ISAzo33

  • Family IS630
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_006824 ND Azoarcus sp.
Azoarcus sp. EbN1 plasmid 2
DNA section
IS Length : 1100 bp

Ends


IR Length : 18/24

IRL : CTAACTTTCCCGAAAGTAATGCCATATGGGCGATAATATGCGGCATGAAA
IRR : CTAACTTTTCAATAAGTAGTGTCACTATAGAGCGTATTGACACTCTGGTT

Insertion site


Left flankDirect repeatRight flankDR Length
TGCTAGCAGATAAGGCGGAGGC2
CGGCGCCAGATAGCAAGGCGCG2

DNA sequence

CTAACTTTCCCGAAAGTAATGCCATATGGGCGATAATATGCGGCATGAAATGCAAGCGAACTTCCGACGGTCGCGCCCACGATCATCACACCTTGCAGGT
GATGCGGCAGCAGGCCATCAAAGCGGTGCGTGAGGGGCAGACCGTGCAGAGCGTCGCCGCCTCGTTCGGCGTGAATCCTCGCAGCGTCTTCCGCTGGCTG
GCCGACTTCGCCAACGGCGGCCAGAACGCCCTGCTCGCCAAGCCGATCCCGGGACGTCCGCCCAGGCTCAGCGCCGAGGAGATGGCCTGGCTCGCCCGCG
CGGTCCGCGACCACACCCCGCAGCAGTTCAAGTTCGAGTTCGGGCTGTGGACGCTGACGCTGATCCGTCACCTGATCAATCGTCAGCTCAAGAAGGAACT
GTCGGTTTCCTCGGTCCACCGGCTCATGAAGATCCTGGGCTTCAGCGCCCAGAAACCGCTCTATCAGGCCTGGCAGCAGGATCCCGTGCTGGTGCGGACC
TGGGAGACCGAGACCTATCCCGCCATTCGCGCCGAAGCGCGTGCGACGGGCGCGACGATCTACTTTGCCGACGAGTCGGGAATCCGTTCCGACTACCACA
CCGGCACCCCCTGGGCGCCGCAGGGCCAGACGCCGGTGGTGACGGCCACCGGCCGGCGCTTTTCGCTGAACATGATCTCGGCGGTGAGCGCGCAGGGCGA
GTTTCGGTTCATGCTGCATGAAGGCTCGGTCGGTGCGAAGGTCTTTCTGGAGTTCCTCAAGCGCTTGATGATCGGCGCTCAGAAGCCGGTGTTCGTCATC
GTCGATGGTCATCCGATCCACAAGGCAAAGTTGATCAAGACCTATGTCGACAGCCTCGGCGGCCGGCTGAAGCTGTTCTACCTGCCGCCGTATTCGCCGC
ACCTGAACCCGGACGAAACGGTCTGGGCGCAGGTGAAGCGCAAGGTTTCGCGCCAGGTGGTCGAGAGCAAGGAGGACATGAAGCGCCTCGCACTCGGGGC
CCTGCGCAGCATTCAGAAACTCCCCGAGCTCGTGAAGTCATTCTTCCGGCAACCAGAGTGTCAATACGCTCTATAGTGACACTACTTATTGAAAAGTTAG
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1032 bp343 aa451076+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MKCKRTSDGRAHDHHTLQVMRQQAIKAVREGQTVQSVAASFGVNPRSVFRWLADFANGGQNALLAKPIPGRPPRLSAEEMAWLARAVRDHTPQQFKFEFG
LWTLTLIRHLINRQLKKELSVSSVHRLMKILGFSAQKPLYQAWQQDPVLVRTWETETYPAIRAEARATGATIYFADESGIRSDYHTGTPWAPQGQTPVVT
ATGRRFSLNMISAVSAQGEFRFMLHEGSVGAKVFLEFLKRLMIGAQKPVFVIVDGHPIHKAKLIKTYVDSLGGRLKLFYLPPYSPHLNPDETVWAQVKRK
VSRQVVESKEDMKRLALGALRSIQKLPELVKSFFRQPECQYAL

 

Blast result :
Comments
ISAzo33 is 89% aa similar to ISAzo32.
References
1] Kuhner,S., Wohlbrand,L., Fritz,I., Wruck,W., Hultschig,C., Hufnagel,P., Kube,M., Reinhardt,R. and Rabus,R. (2005) J. Bacteriol. 187 (4), 1493-1503.

2] Rabus,R., Kube,M., Heider,J., Beck,A., Heitmann,K., Widdel,F. and Reinhardt,R. (2005) Arch. Microbiol. 183 (1), 27-36.

3] PROSCIENCE (2004) Direct Submission GenBank.

4] ISfinder annotation (2008).