ISAcma14

  • Family IS630
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_009925 ND Acaryochloris marina
Acaryochloris marina MBIC11017
Acaryochloris marina plasmid pREB5
Acaryochloris marina plasmid pREB2
DNA section
IS Length : 1138 bp

Ends


IR Length : 15/28

IRL : CTGCGGACGACTTGTGACTTAACATTGTGGTAGAGAGATTTGGCACACTG
IRR : CTCTAGGCGGCTAAGATATTAACTTTCTTAAAAGACTGAAACTTGAGTGA

Insertion site


Left flankDirect repeatRight flankDR Length
ATTCACTACATAAAGCTGGAAAACGCTATAAGTTTCCTATTTCAAATTAATCTTGT4
GCTCTCTATATCAGGCATTGTACTTTTATATCAATTATGCATGTACTCATTAGGTT4
TCTAATTACTGGGGCATTAATCTAGATATAGTATTTGGGGGCCAAGACCAAGTTGC4
TTCTTGGATGCCTCAATGACTTGCGTTATAGCCCTCTTTTATCACTTTAGACAGAT4
AAAATGCGTACTATGCGATTGGCTGTTATATAGCTGCCATCTCTACAGTGATGCGA4
GATAAAAATTACTGGAATTTCACGGGTATAACGGGATCTGCCTTTAGATGTTCACA4
AAGATATATCCCAAGTAATAAAAAGTTATACCCCTCTTTTATCACTTTAGACAGAG4

DNA sequence

CTGCGGACGACTTGTGACTTAACATTGTGGTAGAGAGATTTGGCACACTGGGAGTATCCCCGCACCTATCCTCCACTCCCATGATTAAAATTGATTTCAC
CGCAGAGGAAATCCAACAACTCAATTACGAACGCTATCATCACCCTCACCCACGGGTGCAGCGACGGATGGAAGTGCTCTACTTGAAGAGTCAAGGTCTT
TCCCATACCCAGATCTGCCAGCTTTGTCAGATTAGTCGCCCCACTTTAGCCAAAACTCTTCGTCAGTATCAACAAGGAGGGATTGAAGGGCTGAAGACCC
TTGAATATAAAGGTCAACCGAGTCTACTCAACGCTCATAGCGATAGTGTGAGAGCTTACTTTGAGCAACATCCTCCCCGCACCAGTGCAGAAGCTCAAGC
GGTCATTGAGCAATTAACAGGCATCAAACGCAGTCCCACCCAAATCAAAGCCTTCCTCAAACGCATCGGTTGTCGTTATCGAAAAGTGGGGTATGTGCCG
GGAAAGTCGAGTCTGCCCGAGAAAATTGAGGAGCAAGAGCAGTTTCGACACACTCGCCTTGAACCCTTATTAGAGGAAGCTCAACGCCAAGAGCGTCTTG
TCTTTTTTGTGGATGCTGCTCACTTTGTTCACCGTGCTTATCTAGGGTTTGTCTGGTGCATCACTCGGATCTTCATCCCTTCTCCTTCTGGTCGCCAACG
TTTCAATGTCCTGGGCGCGCTCGACGCTATCACCAAAGACCTAGTCAGCGTTACCAACCACACCTATATCAATTCTCACAGTATGTGCCTGTTACTGGCC
AAGCTGGCGCTACTTGACCCAGTCATACCCATCAGCGTGATTTTAGACAATGCGCGATACCAGAAATGTCAGCTTGTAACGGACTTTGCCGAGATCGTCG
ATATTGAGCTGGTCTATCTGCCCTCCTATTCACCCCATTTGAATTTGATTGAGCGACTCTGGCGTTTTGTACGCAAGGAATGTCTCTATTCAAAGTATTA
TGCTGATTTTCCCGCCTTCAAAGGGGCCATTCAACAGTGCATCGACCAGTGTAATGGAGAGCATAAGGCGAAACTCACCACCTTACTATCACTCAAGTTT
CAGTCTTTTAAGAAAGTTAATATCTTAGCCGCCTAGAG
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1110 bp369 aa271136+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MVERFGTLGVSPHLSSTPMIKIDFTAEEIQQLNYERYHHPHPRVQRRMEVLYLKSQGLSHTQICQLCQISRPTLAKTLRQYQQGGIEGLKTLEYKGQPSL
LNAHSDSVRAYFEQHPPRTSAEAQAVIEQLTGIKRSPTQIKAFLKRIGCRYRKVGYVPGKSSLPEKIEEQEQFRHTRLEPLLEEAQRQERLVFFVDAAHF
VHRAYLGFVWCITRIFIPSPSGRQRFNVLGALDAITKDLVSVTNHTYINSHSMCLLLAKLALLDPVIPISVILDNARYQKCQLVTDFAEIVDIELVYLPS
YSPHLNLIERLWRFVRKECLYSKYYADFPAFKGAIQQCIDQCNGEHKAKLTTLLSLKFQSFKKVNILAA

 

Blast result :
Comments
ISAcma14 is 47% aa similar to ISPlu3.
References
1] Swingley,W.D., Chen,M., Cheung,P.C., Conrad,A.L., Dejesa,L.C.,Hao,J., Honchak,B.M., Karbach,L.E., Kurdoglu,A., Lahiri,S.,Mastrian,S.D., Miyashita,H., Page,L., Ramakrishna,P., Satoh,S., Sattley,W.M., Shimada,Y., Taylor,H.L., Tomo,T., Tsuchiya,T., Wang,Z.T., Raymond,J., Mimuro,M., Blankenship,R.E. and Touchman,J.W. (2008) Proc. Natl. Acad. Sci. U.S.A.
2] ISfinder annotation (2009)