ISfinder Mirror Site

ISAzo38

Family IS5
Group IS427

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_006824	ND	Azoarcus sp.	Aromatoleum aromaticum EbN1 Azoarcus sp. EbN1 plasmid 2

DNA section

IS Length : 894 bp

Ends

IR Length : 15/17

IRL : GAACCTATCCCGGTAGGTCGACTGCCAAATCGCCGGGGGGTTGATTTGAG
IRR : GAACCTATTCCAGTAGGCCAGTGGCCCGCCAAGTGATGATGCCGCAGGCA

Insertion site

Left flank	Direct repeat	Right flank	DR Length
GGGATCCCAC	TAA	TACTACAGTT	3
ATGTTTCTCC	TAA	TACTACAGTT	3

DNA sequence

GAACCTATCCCGGTAGGTCGACTGCCAAATCGCCGGGGGGTTGATTTGAGAGGACACGGCGATGGGACTGGTCTGCAAGGATGACGGGTGGCGGCTGCCG
GACGAGTTGTGGGCGAAGATGGAGCCGTTGTTGCCAGCGCGCAAAGCGCACCCGCTGGGCTGTCATCGGCCGCGGGTGCCGGACCGAATCGCGATGAATG
CGATTCTGCTGGTACTGCGCACCGGGATGCAGTGGAACGCGCTCAACGGCAGCGGGATTTGCTCGAGCAGTTCGGCGTACCGGCGGTTTGCCGAATGGCG
CGATGCGGGCGTTTTTGGGCGCTTCTGGCACGAGGGTTTGCTCGAGTACGATCGCCTCGAGGGCATTGACTGGAGTTGGCTGTCGTTGGATGGGGCGATG
ACCAAAGCGCCGTTGGGTGGGGAAAAAAACCGGCCCCAACCCTACGGATCGCGGCAAAGGCGGCGTCAAGCGCAGTCTGTTGACCGAGGCGAGCGGCATT
CCGCTGGCCATTGAAATCGATGGCGCGAACCGCCACGACATGAAGCTGGTCGAGGCGACGCTGGGCAAGCTGATGGTCAAACGGCCAACCCCGAGCATGA
CACGACCGCAGGGCCTGTGCCTGGACAAGGGATACGACTACGACGAAGTGCGGGAGCTGGTAGCCGGCCTCGGATTTACTGCCCATATCCGCGCCCGCGG
CGAGGAACAGCGTGACATCCGGGAACTCGGCTATCGAGCGCGGCGGTGGGTCGTCGAGCGGACCCACGGCTGGCTCAATCGCTATCGTCGCCTGTTGATA
CGTTGGGAGAAGAAAGCCGACTCCTACCTCGCCATGCTGCACTTTGCCTGCGGCATCATCACTTGGCGGGCCACTGGCCTACTGGAATAGGTTC

Recoding section

Recoding by frameshift
Frame
Type
Experimentally demonstrated

Stimulators :

Shine-Dalgarno sequence :
Secondary structure :

Recoding motif :

Protein section

ORF number : 3

ORF 1

Length		Begin	End	Strand	Fusion ORF
453 bp	150 aa	62	514	+	No

ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MGLVCKDDGWRLPDELWAKMEPLLPARKAHPLGCHRPRVPDRIAMNAILLVLRTGMQWNALNGSGICSSSSAYRRFAEWRDAGVFGRFWHEGLLEYDRLE
GIDWSWLSLDGAMTKAPLGGEKNRPQPYGSRQRRRQAQSVDRGERHSAGH

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
489 bp	162 aa	402	890	+	No

ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

PKRRWVGKKTGPNPTDRGKGGVKRSLLTEASGIPLAIEIDGANRHDMKLVEATLGKLMVKRPTPSMTRPQGLCLDKGYDYDEVRELVAGLGFTAHIRARG
EEQRDIRELGYRARRWVVERTHGWLNRYRRLLIRWEKKADSYLAMLHFACGIITWRATGLLE

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
829 bp	275 aa	62	890	+	Yes

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MGLVCKDDGWRLPDELWAKMEPLLPARKAHPLGCHRPRVPDRIAMNAILLVLRTGMQWNALNGSGICSSSSAYRRFAEWRDAGVFGRFWHEGLLEYDRLE
GIDWSWLSLDGAMTKAPLGGEKTGPNPTDRGKGGVKRSLLTEASGIPLAIEIDGANRHDMKLVEATLGKLMVKRPTPSMTRPQGLCLDKGYDYDEVRELV
AGLGFTAHIRARGEEQRDIRELGYRARRWVVERTHGWLNRYRRLLIRWEKKADSYLAMLHFACGIITWRATGLLE

Blast result :

Comments

ISAzo38 is 71% aa similar to ISMac11.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible +1 frameshift.

References

1] Kuhner,S., Wohlbrand,L., Fritz,I., Wruck,W., Hultschig,C., Hufnagel,P., Kube,M., Reinhardt,R. and Rabus,R. (2005) J. Bacteriol. 187 (4), 1493-1503.

2] Rabus,R., Kube,M., Heider,J., Beck,A., Heitmann,K., Widdel,F. and Reinhardt,R. (2005) Arch. Microbiol. 183 (1), 27-36.

3] PROSCIENCE (2004) Direct Submission GenBank.

4] ISfinder annotation (2009).