ISAcma7

  • Family IS256
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_009925 ND Acaryochloris marina
Acaryochloris marina MBIC11017 plasmid pREB2
Acaryochloris marina MBIC11017
Acaryochloris marina MBIC11017 plasmid pREB1
DNA section
IS Length : 1347 bp

Ends


IR Length : 24/28

IRL : GGGACTGTTCAGAGTTTTGTGTAAGCGGTCTAGGATCCAAACACATCAGG
IRR : GAGAGTGTTTAGATTTTTGTGTAAGCAGCAGTGTATTCAATAGTTGAATC

Insertion site


Left flankDirect repeatRight flankDR Length
TTACCGGAAGAGAATTAGATTTAACCTGAATTTCTCACAAGCAGTTATCAATG7
CCTATCAAGTTCAAGTAGTTCAAAAGCCGATGATAGTAACTGCCCATTACTGT7
CAAATAGGTTCTATAAAATCGCACTTTTGCCTTTGATATAACCCATCACATTT7
TAAAAATCGAGGAACTCAGGATATCTTGATTGCTTGCTGTGATGGACTCAAAGG6
AATCTTGTACCGAATGAGGGAAATTAATGTCATCAAAACGTCGTCAATATAGT7
AACAAAGCAAGCATCTGATTGAAGTGTTGTCGTGCTCCTTGCACAGACAGTTG7
AACAAAGTGTGGGATAGGAAGCTAAGTCTTGCCATCTCCAAGGACGCTCCGTC7
AACGATGGCTCTGGAAGATGAGGCTAGCTATTTCGCCTTGTCTTCCAAAACAG7

DNA sequence

GGGACTGTTCAGAGTTTTGTGTAAGCGGTCTAGGATCCAAACACATCAGGAGACCGTACGATGTCTAGAAAGAAAAAAGCTCCCAATCGAGTCGATGAAC
TGTTAGATGAACTCATCGGAGAGAACCCTCAACCGGAAGATATTCTGGGCGAATCAGGACTGCTTAAACGACTCAGCAAACGCCTAGTGGAACGAGCCCT
TGCAGGTGAATTGACTCATCACCTGCAGCAATCCTCCAACGATCAGTCAGGTGATGAGAATTCTAATGGCCCCAGAAATAGTCGCAATGGTTACTCGAAA
AAGACCGTCCAGTCTGAACAAGGAGAGATGGACCTATCGATTCCTCGTGACCGTTGCGGCGAGTTTGAGCCTGTCCTGGTGCCTAAAGGCCAGCGACGCA
TCGCAGGGCTCGATGAGAAAATCATCGCCTTATATGCTCGGGGCATGACCACCCGTGATATTCGAGCCCAATTGGTGGAGCTGTATGGGGCCAATATCTC
TGAAGCTCTGATTAGTGACGTCACCAATAGCGTCATGGAAGAGGTCAAAGACTGGCGTTCAAGGCCGCTGGATGAGGTGTATCCGATTGTCTATCTTGAT
GCGCTGTATGTGAACATCAAAGTCAATGGTCAAGTCAGCAAGCGAGCTGTCTATGTCGCTTTGGCAGTGACTGTGGAGGGCAACAAGGAACTGTTGGGAT
TATGGATAGGAGCCGCTGAACGAGAGGGAGCAAAATTCTGGCTTTCGGTGCTCACAGACCTTAAAAATCGAGGAACTCAGGATATCTTGATTGCTTGCTG
TGATGGACTCAAAGGTTTTCCTCAAGCGATTGAGTCGGTCTACCCTCAGACCCAGGTGCAGGTTTGTATTGTCCATCTTATTCGTAATAGCCTTCGCCAT
GTGCCGTGGAAGGAAAGTCGAGCCGTAGCTGCAGACCTCAAGCCCATTTACCAAGCGGCCACTTTGGAAGAGTCTGAGGCTGCGTTGGATGCATTTGCCC
ATAAATGGGACGAGACGTATCCTGGCATTAGCCAAATCTGGATACGACATTGGGATAATGTTGCCCCGTTGTTTCAATACCCAATGGCCATCCGTAAGGT
TATCTACACCACCAATGCCATTGAGTCGCTCAACCGCTCACTGAGAAAGGTGATTAAGACCAAAGCGGTTTTCCCTAATGAAGAGTCTGTCTACAAATTG
ATGTTTCTAGCGATGAGGAATATTTCGAAGCGATGGACCAGACCAATTCTGAATTGGAAGGCGGCGCTGTCCCATTTTGCTATTCTGTTCCCAACACGAT
TCAACTATTGAATACACTGCTGCTTACACAAAAATCTAAACACTCTC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1251 bp416 aa611311+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MSRKKKAPNRVDELLDELIGENPQPEDILGESGLLKRLSKRLVERALAGELTHHLQQSSNDQSGDENSNGPRNSRNGYSKKTVQSEQGEMDLSIPRDRCG
EFEPVLVPKGQRRIAGLDEKIIALYARGMTTRDIRAQLVELYGANISEALISDVTNSVMEEVKDWRSRPLDEVYPIVYLDALYVNIKVNGQVSKRAVYVA
LAVTVEGNKELLGLWIGAAEREGAKFWLSVLTDLKNRGTQDILIACCDGLKGFPQAIESVYPQTQVQVCIVHLIRNSLRHVPWKESRAVAADLKPIYQAA
TLEESEAALDAFAHKWDETYPGISQIWIRHWDNVAPLFQYPMAIRKVIYTTNAIESLNRSLRKVIKTKAVFPNEESVYKLMFLAMRNISKRWTRPILNWK
AALSHFAILFPTRFNY

 

Blast result :
Comments
ISAcma7 is 74% aa similar to ISSod18.
References
1] Swingley,W.D., Chen,M., Cheung,P.C., Conrad,A.L., Dejesa,L.C., Hao,J., Honchak,B.M., Karbach,L.E., Kurdoglu,A., Lahiri,S., Mastrian,S.D., Miyashita,H., Page,L., Ramakrishna,P., Satoh,S., Sattley,W.M., Shimada,Y., Taylor,H.L., Tomo,T., Tsuchiya,T., Wang,Z.T., Raymond,J., Mimuro,M., Blankenship,R.E. and Touchman,J.W. (2008) Proc. Natl. Acad. Sci. U.S.A.
2] ISfinder annotation (2009)