ISAcma13
- Family IS3
- Group IS150
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_009925 | ND | Acaryochloris marina | Acaryochloris marina MBIC11017 |
DNA section
IS Length : 1263 bp
Ends
IR Length : 22/25
IRL : TGAAGTGACCCCCAAGATTCGGACAAGTCCTTAAGCATCGTAATCTTGTA
IRR : TGAAGTGAACCCCTATATTCGGACAGGGCAATAGATGAATTAAGCGCTAT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TAAAACTGAGGTCTGATCAAGACTGGC | ATC | CTCATGAAGGCGAGCAGTGCGATCGCT | 3 |
| CTTTCAGGAAGTAATTCTAGGTCCGAG | GTG | GTCCAGCGAACACGCTCTTCTATTGCA | 3 |
| TTGTTGAGTGATAACCGTTAAGTTGAC | TCG | CCTAACACCAGCAGTCATCGTTGCCCG | 3 |
| GTAAAGCGCAGGGCTACAGTACTAGTTAAG | TCGCCATTCCTAAAGACCCATGTATTTGCC | 0 |
DNA sequence
TGAAGTGACCCCCAAGATTCGGACAAGTCCTTAAGCATCGTAATCTTGTACCGAATGAGGGAAATTAATGTCATCAAAACGTCGTCAATATAGTGCTGAG
TTCAAAGCTCAAGTGGTCTTACAATTGTTAAGCGGTGAAAAAACGAGCAGTGCATTGTGTCGCATCCATACCCTGCACTCTAGCCTCCTCAACCGCTGGC
GCAAGGAATTTCTAGCGCACGCCAGTAGCATTTTTGAGAGTCAGAACAACGACGACAACGCTGAAGCGAAAATTGCTCAGTTAGAGCGTCTAGTCGGCCA
ATTGACGCTGCAATTAGAGATCGCAAAAAAAGCCTCAATGTCCTTACACCTAACCAAAAACGGCAGATGGTGAGACAGTTGCAGCAGGACTATTCCATTC
GCCAAATTTGTCAGGTACTCAATTACCCACGCTCTCAAGTGTATTACCATGCCAGAGGCCAACCGGATGAGAGCGAACTAAAAGCGGCCATTGCTGGAGT
TGCTGGAGCCTATCCGACTTATGGCTATCGTCGAATCACCGCCCAACTGCAGCGTCAGGGGTATTGCGTTAACCATAAACGCGTGGCTCGACTAATGCGA
CAGATAGGGATTATGGCCAAGACCAAAGTCAAACGCAAACGCACCACCAATAGCGAACATTCGTTCCCTCGCTATGGCAATCGAGTGTTGAATCTCTCCA
TCGACCATCCTGAGCAGGTGTGGGTGGCGGACATCACGTACATTCGCTTACAGCAGGAGTTTGTCTATTTGGCCGTCGTGATGGATGTCTTTACTCGCGC
CATTCGTGGCTGGCATCTGTCCCGCCATATCGACCAGCAACTAACGTTGAGGGCGTTGAACAAGGCGTTGGAGCGGGCAACGCCTGAGATTCACCATTCT
GACCAAGGGGTTCAGTATGCCGCTGCAGCCTATATGCAATTGCTCCAACAACACCAAGTGCAAATCAGTATGGCCGAGGTGGGACAGGCTTGGCAAAATG
GCTATGCTGAGCGATTGATGAGAACGATAAAAGAAGAAGAGGTCGATTTATCAGACTATCGAAATTTTACTGAAGCGTATGAGCACATTGAGCAGTTTCT
AGAAGATGTGTATATGCATAAGCGGATTCATTCATCACTGGGGTATTTGACACCCTGTGAATATGAGCAGCAGTGGAGACAACAGAACAATCACTATTGT
ATGAATAAGGAGGATAGCGCTTAATTCATCTATTGCCCTGTCCGAATATAGGGGTTCACTTCA
TTCAAAGCTCAAGTGGTCTTACAATTGTTAAGCGGTGAAAAAACGAGCAGTGCATTGTGTCGCATCCATACCCTGCACTCTAGCCTCCTCAACCGCTGGC
GCAAGGAATTTCTAGCGCACGCCAGTAGCATTTTTGAGAGTCAGAACAACGACGACAACGCTGAAGCGAAAATTGCTCAGTTAGAGCGTCTAGTCGGCCA
ATTGACGCTGCAATTAGAGATCGCAAAAAAAGCCTCAATGTCCTTACACCTAACCAAAAACGGCAGATGGTGAGACAGTTGCAGCAGGACTATTCCATTC
GCCAAATTTGTCAGGTACTCAATTACCCACGCTCTCAAGTGTATTACCATGCCAGAGGCCAACCGGATGAGAGCGAACTAAAAGCGGCCATTGCTGGAGT
TGCTGGAGCCTATCCGACTTATGGCTATCGTCGAATCACCGCCCAACTGCAGCGTCAGGGGTATTGCGTTAACCATAAACGCGTGGCTCGACTAATGCGA
CAGATAGGGATTATGGCCAAGACCAAAGTCAAACGCAAACGCACCACCAATAGCGAACATTCGTTCCCTCGCTATGGCAATCGAGTGTTGAATCTCTCCA
TCGACCATCCTGAGCAGGTGTGGGTGGCGGACATCACGTACATTCGCTTACAGCAGGAGTTTGTCTATTTGGCCGTCGTGATGGATGTCTTTACTCGCGC
