ISAzo37
- Family IS5
- Group IS1031
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_006824 | ND | Azoarcus sp. | Aromatoleum aromaticum EbN1 Azoarcus sp. EbN1 plasmid 2 |
DNA section
IS Length : 843 bp
Ends
IR Length : 17
IRL : GAACCTGTTCAAAGTCTCGGCGGGATAGCGGATACTGGCGGGATGAGACA
IRR : GAACCTGTTCAAAGTCTTTTGAGCATGAGGCAGAGGAAGGCCAAGTGGAT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GCTCGATCCC | TTA | GGGAAATTTT | 3 |
DNA sequence
GAACCTGTTCAAAGTCTCGGCGGGATAGCGGATACTGGCGGGATGAGACAGTGCTACCCGAGTGATATCAGCCATGAGCAGTTCGAGCAGATTCGCTCGC
TGCTGGAGAGTGCGCGCAGGAAGACGGCGCCACGCAAGGTCGATCTGTACGATGTGTTCTGCGCGGTGCTGTACCTGTTGCGTACGGGCTGTCAGTGGCG
CGCCTTGCCGAGCGACTTTCCGAAGTGGCGCACCGTGCACTCGTACTTCGCCATCTGGAGCGAATTGCGCGAGGGTGGCAGTCTGCTCGAGCAGGCGCTA
AAAAAATCAGGTTGGCGAGGCCCGCGAGAGACTGGGGCGCAACGCAGGCAGCACGCTGTTGATCGTGGACGCGCAGAGCGTGAAGAACACGGACACGGCG
GCGAACAAGGGCTATGACGCCGGTAAGAAGGTCTCGGGGATCAAGCGCCACATCGCCGTCGACACCCGAGGTCTGCCGCACGCGGTGGCGGTGAGCACGG
CCGAGGTGACCGACCGCAAAGGGGCGCTGCTGGCGCTGAGTCGATGCAAAGCCGGGCTCGATCGGGTGCAAAGCGTGCTGGCCGATAGCGGTTATGTCGG
AGGACCCTTCGCGCAGGGCGTGCGCGAGATCCTCGGCGAGCACGTGACGGTGCAGATCGCCAAGCGCAGCGAACTGCACACCTTCAAGGTGATCCCCAAG
CGCTGGGTCGTCGAGCGCAGCTTTGCCTGGTTGGACAAGAACCGGCGGCTGTGGAAGAACTGCGAGCGGCTCCTGAACACCAGTCTGCAGTTCATCCACT
TGGCCTTCCTCTGCCTCATGCTCAAAAGACTTTGAACAGGTTC
TGCTGGAGAGTGCGCGCAGGAAGACGGCGCCACGCAAGGTCGATCTGTACGATGTGTTCTGCGCGGTGCTGTACCTGTTGCGTACGGGCTGTCAGTGGCG
CGCCTTGCCGAGCGACTTTCCGAAGTGGCGCACCGTGCACTCGTACTTCGCCATCTGGAGCGAATTGCGCGAGGGTGGCAGTCTGCTCGAGCAGGCGCTA
AAAAAATCAGGTTGGCGAGGCCCGCGAGAGACTGGGGCGCAACGCAGGCAGCACGCTGTTGATCGTGGACGCGCAGAGCGTGAAGAACACGGACACGGCG
GCGAACAAGGGCTATGACGCCGGTAAGAAGGTCTCGGGGATCAAGCGCCACATCGCCGTCGACACCCGAGGTCTGCCGCACGCGGTGGCGGTGAGCACGG
CCGAGGTGACCGACCGCAAAGGGGCGCTGCTGGCGCTGAGTCGATGCAAAGCCGGGCTCGATCGGGTGCAAAGCGTGCTGGCCGATAGCGGTTATGTCGG
AGGACCCTTCGCGCAGGGCGTGCGCGAGATCCTCGGCGAGCACGTGACGGTGCAGATCGCCAAGCGCAGCGAACTGCACACCTTCAAGGTGATCCCCAAG
CGCTGGGTCGTCGAGCGCAGCTTTGCCTGGTTGGACAAGAACCGGCGGCTGTGGAAGAACTGCGAGCGGCTCCTGAACACCAGTCTGCAGTTCATCCACT
TGGCCTTCCTCTGCCTCATGCTCAAAAGACTTTGAACAGGTTC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 790 bp | 264 aa | 43 | 832 | + | No |
Chemistry : DDE
ORF sequence :
MRQCYPSDISHEQFEQIRSLLESARRKTAPRKVDLYDVFCAVLYLLRTGCQWRALPSDFPKWRTVHSYFAIWSELREGGSLLEQALKKSGWRGPRETGAQ
RAGSTLLIVDAQSVKNTDTAANKGYDAGKKVSGIKRHIAVDTRGLPHAVAVSTAEVTDRKGALLALSRCKAGLDRVQSVLADSGYVGGPFAQGVREILGE
HVTVQIAKRSELHTFKVIPKRWVVERSFAWLDKNRRLWKNCERLLNTSLQFIHLAFLCLMLKRL
RAGSTLLIVDAQSVKNTDTAANKGYDAGKKVSGIKRHIAVDTRGLPHAVAVSTAEVTDRKGALLALSRCKAGLDRVQSVLADSGYVGGPFAQGVREILGE
HVTVQIAKRSELHTFKVIPKRWVVERSFAWLDKNRRLWKNCERLLNTSLQFIHLAFLCLMLKRL
Blast result :
Comments
The ORF has been reconstructed in silico because there is a mutation generating a frameshift at position 344 in the DNA sequence.
ISAzo37 is 92% aa similar to ISNieu4 and 86% aa similar to IS1480a.
ISAzo37 is 92% aa similar to ISNieu4 and 86% aa similar to IS1480a.
References
1] Kuhner,S., Wohlbrand,L., Fritz,I., Wruck,W., Hultschig,C., Hufnagel,P., Kube,M., Reinhardt,R. and Rabus,R. (2005) J. Bacteriol. 187 (4), 1493-1503.
2] Rabus,R., Kube,M., Heider,J., Beck,A., Heitmann,K., Widdel,F. and Reinhardt,R. (2005) Arch. Microbiol. 183 (1), 27-36.
3] PROSCIENCE (2004) Direct Submission GenBank.
4] ISfinder annotation (2009).
2] Rabus,R., Kube,M., Heider,J., Beck,A., Heitmann,K., Widdel,F. and Reinhardt,R. (2005) Arch. Microbiol. 183 (1), 27-36.
3] PROSCIENCE (2004) Direct Submission GenBank.
4] ISfinder annotation (2009).