ISAcma16

  • Family IS4
  • Group IS4
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_009925 ND Acaryochloris marina
Acaryochloris marina MBIC11017
DNA section
IS Length : 1483 bp

Ends


IR Length : 18

IRL : CAATGGCACTGACTTAAGAAGTCAGGAAAAAGCTACAAGTCTTGATAGAT
IRR : CAATGGCACTGACTTAAGCTCAATCAAACAGCTAGCTTAGCTTTGAGAGC

Insertion site


Left flankDirect repeatRight flankDR Length
TCAGATCAATCACATCCCCCACCCCCAGATAGCGCACCTGGCTCGCCCTC10
AGCAATGAGGTATTGACTATCCCTTGGAATATCTTTCTCCTTGACAAC12
CTACCATTGGGTCGATGGCTAGCTATAGTTTATTCGCTCTTTCTAGCCAG10
AAATATTGAAAGGGAGAAAGCCCCATAGATTGCTTGAACAGACGGCTAAA10
AGAAGGCGAATCTATCGCTATTGCATCGTTCAATCTCTCAACGCTGAAT11
GTCGGTATTTCCAGATACGTCCCTATGGGTTGCCATCCATAGGGAATAC11
CCCAATGTTCAAGAGAGCTATCCCAACAGTGGTTGTTCAAGAATGAACT11
CTCAATTCCCTGCCTGATCAGTCTATAGATGCTTTGAAAATTCAGTAATT10
GTTTGCTGAGTCGTTTAAGCAGTCCTGATTCGCCCAGAATATCTTCCGGT10
ATCACCGCCTTGGGATAAACTATTAAAGTTGGCTTGACCTGAATCGGGCA10
GGGAAATCCTTTCCCAAGTTATTTATGGATTCCTGCAGTTTTTGCCCAAA10
AGTTCATTCTTGAACAACCACTGTTGGGATAGCTCTCTTGAACATTGGG11
AGGAAACGCTGCATCTGTCATCTAACGGATAGGTCTCAGGGGTGCGAGA11

DNA sequence

CAATGGCACTGACTTAAGAAGTCAGGAAAAAGCTACAAGTCTTGATAGATATAGGATCAAGACTTGCACGCCATAAACTTCTGCCTTTCACTATGTCTGA
GTCAGCCGAAATTCTCAAGCAGCAATTTTCCCAAAGCCTGGGTTTACCGTGGACAGATATCCTTCCAGCCTCAAGGTTAGAGGAACTCCTGAAAGAAGAA
GCATTCTCCTATCGCAACCGGATATATAGCCCCATCGTTACGCTGTGGGCCATGCTTTACCAAGTGCTATCAGCCGATAAAAGCCTTCGTAACACAGTCA
AATGCATCACGACTTGGCTCACAGCGGCTGGCATCCAACCGCCCTCATCTGATACCGGAGCCTACAGCAAAGCCAGAAGTCGCTTTCCAGAGTCACTGTT
GCAACGTTTAATTCCCGAAAGCGCTGAGTGCTTAGCGCAACCCCTCTCCCCAGAGCACCTCTGGTGTGGTCGGCCCGTCAAGGTCTACGACGGAACCACC
GTGTTGATGGCTGATAGTGCGGCCAACCAAGCATCATATCCACAACATGGCAATCAAACAGCAGGCTGTGGTTTTCCCATCGCTCGCTTGGTAGTGTTCT
TCTGTTTGGTTACCGGTGCAGTGGCGTCAGCTTGTATTGCCTCCTGGGACACCAGTGAAATTGTCATGAGTCGTTTGCTCTATCAAGACCTTGAGGTCGG
TGATGTGGTCATGGCGGACCAAGCTTATGGCAGCTATGTTGATCTAGCCATCATTCAACAACACAGGGCTGATGGAGTGTTGCGTAAACATCATGCTCGC
AAGACTGATTTCCGCAAAGGCAACAAGCATGGCATTGGTGATCATCAGGTGACATGGCATAAGCCAGCCCAACGGCCTGAGCACATGAGTGAGCAAGATT
TTGCCCTGATTCCTCAAACATTGGTCGTTAGAGAGGTGTGTTTGCGCTTATCCCTTAAGGGCTTTCGCGACCAGCACATTATTGTGGTGACGACGCTGCT
GGATGCTCAACGCTACAGCGCTGGGCAACTGACTCGCTTGTATGGCTGGCGTTGGCCAGTGGCGGAAGTCAATCTGCGCCATCTCAAAACCACCTTAAAA
ATGGAGATGCTCAGTGCCAAAACTCCGGATATGGTGCGCAAGGACATTTGGGTACATTTGTTGGGCTATAACCTACTCAGAAGTCTCATGGAACTTGCGG
CACCGCTAGCAGATAATGCTAGAACTCAACTGTCTGTGCAAGGAGCACGACAACACTTCAATCAGATGCTTGCTTTGTTGGCGACAGCCAACCGTGCGAC
CAGAAAGCGGTTGTTTACTCATCTACTTGAGACCATGGCAGCCGATCTATTACCCTCTCGACCGAATCGGCACGAACCGAGAGTCGTCAAACGCAGACCC
AAATCTTTCCCGCGAATGCGACAACCTCGCTCTGCTCTCAAAGCTAAGCTAGCTGTTTGATTGAGCTTAAGTCAGTGCCATTG
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1419 bp472 aa421460+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MIDIGSRLARHKLLPFTMSESAEILKQQFSQSLGLPWTDILPASRLEELLKEEAFSYRNRIYSPIVTLWAMLYQVLSADKSLRNTVKCITTWLTAAGIQP
PSSDTGAYSKARSRFPESLLQRLIPESAECLAQPLSPEHLWCGRPVKVYDGTTVLMADSAANQASYPQHGNQTAGCGFPIARLVVFFCLVTGAVASACIA
SWDTSEIVMSRLLYQDLEVGDVVMADQAYGSYVDLAIIQQHRADGVLRKHHARKTDFRKGNKHGIGDHQVTWHKPAQRPEHMSEQDFALIPQTLVVREVC
LRLSLKGFRDQHIIVVTTLLDAQRYSAGQLTRLYGWRWPVAEVNLRHLKTTLKMEMLSAKTPDMVRKDIWVHLLGYNLLRSLMELAAPLADNARTQLSVQ
GARQHFNQMLALLATANRATRKRLFTHLLETMAADLLPSRPNRHEPRVVKRRPKSFPRMRQPRSALKAKLAV

 

Blast result :
Comments
ISAcma15 is 57% aa similar to ISArch10.
References
1] Swingley,W.D., Chen,M., Cheung,P.C., Conrad,A.L., Dejesa,L.C.,Hao,J., Honchak,B.M., Karbach,L.E., Kurdoglu,A., Lahiri,S.,Mastrian,S.D., Miyashita,H., Page,L., Ramakrishna,P., Satoh,S., Sattley,W.M., Shimada,Y., Taylor,H.L., Tomo,T., Tsuchiya,T., Wang,Z.T., Raymond,J., Mimuro,M., Blankenship,R.E. and Touchman,J.W. (2008) Proc. Natl. Acad. Sci. U.S.A.
2] ISfinder annotation (2009)