ISAcma11

  • Family IS4
  • Group ISPepr1
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_009925 ND Acaryochloris marina
Acaryochloris marina MBIC11017
DNA section
IS Length : 1347 bp

Ends


IR Length : 19

IRL : CAATACTCCGGACAGTTTTGTAGTAGCTGGTGAGAAGAAAAGCATCCACC
IRR : CAATACTCCGGACAGTTTTAATGGTCGATAGCTCCGTACTTAAGGAGTTT

Insertion site


Left flankDirect repeatRight flankDR Length
CTTATCTATTTTGGCCTTAATTCCTACTAGGATTATTCAAAGTCAAAACTGTTA6

DNA sequence

CAATACTCCGGACAGTTTTGTAGTAGCTGGTGAGAAGAAAAGCATCCACCGTGTAGAGTGGAGTTATCCTTTAATAGACTGCACCGATGGATACCCTTAA
AGCAATTCTAATGGGTCTGCTGCAATCAGCAGGCCATTTCGCAAACCCCAACAGACGTTTTTGCTGAGCCTGATGACGGCGATTTTCGTCGTTTGTGGCA
AAGTCAACTAAACAAACCTCAGCCGTTATAGCCATTTATGCGAGAAAACCTATCGGCGGCAGTTTAATGCGGGTCTGAGTCTGGAGCCTCTTAACAGCGC
TCTTATTGAAGAAGTCGCAGCTCCAGAGAGTGAGCAAATTGCAGCCGTCGACTGTACCTTTGTGGAGAAAAGTGGTCACTACACCCATGGAGTAGATTGG
TTCTTCAACGGTAAAACCCAACGTGCAGAACGGGGGATTGAGTGGTCTGTCATTGCTATTATTGACCTGGCTCAACACACCGGATATACCCTCTCAGCTC
AACAGACCGAAGCTGGATTACAAGCCAAGCCCCCTGAGCCCTCCAGACTAGCCAAGGACAGCCCATCTCCCACTAGCCGAGTTGATTTTTACGTGGGGCA
TCTCGTTGAGTGTGACTCCTATTTGCCTGAGCGTGTGTGCTATATCGTTGCTGATGGATTTTACAGTAAACGAAAGTGGATCAATGCGGTTGTGCAAATC
AACCGACATGCCATTGGTAAGCTCCGCTGCGATGCCAATCTCAAGTTTCTCTATCGCGGTCCGCAAAAGCGCCAAGGGCGTCGTCGTCGATATGCAGCCA
AGGTCGAATTAGCAGACCCGAGCAACTTGACGTTTGTCTGTGAACTTGAATCGGGAGACCAGTTGTATATGGCTGTGGTGTGGTCTGTTTCACTCAAGCG
GGCGATTCGAATGGCCTATGTGTGCGGACATAAAGACGGCAAACACAGCTATGCCGTTTTGTTCTCAACGGACTTAGACATAGATCCGTATGATATTTAT
CGCTATTACAGTGCCCGGTTCCAAATTGAGTTTATTTTTCGGGATGCTCGACTGTTTACAGGGCTGACGCATTGTCAAGTGCGTAATGCCAATGCCCTAG
ATTCACATGTCAATGCCAGCCTATTGGCGCTCAACTTGGCTAAGGCAGCCTGGAGACGTGACTGTCAAAAAGCGGAGCCCAGTCGTTTCTCGATAGCATC
GTTCAAACGTTGGGCGCTCAATGAGCATCTGCTCGAGTTATTTATTGACATGTTTGAGTTGGACCCAACTTTGATTAAATCCCACATCAACTACCAGAAA
CTCCTTAAGTACGGAGCTATCGACCATTAAAACTGTCCGGAGTATTG
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
969 bp322 aa3621330+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MEKSGHYTHGVDWFFNGKTQRAERGIEWSVIAIIDLAQHTGYTLSAQQTEAGLQAKPPEPSRLAKDSPSPTSRVDFYVGHLVECDSYLPERVCYIVADGF
YSKRKWINAVVQINRHAIGKLRCDANLKFLYRGPQKRQGRRRRYAAKVELADPSNLTFVCELESGDQLYMAVVWSVSLKRAIRMAYVCGHKDGKHSYAVL
FSTDLDIDPYDIYRYYSARFQIEFIFRDARLFTGLTHCQVRNANALDSHVNASLLALNLAKAAWRRDCQKAEPSRFSIASFKRWALNEHLLELFIDMFEL
DPTLIKSHINYQKLLKYGAIDH

 

Blast result :
Comments
Only one copy in the genome.
ISAcma11 is 49% aa similar to ISDra7.
The transposase is probably shorter than expected in N terminal part.
References
1] Swingley,W.D., Chen,M., Cheung,P.C., Conrad,A.L., Dejesa,L.C.,Hao,J., Honchak,B.M., Karbach,L.E., Kurdoglu,A., Lahiri,S.,Mastrian,S.D., Miyashita,H., Page,L., Ramakrishna,P., Satoh,S., Sattley,W.M., Shimada,Y., Taylor,H.L., Tomo,T., Tsuchiya,T., Wang,Z.T., Raymond,J., Mimuro,M., Blankenship,R.E. and Touchman,J.W. (2008) Proc. Natl. Acad. Sci. U.S.A.
2] ISfinder annotation (2009)