ISAzo33
- Family IS630
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_006824 | ND | Azoarcus sp. | Azoarcus sp. EbN1 plasmid 2 |
DNA section
IS Length : 1100 bp
Ends
IR Length : 18/24
IRL : CTAACTTTCCCGAAAGTAATGCCATATGGGCGATAATATGCGGCATGAAA
IRR : CTAACTTTTCAATAAGTAGTGTCACTATAGAGCGTATTGACACTCTGGTT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TGCTAGCAGA | TA | AGGCGGAGGC | 2 |
| CGGCGCCAGA | TA | GCAAGGCGCG | 2 |
DNA sequence
CTAACTTTCCCGAAAGTAATGCCATATGGGCGATAATATGCGGCATGAAATGCAAGCGAACTTCCGACGGTCGCGCCCACGATCATCACACCTTGCAGGT
GATGCGGCAGCAGGCCATCAAAGCGGTGCGTGAGGGGCAGACCGTGCAGAGCGTCGCCGCCTCGTTCGGCGTGAATCCTCGCAGCGTCTTCCGCTGGCTG
GCCGACTTCGCCAACGGCGGCCAGAACGCCCTGCTCGCCAAGCCGATCCCGGGACGTCCGCCCAGGCTCAGCGCCGAGGAGATGGCCTGGCTCGCCCGCG
CGGTCCGCGACCACACCCCGCAGCAGTTCAAGTTCGAGTTCGGGCTGTGGACGCTGACGCTGATCCGTCACCTGATCAATCGTCAGCTCAAGAAGGAACT
GTCGGTTTCCTCGGTCCACCGGCTCATGAAGATCCTGGGCTTCAGCGCCCAGAAACCGCTCTATCAGGCCTGGCAGCAGGATCCCGTGCTGGTGCGGACC
TGGGAGACCGAGACCTATCCCGCCATTCGCGCCGAAGCGCGTGCGACGGGCGCGACGATCTACTTTGCCGACGAGTCGGGAATCCGTTCCGACTACCACA
CCGGCACCCCCTGGGCGCCGCAGGGCCAGACGCCGGTGGTGACGGCCACCGGCCGGCGCTTTTCGCTGAACATGATCTCGGCGGTGAGCGCGCAGGGCGA
GTTTCGGTTCATGCTGCATGAAGGCTCGGTCGGTGCGAAGGTCTTTCTGGAGTTCCTCAAGCGCTTGATGATCGGCGCTCAGAAGCCGGTGTTCGTCATC
GTCGATGGTCATCCGATCCACAAGGCAAAGTTGATCAAGACCTATGTCGACAGCCTCGGCGGCCGGCTGAAGCTGTTCTACCTGCCGCCGTATTCGCCGC
ACCTGAACCCGGACGAAACGGTCTGGGCGCAGGTGAAGCGCAAGGTTTCGCGCCAGGTGGTCGAGAGCAAGGAGGACATGAAGCGCCTCGCACTCGGGGC
CCTGCGCAGCATTCAGAAACTCCCCGAGCTCGTGAAGTCATTCTTCCGGCAACCAGAGTGTCAATACGCTCTATAGTGACACTACTTATTGAAAAGTTAG
GATGCGGCAGCAGGCCATCAAAGCGGTGCGTGAGGGGCAGACCGTGCAGAGCGTCGCCGCCTCGTTCGGCGTGAATCCTCGCAGCGTCTTCCGCTGGCTG
GCCGACTTCGCCAACGGCGGCCAGAACGCCCTGCTCGCCAAGCCGATCCCGGGACGTCCGCCCAGGCTCAGCGCCGAGGAGATGGCCTGGCTCGCCCGCG
CGGTCCGCGACCACACCCCGCAGCAGTTCAAGTTCGAGTTCGGGCTGTGGACGCTGACGCTGATCCGTCACCTGATCAATCGTCAGCTCAAGAAGGAACT
GTCGGTTTCCTCGGTCCACCGGCTCATGAAGATCCTGGGCTTCAGCGCCCAGAAACCGCTCTATCAGGCCTGGCAGCAGGATCCCGTGCTGGTGCGGACC
