IS Length : 1190 bp
Ends
IR Length : 11/18
IRL : GGATGGTCTGAAAAAGACTTCCTGATTTGGCAAAATCCGCCGACTTCACC
IRR : GAAACTCTGAAGTAGCCACGTATTTCTGGCCGACTACAGACCTCGCTGTT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CGCCTTTCG | CCTTA | GGGTATTGGA | 5 |
| GTCCAGATCG | CTAG | CAAAACCACT | 4 |
DNA sequence
GGATGGTCTGAAAAAGACTTCCTGATTTGGCAAAATCCGCCGACTTCACCCGCAGAGCTTTCCGATGAAGCAGATGACCTTCGCCGACGCCGAGTACGCC
GGCAAGCGCAAGCAGACCCGCAAGGAACTGTTCCTGATCGAAATGGAACAGGTGGTGCCGTGGCAGGGCCTGATCGCCCTGATCGAGCCGCACTACCCGA
AGGGTGAAGGCGGTCGTCCCGCCTATCCGCTGATGGCGATGCTGCGCGTGCATCTGTTGCAGAACTGGTTCGGCTACAGCGACCCGGCGATGGAGGAAGC
GCTGTACGAGACGACCATCCTGCGCCAGTTCGCCGGGCTGAGCCTGGAGCGTATCCCCGACGAAACCACCATCCTCAACTTCCGTCGCCTGCTGGAGCGC
CACGAGCTGGCGGCCGGGATTCTGGCGGTGATCAATGGCTACCTGGGCGACCGTGGCCTGTCGCTGCGCCAGGGCACCATCGTCGACGCCACGCTGATCC
ATGCGCCGAGCTCGACCAAGAACAAGGACGGCAAGCGCGATCCCGAGATGCACCAGACCAAGAAGGGCAACCAGTACTACTTCGGCATGAAGGCGCACAT
CGGCGCCGACAGCGAATCCGGCCTGGTACACAGCGTGGTGGGCACGGCGGCCAACGTGGCCGATGTCACCCAGGTGGACAAGCTGCTGCACGGCGAAGAG
AACCTGGTCAGCGGCGATGCCGGCTATACCGGCGTCGAGAAGCGTGCCGAGCATGCAGGCCGCTCGGTCATCTGGCAGATTGCGGCGCGGCGCAGCACTT
ACCAGCAACTGAGCAAGCGCAGCCCGCTGTACAAGGCCAAGCGCAAAATCGAGAAGGCCAAGGCGCAGGTACGTGCCAAGGTCGAACATCCATTCCGGGT
GATCAAGCGCCAGTTTGGTTACACCAAGGTGCGCTTCCGTGGCCTGGCCAAGAACACCGCACAACTGATTACGCTGTTTGCGCTGTCGAATCTGTGGATG
GCGCGGCGGCATTTACTCGCGAACACAGGAGAGGTGCGCCTGTAACACGGCGAATAGCCGCTACGGGGTACGCGTAGCGGCTAAAAACCCTACATAAGAC
GACGATCTGAGCGCTTTTTGATCGATGGATCGCTTCAAAAAACAGCGAGGTCTGTAGTCGGCCAGAAATACGTGGCTACTTCAGAGTTTC
GGCAAGCGCAAGCAGACCCGCAAGGAACTGTTCCTGATCGAAATGGAACAGGTGGTGCCGTGGCAGGGCCTGATCGCCCTGATCGAGCCGCACTACCCGA
AGGGTGAAGGCGGTCGTCCCGCCTATCCGCTGATGGCGATGCTGCGCGTGCATCTGTTGCAGAACTGGTTCGGCTACAGCGACCCGGCGATGGAGGAAGC
GCTGTACGAGACGACCATCCTGCGCCAGTTCGCCGGGCTGAGCCTGGAGCGTATCCCCGACGAAACCACCATCCTCAACTTCCGTCGCCTGCTGGAGCGC
CACGAGCTGGCGGCCGGGATTCTGGCGGTGATCAATGGCTACCTGGGCGACCGTGGCCTGTCGCTGCGCCAGGGCACCATCGTCGACGCCACGCTGATCC
ATGCGCCGAGCTCGACCAAGAACAAGGACGGCAAGCGCGATCCCGAGATGCACCAGACCAAGAAGGGCAACCAGTACTACTTCGGCATGAAGGCGCACAT
CGGCGCCGACAGCGAATCCGGCCTGGTACACAGCGTGGTGGGCACGGCGGCCAACGTGGCCGATGTCACCCAGGTGGACAAGCTGCTGCACGGCGAAGAG
AACCTGGTCAGCGGCGATGCCGGCTATACCGGCGTCGAGAAGCGTGCCGAGCATGCAGGCCGCTCGGTCATCTGGCAGATTGCGGCGCGGCGCAGCACTT
ACCAGCAACTGAGCAAGCGCAGCCCGCTGTACAAGGCCAAGCGCAAAATCGAGAAGGCCAAGGCGCAGGTACGTGCCAAGGTCGAACATCCATTCCGGGT
GATCAAGCGCCAGTTTGGTTACACCAAGGTGCGCTTCCGTGGCCTGGCCAAGAACACCGCACAACTGATTACGCTGTTTGCGCTGTCGAATCTGTGGATG
GCGCGGCGGCATTTACTCGCGAACACAGGAGAGGTGCGCCTGTAACACGGCGAATAGCCGCTACGGGGTACGCGTAGCGGCTAAAAACCCTACATAAGAC
GACGATCTGAGCGCTTTTTGATCGATGGATCGCTTCAAAAAACAGCGAGGTCTGTAGTCGGCCAGAAATACGTGGCTACTTCAGAGTTTC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 981 bp | 326 aa | 65 | 1045 | + | No |
Chemistry : DDE
ORF sequence :
MKQMTFADAEYAGKRKQTRKELFLIEMEQVVPWQGLIALIEPHYPKGEGGRPAYPLMAMLRVHLLQNWFGYSDPAMEEALYETTILRQFAGLSLERIPDE
TTILNFRRLLERHELAAGILAVINGYLGDRGLSLRQGTIVDATLIHAPSSTKNKDGKRDPEMHQTKKGNQYYFGMKAHIGADSESGLVHSVVGTAANVAD
VTQVDKLLHGEENLVSGDAGYTGVEKRAEHAGRSVIWQIAARRSTYQQLSKRSPLYKAKRKIEKAKAQVRAKVEHPFRVIKRQFGYTKVRFRGLAKNTAQ
LITLFALSNLWMARRHLLANTGEVRL
TTILNFRRLLERHELAAGILAVINGYLGDRGLSLRQGTIVDATLIHAPSSTKNKDGKRDPEMHQTKKGNQYYFGMKAHIGADSESGLVHSVVGTAANVAD
VTQVDKLLHGEENLVSGDAGYTGVEKRAEHAGRSVIWQIAARRSTYQQLSKRSPLYKAKRKIEKAKAQVRAKVEHPFRVIKRQFGYTKVRFRGLAKNTAQ
LITLFALSNLWMARRHLLANTGEVRL
Blast result :
Comments
ISPpu21 is 97% aa similar to ISThi1.
Others accession number : AF498406 - JX440360.
ISPpu21 element was found truncating the oprD gene of the Pseudomonas aeruginosa W368.
Others accession number : AF498406 - JX440360.
ISPpu21 element was found truncating the oprD gene of the Pseudomonas aeruginosa W368.
References