ISAcma8

  • Family IS701
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_009925 ND Acaryochloris marina
Acaryochloris marina MBIC11017 plasmid pREB7
Acaryochloris marina MBIC11017 plasmid pREB4
Acaryochloris marina MBIC11017 plasmid pREB2
Acaryochloris marina MBIC11017
DNA section
IS Length : 1398 bp

Ends


IR Length : 32/35

IRL : TAACCCTCTTTTATCACTTTAGACAGATAAAAAATAGACTATTCTCAGTT
IRR : TATCCCTCTTTTATCACTTTAGACAGAGAGAAAATCAGGATGCTTGAGCA

Insertion site


Left flankDirect repeatRight flankDR Length
TTGCTGAAATGAATATACAGCTGGCGGCTAAGGTCTGGGCTAAATGTTACTTCTTTTTAA0
CTAGTGTCCTGAATAGAATGGCGACTGCTAAGCCAATGGATTCAGTCGATGGACGAGTCA0
TAAATTTTTTACCCTAGACCGTTCCCTCCAAGACTCACAAACTTCCATCACAGACACCCG0
CTAGTGTCCTGAATAGAATGGCGACTGCTATCTTGCAAGAGATCATCCAGGTTGACTTTC0
TGGTGAGGAGGATTCTACCTACAGCCACCATGAAGGATGCGCTGTTTATGATGCCGACGG0
TTTAAGTCCTTCTCAATATGGCGACTGCTATCCATCAAATCAGCAACAAGCTTGAGAT2
TCTGAAACAAGATTAGGTTGTTTGCAGATTAAGGTTCAGCCACTATGTCTTGGGCCCAGC0
AGAAAGTTAATCTCTTAACCGTCTAGAGTACAAAACCTAACTTCTCCAACTCGGAAGT2
CTAGCGTCCTGAATAGTATGGCGACTGCTATAGAAACAAGCATGCCAAAACACATCCT2
GTAAAGTCTGGCAACAACATGCAGTGACCTAGACTGGTGCTGATACCAATTACCCAGCTA0
TAGCGGTAAAAGGATGCTCTACTTTCATCTACTCTAGACGGTTAAGAGATTAACTTTCT0
ACTGACTTAAGCTCAATCAAACAGCTAGCTTACCAAATCCGACTTACTAACCCCCGAAAC0
TGACTTGTCACGCTAAGTCTCCTGGGTTGTTACTGCGTACGACTTGCGGCTTAACATTGT0
AGAAAGTTAATCTCTTAACCGTCTAGAGTAACTGCCATCCTTACTATCAGGAGATTTAGC0
TCAGAGTCACTTCTTGATATAGTCAAAATCTACTCTAGACGGTTAAGAGATTAACTTTCT0
AGAAAGTTAATATCTTAGCCGCCTAGAGTACTGTGATTATTAAGATTAAGACACCAATAA0
ATCGGATTTTAATCAAATTGAAATTTAATTTAAGGTGATGTTGGCGGGATTGCTGAGTGC0
AGAAAGTTAATATCTTAGCCGCCTAGAGTAGCTCTAAGGTTAAGACCCCTTGATCAAATG0
CTTTGCCAACTATGTAGGCCAGTCCCACCAAAGACCACCATTACGGGTGCCAAGATTG2

