ISfinder Mirror Site

ISAzo35

Family IS701
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_006824	ND	Azoarcus sp.	Aromatoleum aromaticum EbN1 Azoarcus sp. EbN1 plasmid 2

DNA section

IS Length : 1451 bp

Ends

IR Length : 21/24

IRL : TACTACAGTTGCAGATCATTCTTGTCCGACGCCTGTCAAAGCGAGGTTGG
IRR : TACTACAGTTGCAGATGCTCTTGAATTACACGGCGGTAATGACAGTCCTT

Insertion site

Left flank	Direct repeat	Right flank	DR Length
TTGTGTGTTG	TTAG	AACCTATTCC	4
TAGGTTCTAA		CTAGAGATCG	0

DNA sequence

TACTACAGTTGCAGATCATTCTTGTCCGACGCCTGTCAAAGCGAGGTTGGCTGCAGTCGAGGGTGGCAGTGATGTTGAGGCAGGCGGTTGAGTTGGCGCC
CCACCGGGCGTTCTCCGAAGTGGAGCGCTGGTCAGAGCAGTTCGAAGCGCTGGTGCAGGAGATCGGGTGGCGTTTTTCGCGCAAGGATGTTCGCCGACAT
GCCATGGATTATTTGCGTGGCCTGCTCGGACCGGCCGAGCGCAAGAACGGCTGGCAGTTAGCCGAAGCGGCCGGTGAGGCAAGCCCGGCGGCGATCCAGC
ATTTGCTCGGGCGCGCGGTGTGGAATGCCGACGCGGTCCGCGACGATCTGCGCACCTATGTGACGAGGCATCTGAAAGATCCCGAAGCGGTATTGGTCGT
CGATGAGACGGGCTTTCTCAAGAAGGGGCGCCACTCGGTCGGGGTGCAGCGCCAGTACAGCGGCACGGCCGGGCGCATCGAGAACTGCCAGATCGGCGTC
TTTCTCGCCTATGCCGGCGAGCACGGGCGCGCCTTCATCGACCGCGAGTTGTACCTGCCGCGCGAATGGACGGACGATGCGGCGCGCTGCGAAGCGGCCG
GTGTACCCAGGGACACCGGCTTTGCCACCAAACCGCAGCTGGCCCGGCGCCTGATCGAGGGCGCCATCGAGGCGAGCACCCCTTCGCCTGGGTAGCGGGT
GATGAGGTGTACGGCAATGACCGGCGCCTGCGGGTGTGGCTCGAGGAGCAGGATCGTTCCTATGTGCTGGCCATTGCCCGCACCCAGCATCTTTGGCGCG
AGACTATGCGCCAGCAAAGAGCGCGGGCCATCGCGCAGGAGATTCCCGACTCAGGGTGGCAACGACTGTCGGCCGGCGATGGCGCCAAGGGGCCGCGTCT
GTACGACTGGGCCTTTGTGCCGATGCTCAGTTGGATGACGTCCCAGCAGCGGGGCCTGCTGGTGCGCAGAAGCCTGGGTGAGGACCGGGAGCTGGCCTAC
TATGCGGTCTATGCCCCCTTCGGCACCGCGCTGCCCGAGCTGGTGCGCGTGGCCGGACGGCGCTGGGCGATCGAGGAAGGGTTCGAAGCGAGCAAACAGC
TCGTGGGCCTCGACCACTATGAGGTGCGCAGTTGGACCGGATAGTACCGGCACATCACACTGGCCCTACTCGCCCACGCCTATTTGGCTGTGCTGCGCCG
GGCGGCGATCGACTTCGACCTCAAAAAAAGTCCCGCCCCTCCGCGCTCCTCGCTGCAGGCGTTCAAGGCCCGGCGGGGCTGCTACCCCTGACCGTGCCCG
AGATCCGGCGCCTGCTGCTTGCCCTGGTCTGGCACCGCCCGCCTCAACCGGCCGCAGCCCTCGCCTGGTCCTACTGGCGACGACGACACCAAGCCTATGC
CAAGGACTGTCATTACCGCCGTGTAATTCAAGAGCATCTGCAACTGTAGTA

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
1220 bp	406 aa	72	1291	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MLRQAVELAPHRAFSEVERWSEQFEALVQEIGWRFSRKDVRRHAMDYLRGLLGPAERKNGWQLAEAAGEASPAAIQHLLGRAVWNADAVRDDLRTYVTRH
LKDPEAVLVVDETGFLKKGRHSVGVQRQYSGTAGRIENCQIGVFLAYAGEHGRAFIDRELYLPREWTDDAARCEAAGVPRDTGFATKPQLARRLIEGAIE
ASTPFAWVAGDEVYGNDRRLRVWLEEQDRSYVLAIARTQHLWRETMRQQRARAIAQEIPDSGWQRLSAGDGAKGPRLYDWAFVPMLSWMTSQQRGLLVRR
SLGEDRELAYYAVYAPFGTALPELVRVAGRRWAIEEGFEASKQLVGLDHYEVRSWTG*YRHITLALLAHAYLAVLRRAAIDFDLKKSPAPPRSSLQAFKA
RRGCYP

Blast result :

Comments

The protein sequence of ISAzo35 is degenerate and was reconstructed in silico: deletion of a C at position 681 and substitution G-1143-A
There is a STOP codon in frame, the transposase is probably inactive.
ISAzo35 is 68% aa similar to ISCARN75.

References

1] Kuhner,S., Wohlbrand,L., Fritz,I., Wruck,W., Hultschig,C., Hufnagel,P., Kube,M., Reinhardt,R. and Rabus,R. (2005) J. Bacteriol. 187 (4), 1493-1503.

2] Rabus,R., Kube,M., Heider,J., Beck,A., Heitmann,K., Widdel,F. and Reinhardt,R. (2005) Arch. Microbiol. 183 (1), 27-36.

3] PROSCIENCE (2004) Direct Submission GenBank.

4] ISfinder annotation (2008).