CATTCGTGGCTGGCATCTGTCCCGCCATATCGACCAGCAACTAACGTTGAGGGCGTTGAACAAGGCGTTGGAGCGGGCAACGCCTGAGATTCACCATTCT
GACCAAGGGGTTCAGTATGCCGCTGCAGCCTATATGCAATTGCTCCAACAACACCAAGTGCAAATCAGTATGGCCGAGGTGGGACAGGCTTGGCAAAATG
GCTATGCTGAGCGATTGATGAGAACGATAAAAGAAGAAGAGGTCGATTTATCAGACTATCGAAATTTTACTGAAGCGTATGAGCACATTGAGCAGTTTCT
AGAAGATGTGTATATGCATAAGCGGATTCATTCATCACTGGGGTATTTGACACCCTGTGAATATGAGCAGCAGTGGAGACAACAGAACAATCACTATTGT
ATGAATAAGGAGGATAGCGCTTAATTCATCTATTGCCCTGTCCGAATATAGGGGTTCACTTCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 306 bp | 101 aa | 68 | 373 | + | No |
Description : First part of the transposase
ORF sequence :
MSSKRRQYSAEFKAQVVLQLLSGEKTSSALCRIHTLHSSLLNRWRKEFLAHASSIFESQNNDDNAEAKIAQLERLVGQLTLQLEIAKKASMSLHLTKNGR
W
W
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 960 bp | 319 aa | 265 | 1224 | + | No |
Description : Second part of the transposase
ORF sequence :
SENCSVRASSRPIDAAIRDRKKSLNVLTPNQKRQMVRQLQQDYSIRQICQVLNYPRSQVYYHARGQPDESELKAAIAGVAGAYPTYGYRRITAQLQRQGY
CVNHKRVARLMRQIGIMAKTKVKRKRTTNSEHSFPRYGNRVLNLSIDHPEQVWVADITYIRLQQEFVYLAVVMDVFTRAIRGWHLSRHIDQQLTLRALNK
ALERATPEIHHSDQGVQYAAAAYMQLLQQHQVQISMAEVGQAWQNGYAERLMRTIKEEEVDLSDYRNFTEAYEHIEQFLEDVYMHKRIHSSLGYLTPCEY
EQQWRQQNNHYCMNKEDSA
CVNHKRVARLMRQIGIMAKTKVKRKRTTNSEHSFPRYGNRVLNLSIDHPEQVWVADITYIRLQQEFVYLAVVMDVFTRAIRGWHLSRHIDQQLTLRALNK
ALERATPEIHHSDQGVQYAAAAYMQLLQQHQVQISMAEVGQAWQNGYAERLMRTIKEEEVDLSDYRNFTEAYEHIEQFLEDVYMHKRIHSSLGYLTPCEY
EQQWRQQNNHYCMNKEDSA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1157 bp | 386 aa | 68 | 1224 | + | Yes |
Chemistry : DDE
ORF sequence :
MSSKRRQYSAEFKAQVVLQLLSGEKTSSALCRIHTLHSSLLNRWRKEFLAHASSIFESQNNDDNAEAKIAQLERLVGQLTLQLEIAKKSLNVLTPNQKRQ
MVRQLQQDYSIRQICQVLNYPRSQVYYHARGQPDESELKAAIAGVAGAYPTYGYRRITAQLQRQGYCVNHKRVARLMRQIGIMAKTKVKRKRTTNSEHSF
PRYGNRVLNLSIDHPEQVWVADITYIRLQQEFVYLAVVMDVFTRAIRGWHLSRHIDQQLTLRALNKALERATPEIHHSDQGVQYAAAAYMQLLQQHQVQI
SMAEVGQAWQNGYAERLMRTIKEEEVDLSDYRNFTEAYEHIEQFLEDVYMHKRIHSSLGYLTPCEYEQQWRQQNNHYCMNKEDSA
MVRQLQQDYSIRQICQVLNYPRSQVYYHARGQPDESELKAAIAGVAGAYPTYGYRRITAQLQRQGYCVNHKRVARLMRQIGIMAKTKVKRKRTTNSEHSF
PRYGNRVLNLSIDHPEQVWVADITYIRLQQEFVYLAVVMDVFTRAIRGWHLSRHIDQQLTLRALNKALERATPEIHHSDQGVQYAAAAYMQLLQQHQVQI
SMAEVGQAWQNGYAERLMRTIKEEEVDLSDYRNFTEAYEHIEQFLEDVYMHKRIHSSLGYLTPCEYEQQWRQQNNHYCMNKEDSA
Blast result :
Comments
ISAcma13 is 60% aa similar to ISTth1.
References
1] Swingley,W.D., Chen,M., Cheung,P.C., Conrad,A.L., Dejesa,L.C.,Hao,J., Honchak,B.M., Karbach,L.E., Kurdoglu,A., Lahiri,S.,Mastrian,S.D., Miyashita,H., Page,L., Ramakrishna,P., Satoh,S., Sattley,W.M., Shimada,Y., Taylor,H.L., Tomo,T., Tsuchiya,T., Wang,Z.T., Raymond,J., Mimuro,M., Blankenship,R.E. and Touchman,J.W. (2008) Proc. Natl. Acad. Sci. U.S.A.
2] ISfinder annotation (2009)
2] ISfinder annotation (2009)