ISSpn6
- Family IS200/IS605
- Group IS200
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_003028 | ND | Streptococcus pneumoniae | Streptococcus pneumoniae TIGR4 |
DNA section
IS Length : 736 bp
Ends
Left end : TGTCTCCCGAAAACCACTAGCTATGCTAGTGGTTCCATAGAGCTTTTAGCGTGGTAACAAAAAGAACCTCCTAAAATGATAAGATAGAAGTGGTTTCTCC II struct. : Yes
Right end : TGGTGCGCGAGGAAGCTATTTCGGTGGGCCTTTGGCCCTGGCCGGTAGAAGCGGCTTATAGCCGCAGAACAAACCACCAGTTCACACTGGTGGTTTTGAT II struct. : Yes
Insertion site
| Left flank | LE cleavage site | Right flank | RE cleavage site |
|---|---|---|---|
| ATCCAACTTTT | TTGT | TTAAAAAACTTGATACAT | TGAT |
| TGCAGTCTTTC | TTGT | TTATTTTTTCGCTTGGTA | tgat |
| ATCCAACTTTT | TTGT | TTAAAAAACTTGATACAT | tgat |
| ATCCAACTTTT | TTGT | TAAAAAACTTGATA | tgat |
| ATCCAACTTTT | TTGT | TAAAAAACTTGATACATA | TGAT |
| TATGCGGGCTT | TTTT | GTTAGCCTATCTTA | TGAT |
| TTACCCATCTT | TTTT | AAAAGGAGTTAAATATGA | TGAT |
| TTACCCATCTT | TTTT | AAAAGGAGTTAAATATGAT | TGAT |
| CTGTTGCTTTT | TTGT | AAAAGGAGTTAAATATG | TGAT |
DNA sequence
TGTCTCCCGAAAACCACTAGCTATGCTAGTGGTTCCATAGAGCTTTTAGCGTGGTAACAAAAAGAACCTCCTAAAATGATAAGATAGAAGTGGTTTCTCC
GCCACTACAACATATCATACAGGAGGTACCTCATGAGAGAGGATAATCAAAGTTTATCACATACCACATGGAATTGTAAATATCATATTGTTTTTGCACC
CAAATATCGTCGTCAAATCATTTATGGCAGATACAAAGCTAGTATCGGAAGAATCATACGTGACTTATGTGAGCGTAAGGGTGTAATAATCCATGAAGCG
AATGCTTGTTCAGACCATATTCACATGCTTATCAGTATTCCTCCGAAACTTAGTGTTTCGTCCTTTATGGGCTATTTAAAGGGCAAGAGCAGTTTGATGA
TTTTTGATAAGCATGCGAATTTAAAATACAAATATGGCAATCGCAAGTTTTGGTGTAGAGGCTATTATGTAGATACGGTAGGCCGTAATCAGAAAGTGAT
AGCTGAATATATTCAGAATCAATTACAAGAAGACAGAGTAGCAGACCAGCTCACGTTATTCGAGTCAGTAGATCCGTTTACTGGCGAAATAAATAAGAGG
AAGTAACTAAGGTGCTTTAGCACCTGCTCGGGAAAGTGGTGCGCGAGGAAGCTATTTCGGTGGGCCTTTGGCCCTGGCCGGTAGAAGCGGCTTATAGCCG
CAGAACAAACCACCAGTTCACACTGGTGGTTTTGAT
GCCACTACAACATATCATACAGGAGGTACCTCATGAGAGAGGATAATCAAAGTTTATCACATACCACATGGAATTGTAAATATCATATTGTTTTTGCACC
CAAATATCGTCGTCAAATCATTTATGGCAGATACAAAGCTAGTATCGGAAGAATCATACGTGACTTATGTGAGCGTAAGGGTGTAATAATCCATGAAGCG
AATGCTTGTTCAGACCATATTCACATGCTTATCAGTATTCCTCCGAAACTTAGTGTTTCGTCCTTTATGGGCTATTTAAAGGGCAAGAGCAGTTTGATGA
TTTTTGATAAGCATGCGAATTTAAAATACAAATATGGCAATCGCAAGTTTTGGTGTAGAGGCTATTATGTAGATACGGTAGGCCGTAATCAGAAAGTGAT
AGCTGAATATATTCAGAATCAATTACAAGAAGACAGAGTAGCAGACCAGCTCACGTTATTCGAGTCAGTAGATCCGTTTACTGGCGAAATAAATAAGAGG
AAGTAACTAAGGTGCTTTAGCACCTGCTCGGGAAAGTGGTGCGCGAGGAAGCTATTTCGGTGGGCCTTTGGCCCTGGCCGGTAGAAGCGGCTTATAGCCG
CAGAACAAACCACCAGTTCACACTGGTGGTTTTGAT
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 474 bp | 157 aa | 133 | 606 | + | No |
Chemistry : Y1
ORF sequence :
MREDNQSLSHTTWNCKYHIVFAPKYRRQIIYGRYKASIGRIIRDLCERKGVIIHEANACSDHIHMLISIPPKLSVSSFMGYLKGKSSLMIFDKHANLKYK
YGNRKFWCRGYYVDTVGRNQKVIAEYIQNQLQEDRVADQLTLFESVDPFTGEINKRK
YGNRKFWCRGYYVDTVGRNQKVIAEYIQNQLQEDRVADQLTLFESVDPFTGEINKRK
Blast result :
Comments
ISSpn6 is 91% aa similar to IS657.
References
1] ISfinder annotation (2008)
2] Tettelin,H., Nelson,K.E., Paulsen,I.T., Eisen,J.A., Read,T.D., Peterson,S., Heidelberg,J., DeBoy,R.T., Haft,D.H., Dodson,R.J., Durkin,A.S., Gwinn,M., Kolonay,J.F., Nelson,W.C., Peterson,J.D., Umayam,L.A., White,O., Salzberg,S.L., Lewis,M.R., Radune,D., Holtzapple,E., Khouri,H., Wolf,A.M., Utterback,T.R., Hansen,C.L., McDonald,L.A., Feldblyum,T.V., Angiuoli,S., Dickinson,T., Hickey,E.K., Holt,I.E., Loftus,B.J., Yang,F., Smith,H.O., Venter,J.C., Dougherty,B.A., Morrison,D.A., Hollingshead,S.K. and Fraser,C.M. (2001) Science 293 (5529), 498-506
2] Tettelin,H., Nelson,K.E., Paulsen,I.T., Eisen,J.A., Read,T.D., Peterson,S., Heidelberg,J., DeBoy,R.T., Haft,D.H., Dodson,R.J., Durkin,A.S., Gwinn,M., Kolonay,J.F., Nelson,W.C., Peterson,J.D., Umayam,L.A., White,O., Salzberg,S.L., Lewis,M.R., Radune,D., Holtzapple,E., Khouri,H., Wolf,A.M., Utterback,T.R., Hansen,C.L., McDonald,L.A., Feldblyum,T.V., Angiuoli,S., Dickinson,T., Hickey,E.K., Holt,I.E., Loftus,B.J., Yang,F., Smith,H.O., Venter,J.C., Dougherty,B.A., Morrison,D.A., Hollingshead,S.K. and Fraser,C.M. (2001) Science 293 (5529), 498-506