ISAcma18

  • Family IS4
  • Group IS50
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_009925 ND Acaryochloris marina
Acaryochloris marina MBIC11017
DNA section
IS Length : 1530 bp

Ends


IR Length : 16/19

IRL : CTGACTTTTCACGGTAAATTCTAGAGCGCTTTCCCCATTGCGATTGTAGG
IRR : CTGACTTTTCCCGCTAAGTCTCCTGGGTCGCTAACTGCCAGCCCTCGACT

Insertion site


Left flankDirect repeatRight flankDR Length
ACACCCTTACTCCCCAATAAATTCATTGACCTAGTTTTTGACAAGAAAGG10
CACCTAACCAAAAACGGCAGATGGTGAGACCTCCACAGAAGCGCCATCATTCCAGTACAA0
TCTCAAAATTTCCTCACACTCCTCCACAGAGGTGAGACAGTTGCAGCAGGACTATTCCAT0
ACTTTTTCAACCAAGCGACATCACTTAAAGTTGGTAGTGAAATACACCCAA9
ATACCTGCATGGATTGGTAACAGTTCCTACACCCAAAAAGTAGGGCTTGATC8

DNA sequence

CTGACTTTTCACGGTAAATTCTAGAGCGCTTTCCCCATTGCGATTGTAGGGAATTGTTCTCCATTCAGCTACGATGACCAAATCCTGAGTTTGGAGATGA
TTAGGATGCAGACATGGTGGAAGAAGAATTTTGCTGAGTGTCAATTCAACGATGACCGAAGGTCGGTCGTAGACCATCAGCGCTTGACCCATCGAGCTCT
CAAAATTGGTCAAGCGATTAGTGCAAACTTCGGCCAAGCCCTGTTGAGCATTTTCAAGACTGCAACAGATCTCAAACGCTCTTATGAGTTTTCGCTAATG
TGAAAACCAACTTCTCTCAAATCATTACTCCTCATTGCCATGCAACTGCCCATGCCATCACCGAGGAAGAGATTACCTTGTGCGTGGGTGATACGAGCTT
TTTAGACTACGGAGGCATCAAGAAGAAGCGTCAAGGCTATGGTCCTCAAGGCAATGGGGGGAATGGCTTATTGCTCCACAGTGCCTTAGCTGTTGACCCC
GAGCAAGGCCAACCATTGGGGTTGCTATGGCAAAAGGTATGGAATCGTCAGCATCGACCCCAGCCTCCGAAAAAGGAAACCCCTCGACAAAAGAAGAAGC
GTCAGGCAAAGGCGCGACAAACTTCTCGGAACCGACCCTTTGAACAAAAGGCATCCTATCGGTGGGTCGAAGCGATGCAAGCCGTAGAAAAGATAGTGAG
TCCTTCTACTCGTTCCATTCATGTGTTTGACCGAGAAGGAGATATTGCCGAAGTGTTTGAGCGGCTCAATCACCTCAACAACACAGGGGTTGTGGTTAGA
GCCTCACATAATCGTCGGCTTGAGCAAGACCCCAATCGACTGTGGAAAAAACTGGAAGCCCAAAGTATTCAATTCGAGTATGAAATTGACTTACCCAAAA
CGAAAGACCGGAGTGCACGTACAGCCAAGCTAGTGGTGCGCTGTTGTCTCGTCCAACTGCAGCGACCTACCCGTCTAGCTGATAGTCATCCCCTCCAGGT
ATACGCCGTCTATGCCACAGAAGTAGATCCCCCAGAGGATGAAGACCCAGTGTCATGGATGTTGCTCACGAGTGAAGCAGTCACCACTATGGAAATGGCT
CAGACCATTTTGCGCTGGTACACGTACAGGTGGAGAGTGGAGGAATATCACAAAATTCTCAAATCGGGGACTCAGGTGGAGCGGTATCGTTTGGCTGCTG
AGGGGATGAAAACCTTATTAGGATTCCTGTGTGTCACTGCTGTAGAACTGTTACGCTGGACCTACTTAGAACGCACATCCCCTCAACGGCCTGCTCAAGA
TGTCGTCACACCTTTACAGATTAAAGTTCTCAGAGCGAAGACATCGAAGGTTCCCAAAAACCTCACGGTTGCATGGGCAGTGAAAGCAGTTGCACGACTT
GGAGGATATCTGGAGCATCGTCGCAACACACCGATGGGGATTCAAGTGCTTTGGAAAGGATGGGCTAAGTTAAATCTCCTAGTCGAGGGCTGGCAGTTAG
CGACCCAGGAGACTTAGCGGGAAAAGTCAG
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1218 bp405 aa3001517+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MKTNFSQIITPHCHATAHAITEEEITLCVGDTSFLDYGGIKKKRQGYGPQGNGGNGLLLHSALAVDPEQGQPLGLLWQKVWNRQHRPQPPKKETPRQKKK
RQAKARQTSRNRPFEQKASYRWVEAMQAVEKIVSPSTRSIHVFDREGDIAEVFERLNHLNNTGVVVRASHNRRLEQDPNRLWKKLEAQSIQFEYEIDLPK
TKDRSARTAKLVVRCCLVQLQRPTRLADSHPLQVYAVYATEVDPPEDEDPVSWMLLTSEAVTTMEMAQTILRWYTYRWRVEEYHKILKSGTQVERYRLAA
EGMKTLLGFLCVTAVELLRWTYLERTSPQRPAQDVVTPLQIKVLRAKTSKVPKNLTVAWAVKAVARLGGYLEHRRNTPMGIQVLWKGWAKLNLLVEGWQL
ATQET

 

Blast result :
Comments
ISAcma18 is 50% aa similar to ISGvi2.
References
1] Swingley,W.D., Chen,M., Cheung,P.C., Conrad,A.L., Dejesa,L.C.,Hao,J., Honchak,B.M., Karbach,L.E., Kurdoglu,A., Lahiri,S.,Mastrian,S.D., Miyashita,H., Page,L., Ramakrishna,P., Satoh,S., Sattley,W.M., Shimada,Y., Taylor,H.L., Tomo,T., Tsuchiya,T., Wang,Z.T., Raymond,J., Mimuro,M., Blankenship,R.E. and Touchman,J.W. (2008) Proc. Natl. Acad. Sci. U.S.A.
2] ISfinder annotation (2009)