TGGGAGACCGAGACCTATCCCGCCATTCGCGCCGAAGCGCGTGCGACGGGCGCGACGATCTACTTTGCCGACGAGTCGGGAATCCGTTCCGACTACCACA
CCGGCACCCCCTGGGCGCCGCAGGGCCAGACGCCGGTGGTGACGGCCACCGGCCGGCGCTTTTCGCTGAACATGATCTCGGCGGTGAGCGCGCAGGGCGA
GTTTCGGTTCATGCTGCATGAAGGCTCGGTCGGTGCGAAGGTCTTTCTGGAGTTCCTCAAGCGCTTGATGATCGGCGCTCAGAAGCCGGTGTTCGTCATC
GTCGATGGTCATCCGATCCACAAGGCAAAGTTGATCAAGACCTATGTCGACAGCCTCGGCGGCCGGCTGAAGCTGTTCTACCTGCCGCCGTATTCGCCGC
ACCTGAACCCGGACGAAACGGTCTGGGCGCAGGTGAAGCGCAAGGTTTCGCGCCAGGTGGTCGAGAGCAAGGAGGACATGAAGCGCCTCGCACTCGGGGC
CCTGCGCAGCATTCAGAAACTCCCCGAGCTCGTGAAGTCATTCTTCCGGCAACCAGAGTGTCAATACGCTCTATAGTGACACTACTTATTGAAAAGTTAG
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1032 bp | 343 aa | 45 | 1076 | + | No |
Chemistry : DDE
ORF sequence :
MKCKRTSDGRAHDHHTLQVMRQQAIKAVREGQTVQSVAASFGVNPRSVFRWLADFANGGQNALLAKPIPGRPPRLSAEEMAWLARAVRDHTPQQFKFEFG
LWTLTLIRHLINRQLKKELSVSSVHRLMKILGFSAQKPLYQAWQQDPVLVRTWETETYPAIRAEARATGATIYFADESGIRSDYHTGTPWAPQGQTPVVT
ATGRRFSLNMISAVSAQGEFRFMLHEGSVGAKVFLEFLKRLMIGAQKPVFVIVDGHPIHKAKLIKTYVDSLGGRLKLFYLPPYSPHLNPDETVWAQVKRK
VSRQVVESKEDMKRLALGALRSIQKLPELVKSFFRQPECQYAL
LWTLTLIRHLINRQLKKELSVSSVHRLMKILGFSAQKPLYQAWQQDPVLVRTWETETYPAIRAEARATGATIYFADESGIRSDYHTGTPWAPQGQTPVVT
ATGRRFSLNMISAVSAQGEFRFMLHEGSVGAKVFLEFLKRLMIGAQKPVFVIVDGHPIHKAKLIKTYVDSLGGRLKLFYLPPYSPHLNPDETVWAQVKRK
VSRQVVESKEDMKRLALGALRSIQKLPELVKSFFRQPECQYAL
Blast result :
Comments
ISAzo33 is 89% aa similar to ISAzo32.
References
1] Kuhner,S., Wohlbrand,L., Fritz,I., Wruck,W., Hultschig,C., Hufnagel,P., Kube,M., Reinhardt,R. and Rabus,R. (2005) J. Bacteriol. 187 (4), 1493-1503.
2] Rabus,R., Kube,M., Heider,J., Beck,A., Heitmann,K., Widdel,F. and Reinhardt,R. (2005) Arch. Microbiol. 183 (1), 27-36.
3] PROSCIENCE (2004) Direct Submission GenBank.
4] ISfinder annotation (2008).
2] Rabus,R., Kube,M., Heider,J., Beck,A., Heitmann,K., Widdel,F. and Reinhardt,R. (2005) Arch. Microbiol. 183 (1), 27-36.
3] PROSCIENCE (2004) Direct Submission GenBank.
4] ISfinder annotation (2008).