ISAzo36
- Family IS5
- Group IS1031
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_006824 | ND | Azoarcus sp. | Aromatoleum aromaticum EbN1 Azoarcus sp. EbN1 plasmid 2 |
DNA section
IS Length : 817 bp
Ends
IR Length : 23/26
IRL : GAACCTGTTCAAAGTCTCTGGAGTAATGAGAACGTTGAGGGATGAGGAAG
IRR : GAACCTGTTCAAAGTCTATTGAGCAAAAGGGCCAGGAATGCGAGGTGGAT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| AGGCGCCTAA | TCAGGAATTC | 0 |
DNA sequence
GAACCTGTTCAAAGTCTCTGGAGTAATGAGAACGTTGAGGGATGAGGAAGAGCTATCCGAGCGCCATCAGCCGCGAACAGTTCGAGATCATCCGTCCGCT
GCTGGAGAGTGCGCGCAGGAAGACCTGTCCGAGGCGCGTGGACCTGTACGAAGTGTTCTGCGCGGTGTTGTATCTGCTCAGGAGTGGCTGCCAGGGGCGC
ATGCTGCCCGACGGGTTTCCCAAATGGCGCCCGGTGCACGCGTACTTTGCCATCTGGAGCGAGCCGCGCGAGGGTGGGAGCCTGCTGGAGCAGGCTTTAA
AAAATCAGGTTGGCGCAGCCCGGCAGAGACTGGAGCGCAACGCCTGCAGCACGGTCGTGATCGTGGACGCGCAAAGCGTGAAGAACACGGATACGGCGGT
CCAGAAGGGCTATGACGCGGGCAAGAAGGTGTCAGGGATCGAGCGCCACATTGCGGTCGACACCCAAGGCTTGCCGTATGCGAGGGCGGTGAGCACGGCT
GAGGTGACGGACCGCAAGGGGGGGCTGCAGGCGGCTGCGTCGTTGCAAGCCTGGGCTCGAGCGGGTGCAACGCGTGCTGTGCGACAGCGGCTATAGGGGC
CAGCCCTTCGCGCAGGGCGTGACGGTGCAGATCGCCAAGCGCCGCGAACCACCGCGTTCAAGGTGATGCCCAAGCGCTGGATCGTCGAGCGCAGCTTTGC
CTGGCTGGAAAAAAACAGGCGACTATGGGAGAAGTGCGAGCGGCTGCTCAACACCAGCTTGCAGTTCATCCACCTCGCATTCCTGGCCCTTTTGCTCAAT
AGACTTTGAACAGGTTC
GCTGGAGAGTGCGCGCAGGAAGACCTGTCCGAGGCGCGTGGACCTGTACGAAGTGTTCTGCGCGGTGTTGTATCTGCTCAGGAGTGGCTGCCAGGGGCGC
ATGCTGCCCGACGGGTTTCCCAAATGGCGCCCGGTGCACGCGTACTTTGCCATCTGGAGCGAGCCGCGCGAGGGTGGGAGCCTGCTGGAGCAGGCTTTAA
AAAATCAGGTTGGCGCAGCCCGGCAGAGACTGGAGCGCAACGCCTGCAGCACGGTCGTGATCGTGGACGCGCAAAGCGTGAAGAACACGGATACGGCGGT
CCAGAAGGGCTATGACGCGGGCAAGAAGGTGTCAGGGATCGAGCGCCACATTGCGGTCGACACCCAAGGCTTGCCGTATGCGAGGGCGGTGAGCACGGCT
GAGGTGACGGACCGCAAGGGGGGGCTGCAGGCGGCTGCGTCGTTGCAAGCCTGGGCTCGAGCGGGTGCAACGCGTGCTGTGCGACAGCGGCTATAGGGGC
CAGCCCTTCGCGCAGGGCGTGACGGTGCAGATCGCCAAGCGCCGCGAACCACCGCGTTCAAGGTGATGCCCAAGCGCTGGATCGTCGAGCGCAGCTTTGC
CTGGCTGGAAAAAAACAGGCGACTATGGGAGAAGTGCGAGCGGCTGCTCAACACCAGCTTGCAGTTCATCCACCTCGCATTCCTGGCCCTTTTGCTCAAT
AGACTTTGAACAGGTTC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 768 bp | 255 aa | 42 | 809 | + | No |
Chemistry : DDE
ORF sequence :
MRKSYPSAISREQFEIIRPLLESARRKTCPRRVDLYEVFCAVLYLLRSGCQGRMLPDGFPKWRPVHAYFAIWSEPREGGSLLEQALKNQVGAARQRLERN
ACSTVVIVDAQSVKNTDTAVQKGYDAGKKVSGIERHIAVDTQGLPYARAVSTAEVTDRKGGLQAAASLQAWARAGATRAVRQRL*GPALRAGRDGADRQA
PRTTAFKVMPKRWIVERSFAWLEKNRRLWEKCERLLNTSLQFIHLAFLALLLNRL
ACSTVVIVDAQSVKNTDTAVQKGYDAGKKVSGIERHIAVDTQGLPYARAVSTAEVTDRKGGLQAAASLQAWARAGATRAVRQRL*GPALRAGRDGADRQA
PRTTAFKVMPKRWIVERSFAWLEKNRRLWEKCERLLNTSLQFIHLAFLALLLNRL
Blast result :
Comments
There is a STOP codon in frame, the transposase is probably inactive.
ISAzo36 is 74% aa similar to IS1480b.
ISAzo36 is 74% aa similar to IS1480b.
References
1] Kuhner,S., Wohlbrand,L., Fritz,I., Wruck,W., Hultschig,C., Hufnagel,P., Kube,M., Reinhardt,R. and Rabus,R. (2005) J. Bacteriol. 187 (4), 1493-1503.
2] Rabus,R., Kube,M., Heider,J., Beck,A., Heitmann,K., Widdel,F. and Reinhardt,R. (2005) Arch. Microbiol. 183 (1), 27-36.
3] PROSCIENCE (2004) Direct Submission GenBank.
4] ISfinder annotation (2008).
2] Rabus,R., Kube,M., Heider,J., Beck,A., Heitmann,K., Widdel,F. and Reinhardt,R. (2005) Arch. Microbiol. 183 (1), 27-36.
3] PROSCIENCE (2004) Direct Submission GenBank.
4] ISfinder annotation (2008).