ISAzo36

  • Family IS5
  • Group IS1031
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_006824 ND Azoarcus sp.
Aromatoleum aromaticum EbN1
Azoarcus sp. EbN1 plasmid 2
DNA section
IS Length : 817 bp

Ends


IR Length : 23/26

IRL : GAACCTGTTCAAAGTCTCTGGAGTAATGAGAACGTTGAGGGATGAGGAAG
IRR : GAACCTGTTCAAAGTCTATTGAGCAAAAGGGCCAGGAATGCGAGGTGGAT

Insertion site


Left flankDirect repeatRight flankDR Length
AGGCGCCTAATCAGGAATTC0

DNA sequence

GAACCTGTTCAAAGTCTCTGGAGTAATGAGAACGTTGAGGGATGAGGAAGAGCTATCCGAGCGCCATCAGCCGCGAACAGTTCGAGATCATCCGTCCGCT
GCTGGAGAGTGCGCGCAGGAAGACCTGTCCGAGGCGCGTGGACCTGTACGAAGTGTTCTGCGCGGTGTTGTATCTGCTCAGGAGTGGCTGCCAGGGGCGC
ATGCTGCCCGACGGGTTTCCCAAATGGCGCCCGGTGCACGCGTACTTTGCCATCTGGAGCGAGCCGCGCGAGGGTGGGAGCCTGCTGGAGCAGGCTTTAA
AAAATCAGGTTGGCGCAGCCCGGCAGAGACTGGAGCGCAACGCCTGCAGCACGGTCGTGATCGTGGACGCGCAAAGCGTGAAGAACACGGATACGGCGGT
CCAGAAGGGCTATGACGCGGGCAAGAAGGTGTCAGGGATCGAGCGCCACATTGCGGTCGACACCCAAGGCTTGCCGTATGCGAGGGCGGTGAGCACGGCT
GAGGTGACGGACCGCAAGGGGGGGCTGCAGGCGGCTGCGTCGTTGCAAGCCTGGGCTCGAGCGGGTGCAACGCGTGCTGTGCGACAGCGGCTATAGGGGC
CAGCCCTTCGCGCAGGGCGTGACGGTGCAGATCGCCAAGCGCCGCGAACCACCGCGTTCAAGGTGATGCCCAAGCGCTGGATCGTCGAGCGCAGCTTTGC
CTGGCTGGAAAAAAACAGGCGACTATGGGAGAAGTGCGAGCGGCTGCTCAACACCAGCTTGCAGTTCATCCACCTCGCATTCCTGGCCCTTTTGCTCAAT
AGACTTTGAACAGGTTC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
768 bp255 aa42809+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MRKSYPSAISREQFEIIRPLLESARRKTCPRRVDLYEVFCAVLYLLRSGCQGRMLPDGFPKWRPVHAYFAIWSEPREGGSLLEQALKNQVGAARQRLERN
ACSTVVIVDAQSVKNTDTAVQKGYDAGKKVSGIERHIAVDTQGLPYARAVSTAEVTDRKGGLQAAASLQAWARAGATRAVRQRL*GPALRAGRDGADRQA
PRTTAFKVMPKRWIVERSFAWLEKNRRLWEKCERLLNTSLQFIHLAFLALLLNRL

 

Blast result :
Comments
There is a STOP codon in frame, the transposase is probably inactive.
ISAzo36 is 74% aa similar to IS1480b.
References
1] Kuhner,S., Wohlbrand,L., Fritz,I., Wruck,W., Hultschig,C., Hufnagel,P., Kube,M., Reinhardt,R. and Rabus,R. (2005) J. Bacteriol. 187 (4), 1493-1503.

2] Rabus,R., Kube,M., Heider,J., Beck,A., Heitmann,K., Widdel,F. and Reinhardt,R. (2005) Arch. Microbiol. 183 (1), 27-36.

3] PROSCIENCE (2004) Direct Submission GenBank.

4] ISfinder annotation (2008).