ISAzo40

  • Family IS1380
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_006823 ND Azoarcus sp.
Aromatoleum aromaticum EbN1
Azoarcus sp. EbN1 plasmid 1
DNA section
IS Length : 1694 bp

Ends


IR Length : 19/24

IRL : CCTGAATCCGTGTTCGCTTAGCCGGAAATCCAGCCTAACACATTGATGTA
IRR : CCTGAATCCGTGTTGTCTTCAGCGCGTACCCGCTCCGCGAGCCCCAGCGC

Insertion site


Left flankDirect repeatRight flankDR Length
CGATTCTATCAGTTAGCTTATGTCG5
CGAACAGTGCTATTTGCCGTATGGA5

DNA sequence

CCTGAATCCGTGTTCGCTTAGCCGGAAATCCAGCCTAACACATTGATGTAATTGTACAATTACGCATCCGACTCATTCACCCAACCGACCATGCCGCGCT
TCGAAGTCAAGCAATCCGCCAAGCTCAACCTGACCTCCTACTCGGGCTTGGCACTGATCGGGCAGTGCTGCCAGGCGGCACAGGTCGAGGCGGTGATCGA
CCCGAGGCTGCCGGTGTCGCAGGGCATGCGCACCTCGGACCTGGTCAAGTCGATGGTGGGGCTGCTGAGCCTGGGCAAGAGCGACTTCGAGGCGATCGAG
CCGTTTCGCAGCGATCGCTTCTTCAAGGAAGCGCTCGGGCTCGCCAAGGTGCCCGGCAGCGTGTGGATGCGCCAACGGCTCGATGCCCGCGCGGCCGAGC
TGCGCGAGCTGACCGACGAGCTGAGCCTGCGCCTGCTCGAGCGCACCGAGGCGCCGATCACGGCGCACAAGGGCTACGTGTGCGCCGACATGGATACCTT
CGTGATGGATAACTCCGACACCAAGAAGGAGGCGGTCAGCCGCACCTACCAGGGGGTCGACGGCTACACGCCGATCGCGCTGTACCTGGGCAACGAGGGC
TGGAACCTCGGCCTGGAGCTGCGCGCGGGTTCGCACCACTCGGCACTGGAGACGGAGTATTTCTTCGAGCGGGTCTTTCCGCGTCTCGAGCGCGTGTGCG
CCGCGGACGCGAAGGTGCTGTGGCGCGCCGACAGCGGCTTCGACAGCGCCCGGCTGTTGTTCGCGCTGGCGGCCGAGCGCGAGCGCTGGGCCGGTCTTGA
GCGCTCGTTCGATTACCTCACCAAGTGGAATCCGCGCCGTCAGGACAAGGCCGCCTGGGTCGCCCGCGCCGAGGCCGCCGGCGTCTTCGAGGAGGTCCGC
GCGGGCAAGCGCGTGGGACTGCTCGATCTGCAGGTGGAGCGCGCCTGGAAGAAGGCCAAGCGCACGCTGCGTCTGGTGGTGCGGGTGACCGAGCGCACGA
TCGACAAGAAGGGCCAGCACCTGCTGGCCCCCGAGATCGAGATCGAAGGCTGGTGGACCAGCCTTGATGTGCCGATGGCCGATGTGATCGAGCTCTACAA
GCACCACGGCACCCACGAGCAGTTCCACTCCGAGATCAAGACCGACCTGGACCTCGAGCGCCTGCCCTCGGGCAAGTTCGACACCAACGACGCGGTCGTG
CACCTGGCCGCATTCGCCTACAACTGCCTGCGCCTGATCGGGCAGCTCGGGCTGACCGGCGAGCTCTCGCCGATCCGCCACCCGGCCAAGCGCCGGCGCA
TCAAGACGGTGTTGCAGGAGGTGATGTACCGGGCGGCGAAGTTCGTCGAACACGCTCGCCGCCTGGTGCTGGACTTCGGGCGCGGCGTCGCCGCGCATGT
GAAGGTGTTCACCACGGTGCAGGCGCGGCTGTGTGTGGTGGCTTCGCCGTGATGCGAAGCCGGCGACTGAATTCTCCGAATCGAGGCTATTTCGCAGCGG
CGGAAGGGCGACGGCCGCGCGGGAGCAGGAAAATGACGTAGCGCCCCCGCCGGAAGCCCGGCTCACCGGCGCTCCGGGCCGTGAAAGGACGGCGCTCGGG
GCTGCGGTGAGCTGAGATTGGGAGTCAGGCAGCAGAAAAATCATGCGCTGGGGCTCGCGGAGCGGGTACGCGCTGAAGACAACACGGATTCAGG
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1362 bp453 aa911452+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MPRFEVKQSAKLNLTSYSGLALIGQCCQAAQVEAVIDPRLPVSQGMRTSDLVKSMVGLLSLGKSDFEAIEPFRSDRFFKEALGLAKVPGSVWMRQRLDAR
AAELRELTDELSLRLLERTEAPITAHKGYVCADMDTFVMDNSDTKKEAVSRTYQGVDGYTPIALYLGNEGWNLGLELRAGSHHSALETEYFFERVFPRLE
RVCAADAKVLWRADSGFDSARLLFALAAERERWAGLERSFDYLTKWNPRRQDKAAWVARAEAAGVFEEVRAGKRVGLLDLQVERAWKKAKRTLRLVVRVT
ERTIDKKGQHLLAPEIEIEGWWTSLDVPMADVIELYKHHGTHEQFHSEIKTDLDLERLPSGKFDTNDAVVHLAAFAYNCLRLIGQLGLTGELSPIRHPAK
RRRIKTVLQEVMYRAAKFVEHARRLVLDFGRGVAAHVKVFTTVQARLCVVASP

 

Blast result :
Comments
ISAzo40 is 62% aa similar to ISCARN34.
References
1] Kuhner,S., Wohlbrand,L., Fritz,I., Wruck,W., Hultschig,C., Hufnagel,P., Kube,M., Reinhardt,R. and Rabus,R. (2005) J. Bacteriol. 187 (4), 1493-1503.

2] Rabus,R., Kube,M., Heider,J., Beck,A., Heitmann,K., Widdel,F. and Reinhardt,R. (2005) Arch. Microbiol. 183 (1), 27-36.

3] PROSCIENCE (2004) Direct Submission GenBank.

4] ISfinder annotation (2009).