ISAzo41

  • Family IS5
  • Group IS5
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_006823 ND Azoarcus sp.
Aromatoleum aromaticum EbN1
Azoarcus sp. EbN1 plasmid 1
DNA section
IS Length : 1158 bp

Ends


IR Length : 20/33

IRL : GAGGGCGCCTCGAAAAACCTCGTCCCGCCCACTCTTGGTAAAATTACAGC
IRR : CAGAATCGCCTCGGGCAGCGGCGATTCGGCCCATTTCGGGCGTCAGAAGG

Insertion site


Left flankDirect repeatRight flankDR Length
CGGCAACACATTACAACGGTCTT3

DNA sequence

GAGGGCGCCTCGAAAAACCTCGTCCCGCCCACTCTTGGTAAAATTACAGCGTCCTGAATCCTGCTGTTGCCTGCACCGATGAAACCGCGTAGCGCTATCA
AGACTGACCTGTTCGCTGACGAACACCACCGCAAGAAGCTCGACTCGCTGGGCGACCCGCTGGCCGACATCGAGTTGCACATCGACTTTGCGGCGCTGGC
CGCCGAGGTCGATCGGGTCGCACCGCGCACGGTCAGCGCGCAAGGCGGCCGTCCGCCTTACCCGACGGAGACGATGGTTCGGATCCTGGTGCTCAAGCGC
CTGTACAACCTGTCGGACGAGCAGATGGAGTACCAGCTGCTCGACCGCATGAGCTACAAACGCTTCTGCGGGCTCGCGAACGCAGCCAATATTCCGGACC
GCACCACGGTGTGGACCTTCGAGAACCGGATCGGTGAGCTTGGCGCGAAGGCGCTCTTCGAGGGCGTCTCGGCGCAGCTGCTCAAACGGGGCTTCATCGC
GCGTGGCGGGCAGATCATCGACGCCACCTTGGTGCCTGCCCCCAAGCAGCACAACAGCCGAGGGGAGAAGGCGCTGATCGACCAAGGCGCGATGCCTGCC
GACTGGAAGCCGGCCAAGCGCCGGCAGAAGGACACCGACGCCACCTGGACGAAGAAACACGGCAAGAGCCACTTCGGCTACAAGCTGTCGATCAACGTCG
ACAAGAAGTACAAATTCATCCGCACGCTCGAGACCGACACGGCCAGCACGCACGACAGCCAGCACTTCGACAACGTCTTCGACACGAGCAACACGAGCCG
CGACGTCTATGCCGATCGCGGCTATCCGTCCGAGGAGCGGGCGGCCTGGTTGAAGGCGAACGGCTTTCGGAATCAAATCCAGCGCAAGGGACAACGCAAC
AAGCCGCTGTCCGAATGCCAGCAGCGGCGGAACACGCGCATCGCGCGGACGCGAGCGCGGGTGGAACACCCGTTCGCGGCCATCGCGCAAATGGGCGGCA
AGTTGATCCGAACCATCGGCCAGGCGCGGGCGAGCTTCGCGATGACGATGATGGCAGCCTGCTACAACCTGAAGCGGCTGACGTATTTCCAGAAGGCCGG
AATCGTGGCCTTCTGACGCCCGAAATGGGCCGAATCGCCGCTGCCCGAGGCGATTCTG
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1038 bp345 aa791116+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MKPRSAIKTDLFADEHHRKKLDSLGDPLADIELHIDFAALAAEVDRVAPRTVSAQGGRPPYPTETMVRILVLKRLYNLSDEQMEYQLLDRMSYKRFCGLA
NAANIPDRTTVWTFENRIGELGAKALFEGVSAQLLKRGFIARGGQIIDATLVPAPKQHNSRGEKALIDQGAMPADWKPAKRRQKDTDATWTKKHGKSHFG
YKLSINVDKKYKFIRTLETDTASTHDSQHFDNVFDTSNTSRDVYADRGYPSEERAAWLKANGFRNQIQRKGQRNKPLSECQQRRNTRIARTRARVEHPFA
AIAQMGGKLIRTIGQARASFAMTMMAACYNLKRLTYFQKAGIVAF

 

Blast result :
Comments
ISAzo41 is 93% aa similar to ISAzo11.
References
1] Kuhner,S., Wohlbrand,L., Fritz,I., Wruck,W., Hultschig,C., Hufnagel,P., Kube,M., Reinhardt,R. and Rabus,R. (2005) J. Bacteriol. 187 (4), 1493-1503.

2] Rabus,R., Kube,M., Heider,J., Beck,A., Heitmann,K., Widdel,F. and Reinhardt,R. (2005) Arch. Microbiol. 183 (1), 27-36.

3] PROSCIENCE (2004) Direct Submission GenBank.

4] ISfinder annotation (2009).