ISfinder Mirror Site

ISAcma15

Family IS630
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_009925	ND	Acaryochloris marina	Acaryochloris marina MBIC11017 Acaryochloris marina plasmid pREB5

DNA section

IS Length : 1083 bp

Ends

IR Length : 22/29

IRL : AGTAGATAGCCAGAAATATTTACATCATAATAAGTGGTTTCAGTTCAGTG
IRR : AGTACCTATCCAAAATCAATTACATAATCTCTTCCGAAGCCCTTAAGGAT

Insertion site

Left flank	Direct repeat	Right flank	DR Length
GGCTGTGGTTTTCCCATCGCTCGCTTGG	TA	GTGTTCTTCTGTTTGGTTACCGGTGCAG	2
CTCGTCCCATTGCACCCGTCCCGCCAAT	TA	CCAAAGCTGTCTTTATCTCTGACATATA	2
TTTAATAACAGCTGATTCAGAGTTGTAG	TA	TCAATTTCTAGCTGACGACTCAGTTGCT	2

DNA sequence

AGTAGATAGCCAGAAATATTTACATCATAATAAGTGGTTTCAGTTCAGTGGATAGCGAATAGCTTATAGCCCAATGAAACAACGTTTTATTCAAGGATTG
AGCCCAGAGACCATCCATTTACTCAGCCGAATTCATCGCCATAGTCATCATCACCAAGTCCGTCAACGCGCCCATTGCATTCTCTTGAGTTTTGAAGGGT
TTAATGTCACCGAGTTAATGAGCATCTTTGCCGTCACCCGAAAAACGGTTTACACCTGGTTAGATGCTTGGGACAACCACTGCTTAGTGGGTCTGTATGA
TCAGCCGGGCCGTGGCCGTAAACCTAAGTTGAGAGACGTGCAGAAGGAGCAAATCCGAGAGTGGGCAAAAATGACTCCGCATAACCTCAATGTTGTGTTG
GCAAAGATCAAGGAAGCCTGGAACATAGAGGTGAGCAAAACCACCCTCAAGCGCATCCTCAAATCATGTTCCATGAGCTGGCGTCGCCTCAGGCGAGTCG
TTGCGGGCCAGCCAGACCCAGTGGAGTATGCAACCAAACGTCATCAGTTAGAGGTCTTGAAGCGTCAGGAGGAAAAAGGTGAGCTGGACCTGAGATATCT
CGATGAAAGCGGGTTTTGCTTAGTGCCGTATGTCCCCTATGCTTGGCAGGAGAAAGGGGAAACACTAGGACTTCCCAGTCAACGCAGCGGTCGATTCAAC
GTGTTGGGGTTGATGAATCGGCACAATGACCTCACTTCCTATGTGTTTGACAAGAGTATTACCAGTGCGGTGGTGGTGGCCTGTATTGATGATTTTAGTC
AGACCTGTGACCAACACACGGTCGTGGTGATGGATCAAGCCAGCATCCATAAAAACACTGAAATTGAAGAAAACATTGAGGACTGGAAAGCCAAGAATGT
CGAAATTTTCTGGCTACCTACCTATTCACCGCATCTGAATTTGATTGAGATTTTCTGGCGTTTTATGAAATATGAGTGGCTTGAATTTGATGCCTACAAG
TGTTTAGGCAGCCTGAGTCTGTATATCGACAAAATCCTTAAGGGCTTCGGAAGAGATTATGTAATTGATTTTGGATAGGTACT

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
1005 bp	334 aa	74	1078	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MKQRFIQGLSPETIHLLSRIHRHSHHHQVRQRAHCILLSFEGFNVTELMSIFAVTRKTVYTWLDAWDNHCLVGLYDQPGRGRKPKLRDVQKEQIREWAKM
TPHNLNVVLAKIKEAWNIEVSKTTLKRILKSCSMSWRRLRRVVAGQPDPVEYATKRHQLEVLKRQEEKGELDLRYLDESGFCLVPYVPYAWQEKGETLGL
PSQRSGRFNVLGLMNRHNDLTSYVFDKSITSAVVVACIDDFSQTCDQHTVVVMDQASIHKNTEIEENIEDWKAKNVEIFWLPTYSPHLNLIEIFWRFMKY
EWLEFDAYKCLGSLSLYIDKILKGFGRDYVIDFG

Blast result :

Comments

ISAcma15 is 46% aa similar to ISTth6.

References

1] Swingley,W.D., Chen,M., Cheung,P.C., Conrad,A.L., Dejesa,L.C.,Hao,J., Honchak,B.M., Karbach,L.E., Kurdoglu,A., Lahiri,S.,Mastrian,S.D., Miyashita,H., Page,L., Ramakrishna,P., Satoh,S., Sattley,W.M., Shimada,Y., Taylor,H.L., Tomo,T., Tsuchiya,T., Wang,Z.T., Raymond,J., Mimuro,M., Blankenship,R.E. and Touchman,J.W. (2008) Proc. Natl. Acad. Sci. U.S.A.
2] ISfinder annotation (2009)