ISAcma20

  • Family IS5
  • Group IS1031
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_009925 ND Acaryochloris marina
Acaryochloris marina MBIC11017
DNA section
IS Length : 867 bp

Ends


IR Length : 15/16

IRL : GAGCGTGTTTGAAAAGTCATCCAGGTAGAGTTGAGAGGCTCAATACATTA
IRR : GAGCGTGTTTGAGAAGATTTAGGCCAACCTTTTGAGCATGAGATTGACCA

Insertion site


Left flankDirect repeatRight flankDR Length
CAAACAGGTAGCTACCAGAAAAGTCCTTAGAGGCTGTTTGAGAAGTTTTAGTACT3

DNA sequence

GAGCGTGTTTGAAAAGTCATCCAGGTAGAGTTGAGAGGCTCAATACATTAGCCACTCAACCTCAAAATGGTACGACACCCCTACGATACAGACCTCACCC
TTGACCAGTGGGAACTGCTGCGTCCACTCTTGCCAAAGGCTAAGTCGGGTGGAAGACCTCGAAGGGTGAACTTGTTGGCCGTGGTCAATGCCATTTTCTA
CCTCCTACGTTCGGGGTGTCCCTGGCGACTGATACCCCATGACTTTCCAGCATGGCAAACGGTCTATACCTATTTCAGACAGTGGGAAGCCGATGGAACC
TGGGAGCGCCTCAATCGTGCCTTGCGAGAGAAACTCAGAATCCAAGCTGGGCGACATCGACATCCCAGTGCTGCTTGTGTTGATTCTCAATCCGTCAAAA
CAGCAGGGGCTGCCCAGGAAAAAGGCTTCGATGGCGGTAAGAAGGTGAAAGGGCGGAAGCGGACAATCCTCGTCGATACGATGGGATTACTCATCGGTGC
AACGGTTCACAGTGCCCGCCGCTCAGATCATCAAGGCATGATATTGCTAGGACTTTGGTTTTCAGCCTTTTGGCAAAGCATTCAGCTCATCTGGACCGAC
CAGACTTTTGGTGGTCAAGCCTTCACGGCTTGGTTTCAGAAGACTTTTGGCTGGACGATGGAAGTGGTCAAACGTCCGGAGGAGCAGCAAGGGTTTGAGG
TACTCCCTCGTCGTTGGGTGGTAGAGCGCACATTTGCTTGGTTTGGTCATTACCGTCGCTTGAGCAAAGATTATGAGTATTTACCAACAACGAGTGAGAT
GATGTTGTACGCTGCGATGGTCAATCTCATGCTCAAAAGGTTGGCCTAAATCTTCTCAAACACGCTC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
783 bp260 aa67849+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MVRHPYDTDLTLDQWELLRPLLPKAKSGGRPRRVNLLAVVNAIFYLLRSGCPWRLIPHDFPAWQTVYTYFRQWEADGTWERLNRALREKLRIQAGRHRHP
SAACVDSQSVKTAGAAQEKGFDGGKKVKGRKRTILVDTMGLLIGATVHSARRSDHQGMILLGLWFSAFWQSIQLIWTDQTFGGQAFTAWFQKTFGWTMEV
VKRPEEQQGFEVLPRRWVVERTFAWFGHYRRLSKDYEYLPTTSEMMLYAAMVNLMLKRLA

 

Blast result :
Comments
ISAcma20 is 80% aa similar to IS5Sa.
References
1] Swingley,W.D., Chen,M., Cheung,P.C., Conrad,A.L., Dejesa,L.C.,Hao,J., Honchak,B.M., Karbach,L.E., Kurdoglu,A., Lahiri,S.,Mastrian,S.D., Miyashita,H., Page,L., Ramakrishna,P., Satoh,S., Sattley,W.M., Shimada,Y., Taylor,H.L., Tomo,T., Tsuchiya,T., Wang,Z.T., Raymond,J., Mimuro,M., Blankenship,R.E. and Touchman,J.W. (2008) Proc. Natl. Acad. Sci. U.S.A.
2] ISfinder annotation (2009)