ISAzo31

  • Family IS5
  • Group IS5
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_006513 ND Azoarcus sp.
Aromatoleum aromaticum EbN1
Azoarcus sp. EbN1
DNA section
IS Length : 1324 bp

Ends


IR Length : 19/25

IRL : CAGGGTGATGCAAAAGCCAGTGAACGATTCGACGATGTCTTTGTCATAGA
IRR : CAGGCTGATGCAAAAACACTCGACACCGCGCCACCCGGGGCCGTCCAATT

Insertion site


Left flankDirect repeatRight flankDR Length
GCCATCGAGGTTAGTGCTGAAGTC4

DNA sequence

CAGGGTGATGCAAAAGCCAGTGAACGATTCGACGATGTCTTTGTCATAGAAACATTCCCAAGTCCATTGAGCTACGACGATGCGCGGCCAACTCGACCCC
CAGTCTTCGACGTTCCACTATTTCTCGCCCGAGTCGCGAGTCCCGGCCGACCATCCACTGCGCCGGGTGAAGAAACTGGCCGACCGCGCGCTCAGTGCGA
TATCGGCAGACCTGAACGGGCTTTACTCGAACATCGGTCGGCCGTCGATTGCACCGGAGCGTTTGCTCAAGGGGCAATTGCTGATCGCGCTGTACTCCAT
TCGCTCTGACCGTCAGTTCTGCGAGCAGCTCGATTACAACATCCTCTTTCGCTGGTTTCTTGACATGAATCTCGAGAGTACCAGCCTGGATCAGTCGAAC
TTCAGCCGACTGCGCGAGCGCCTGGTGCAGACCGACATCGCCCGGCGCTTCTTCGACGAGGTGGTGAGCCTGGCCCGGCGCGAGAAATTGCTCTCGTCCG
ATCACTTCACCGTCGACGGCACGCTGATCGACGCATGGGCCTCGTTCAAAAGCTTCAAGCGCAAAGATGGTGAGCCGCCCAAGGACGGCGGCGATGGCAC
CGGAATGGTCGATTTCAAGGGCGAGAAGCGCTCGAACGCCACCCATCAGAGCACCACCGACCCCGAATCCCGGCTCATGAGGAAGGGCAATGGACAGCCC
GCCAAGCTCAGCTATGGAGGTCACGTGCTCATGGAGAATCGGAACGGGCTGTGCGTCGACATCCTGATCACCGAATCCACCCAGGCCGAACATCGCGCTG
CGCGCCAACTGCTCACCCGTGCTCGGCGCCGGCGTATTCATCCGAAGACCTTGGGCGCCGACAAGGGCTATCACGTCAAGGAGTTCGTCAGCCATCTTCG
TGAGCATCGTGTCCGCCCGCACATTGCACGGATCGCGAACCGAACGACACCGGGGCTCGACGGACGGACGACGAGAACCGAGGGCTACCAAATCAGTCAG
CGCAAGCGAAAACGGGTCGAGGAAATCTTTGGCTGGTTGAAGACGGTTGGCGGAATGCGCAAGACCCGATTTATCGGCCAGGAAAAAACCCAGATGGCTG
CATTTATTTCGGGGGCCGCCTACAACCTGTTGCGAATCGCGAAGTTGAGCGGTTCGGAGGTGAAGGCGTGAACCGGAGCGCCAAAATGGCCCGCGCAGGG
CACGAGAAACACCCTTTCGGCAATTCGAAAGGGATTGAAAATCCAATTCCTGAGACGAAGTCGGCATGAAATTCAATTGGACGGCCCCGGGTGGCGCGGT
GTCGAGTGTTTTTGCATCAGCCTG
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1092 bp363 aa801171+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MRGQLDPQSSTFHYFSPESRVPADHPLRRVKKLADRALSAISADLNGLYSNIGRPSIAPERLLKGQLLIALYSIRSDRQFCEQLDYNILFRWFLDMNLES
TSLDQSNFSRLRERLVQTDIARRFFDEVVSLARREKLLSSDHFTVDGTLIDAWASFKSFKRKDGEPPKDGGDGTGMVDFKGEKRSNATHQSTTDPESRLM
RKGNGQPAKLSYGGHVLMENRNGLCVDILITESTQAEHRAARQLLTRARRRRIHPKTLGADKGYHVKEFVSHLREHRVRPHIARIANRTTPGLDGRTTRT
EGYQISQRKRKRVEEIFGWLKTVGGMRKTRFIGQEKTQMAAFISGAAYNLLRIAKLSGSEVKA

 

Blast result :
Comments
ISAzo31 is 65% aa similar to ISGsu2.
References
1] Kuhner,S., Wohlbrand,L., Fritz,I., Wruck,W., Hultschig,C., Hufnagel,P., Kube,M., Reinhardt,R. and Rabus,R. (2005) J. Bacteriol. 187 (4), 1493-1503.

2] Rabus,R., Kube,M., Heider,J., Beck,A., Heitmann,K., Widdel,F. and Reinhardt,R. (2005) Arch. Microbiol. 183 (1), 27-36.

3] PROSCIENCE (2004) Direct Submission GenBank.

4] ISfinder annotation (2008).