DNA sequence

TAACCCTCTTTTATCACTTTAGACAGATAAAAAATAGACTATTCTCAGTTTCTATTTTTCTAATGAGTGATGTCAGCTTCAAGAAGTCCAGAACCAACCA
TTGGCTTTGTTGATGAGTACTGCCAACTATTTGCAGAGTTATTTCCCCAAGTCCGCAGTTATGAAGCCTTCAAGCATCTCCATCTGGGGATGATTAGTGA
TATCAAACGTAAATCATTGCCCGCCATTGCAAGAGCAGTGGGCTTGGAGAACCATCAAAGCCTGCATCATTTTCTGAGTGAATCACCTTGGGTAGCGTCA
CATTTGTCTGACAAGAGATTGGACATCATCTTGAAAATACTGGATGGGCGCAGGTTAATTCTATTGATCGATGAGACGGGCGATTGCAAAAAAGGCAAGA
GTACCGACTATGTGAAACGGCAGTATATCGGCAATGTGGGCAAGAAAGAGAATGGCATCGTCGCAGTCACTGCCTACGGGCTGTTTCAAGGGATGATTTT
GCCTTTGTCCTTTGAAGTGTACAAACCCCGAGAACGTCTTAAAGAGGAGGATGAGTATCTCAGCAAACCCCAAATTGCTGCTGGAATGATCCGACAGTTG
CAAGGGATGGGCTTTTGCTTTGAGCTCGTCCTGGCTGACAGCTTGTATGGGGAAGCTCAAACAAACTTTGTGAATGTCTTAGAGGAGTTAAAGCTGCCCT
ATATCCTAGCGATTCGTAGCAATCATGCGGTGTGGTTACCCGCAGAACAAGCAGTCTTTGCCGAACCGTGGCAGTCATTTGAACGAACCTTCAGCAATGG
CACCACTGAAACTCGTTACAGGCAAGAGATCATTTATGGCCAGCGACACCACCAGAAGCGCTATTGGCTATTAACCACGGACCCCCAAACGCTACCAGAG
AACTCAACCTCTTATGTCATGGCAGCGGCACCCGAGATCAAGCTGGATGAAATTGGGGATTGCTATGGCTTCAGAACTTGGATTGAGTATGGTCTCAAAC
AGTCTAAAGATACCTTGGGTTGGGCTGATTTTCGCATGACGCACTACGAACAAATTGAAAAGTGGTGGGAGATAGTGATGAGCGCTTTCTTGATGGTGAG
TTTGTTCGCTGATGTCTTCAATGACTCTTGTCCAGTGGCACATCAACACTTTGCGCAGCATCCTTGGTGGGATAACCAAAACGGGTGGAAGAATCTGCTC
AATAATGTGCGCTTGATCATTCAACCGTTAATCAGTTGGAATCTTCTAAAGCGCTGGTTAGAAGTCTTTCCCAGCCGTGCTTTGAAAAAGGGGTTTGAGC
AACTCACATCAAAGATGGAGGCATTTTGCTGTCCACTGGTGAGGAAATTGCTCAAGCATCCTGATTTTCTCTCTGTCTAAAGTGATAAAAGAGGGATA
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1311 bp436 aa701380+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MSASRSPEPTIGFVDEYCQLFAELFPQVRSYEAFKHLHLGMISDIKRKSLPAIARAVGLENHQSLHHFLSESPWVASHLSDKRLDIILKILDGRRLILLI
DETGDCKKGKSTDYVKRQYIGNVGKKENGIVAVTAYGLFQGMILPLSFEVYKPRERLKEEDEYLSKPQIAAGMIRQLQGMGFCFELVLADSLYGEAQTNF
VNVLEELKLPYILAIRSNHAVWLPAEQAVFAEPWQSFERTFSNGTTETRYRQEIIYGQRHHQKRYWLLTTDPQTLPENSTSYVMAAAPEIKLDEIGDCYG
FRTWIEYGLKQSKDTLGWADFRMTHYEQIEKWWEIVMSAFLMVSLFADVFNDSCPVAHQHFAQHPWWDNQNGWKNLLNNVRLIIQPLISWNLLKRWLEVF
PSRALKKGFEQLTSKMEAFCCPLVRKLLKHPDFLSV

 

Blast result :
Comments
ISAcma8 is 71% aa similar to ISNpu5.
References
1] Swingley,W.D., Chen,M., Cheung,P.C., Conrad,A.L., Dejesa,L.C.,Hao,J., Honchak,B.M., Karbach,L.E., Kurdoglu,A., Lahiri,S.,Mastrian,S.D., Miyashita,H., Page,L., Ramakrishna,P., Satoh,S., Sattley,W.M., Shimada,Y., Taylor,H.L., Tomo,T., Tsuchiya,T., Wang,Z.T., Raymond,J., Mimuro,M., Blankenship,R.E. and Touchman,J.W. (2008) Proc. Natl. Acad. Sci. U.S.A.
2] ISfinder annotation (2009)