ISPlr1
- Family ISAs1
- Group
Isoform Synonym(s) mcyDIS2, mcyDIS1, mcyEGIS, mcyAIS
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| AM039947 | ND | Planktothrix rubescens | Planktothrix rubescens No110 Planktothrix rubescens No178 Planktothrix rubescens No139 Planktothrix rubescens No145 Planktothrix rubescens No161 Planktothrix rubescens No166 Planktothrix rubescens No169 Planktothrix rubescens No170 |
DNA section
IS Length : 1423 bp
Ends
IR Length : 13
IRL : CAGGGCTGTTTCAAAGTCAGGTGAAAAAGAGTAAAGAAAGAGCGATCGCC
IRR : CAGGGCTGTTTCATGTTTGAAAATTATAGCCTACTTTTGGGTCATAATTC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TTCTGCCCCC | CCCGTGCACG | GGGGGTTTGG | 10 |
| CTGCCCCCCC | CGTGCACGGG | GGGTTTGGGG | 10 |
| GTTTGGGGGG | GGCTGTTCCC | TCTCTCAACT | 10 |
| TCAACTACCA | CCCAAAACCC | TAGCCTTCCG | 10 |
DNA sequence
CAGGGCTGTTTCAAAGTCAGGTGAAAAAGAGTAAAGAAAGAGCGATCGCCACCTGATAAAATGGAGAGCGATCACCATTTTTTTCTAAAATAAAGATGCT
AACAAGTTTAATAGAAAATTTAAAAGAAGTCAAGGATTTCCGAAAAAATCAGGGAAAAAGGTATAGTTTATGGGAAGTGCTATTAGTAGTAGTTTTAGGG
GTGATGTCAGGGCATCAAGGATATCGAGAGATGGAATATTTTGTCAAAGCCAACGAAGTTATCCTGAAAAGAACATTCAATATCTATAGCCAAGGAATGC
CATCTTATTCAACAATAAGGAGGGTGATGAGAGGAGTAGACGAGAAGGATTTAAGCAAAATAGTAAAAGAATGGTCAAGAGAGAATTCTCCTAAATTAAA
GGGAATAGAGGGATTAGCAATAGATGGAAAAAGTTTAAGAAGTACGGTAAAAGACCCAGGCAATCAGCAGCAAAACATGGTAATAATGGTATCTCTATTT
AGTCAAGAAACCGGATTAGTATTAGCGACGGAAAAGTTCGAGAGTAAAACCGGTTCAGAACAAGCTGTAGCCCAAGGGATAATAGAAGAATGTGGTCTAA
AAGGAAAGTTAATAACTGCGGATGCGTTACATTGTAATGTCACCCCAGTCCGAAAGATTATCGAGAGTGAAAACGATTATTTAATCGCGGTTAAAAAGAA
TCAAATCAAACTGTATAATCAAATTGAAAAAATTACAAAAACGACGAAAGCTGAAAGTATAGATACTCAAAAAGAGAGGGGTCATGGACGAGAAATAACT
CGTCGGGTATCAGTGTTTAAAAATTCGATAAAAATTGAAAGCCCTTGGGAACATATTCAAAGTATAATTCAGGTAGAAAGGTGGGGATACAGAGGAAAAA
AGCCTTATAGAGAAACGGTTTATTATATAAGTAGTATCTGGGAAAAGGCGGATTTTTTTTCCAAAAAAATTAAAGGGCATTGGGGGATTGAGAATCAAGT
TCATTGGGTAAAAGATGTGTTATTTAAAGAGGATAGTATGAAAATTCATCAAGTACAAGCCGCGACTAATTGGGCACTGCTCAACACCCTAGGATTGAAT
ATTTTCAGAGGTTTAGGATTTTTGTCAATAACAGAGGGAAGAAGGTGGTTAGGAAATCATTGGGAGAAATTAGTGGCTATCTCTTAATCCGAAAGTTAAA
AATGAAAAAATAGAGCAATTAATTTAGGAAAAATAGATAAAAATCGGGAATGTAAGTCTTACTCCATAGGGAATAAGAATATTTATGCTGCTTAGATAAT
ACCTCCTATAATTAATATTAATATAGCAATTATATGAAATATTAATTATTCGGAAAATAGCTATCATGTTTGAGAATTATGACCCAAAAGTAGGCTATAA
TTTTCAAACATGAAACAGCCCTG
AACAAGTTTAATAGAAAATTTAAAAGAAGTCAAGGATTTCCGAAAAAATCAGGGAAAAAGGTATAGTTTATGGGAAGTGCTATTAGTAGTAGTTTTAGGG
GTGATGTCAGGGCATCAAGGATATCGAGAGATGGAATATTTTGTCAAAGCCAACGAAGTTATCCTGAAAAGAACATTCAATATCTATAGCCAAGGAATGC
CATCTTATTCAACAATAAGGAGGGTGATGAGAGGAGTAGACGAGAAGGATTTAAGCAAAATAGTAAAAGAATGGTCAAGAGAGAATTCTCCTAAATTAAA
GGGAATAGAGGGATTAGCAATAGATGGAAAAAGTTTAAGAAGTACGGTAAAAGACCCAGGCAATCAGCAGCAAAACATGGTAATAATGGTATCTCTATTT
AGTCAAGAAACCGGATTAGTATTAGCGACGGAAAAGTTCGAGAGTAAAACCGGTTCAGAACAAGCTGTAGCCCAAGGGATAATAGAAGAATGTGGTCTAA
AAGGAAAGTTAATAACTGCGGATGCGTTACATTGTAATGTCACCCCAGTCCGAAAGATTATCGAGAGTGAAAACGATTATTTAATCGCGGTTAAAAAGAA
TCAAATCAAACTGTATAATCAAATTGAAAAAATTACAAAAACGACGAAAGCTGAAAGTATAGATACTCAAAAAGAGAGGGGTCATGGACGAGAAATAACT
CGTCGGGTATCAGTGTTTAAAAATTCGATAAAAATTGAAAGCCCTTGGGAACATATTCAAAGTATAATTCAGGTAGAAAGGTGGGGATACAGAGGAAAAA
AGCCTTATAGAGAAACGGTTTATTATATAAGTAGTATCTGGGAAAAGGCGGATTTTTTTTCCAAAAAAATTAAAGGGCATTGGGGGATTGAGAATCAAGT
TCATTGGGTAAAAGATGTGTTATTTAAAGAGGATAGTATGAAAATTCATCAAGTACAAGCCGCGACTAATTGGGCACTGCTCAACACCCTAGGATTGAAT
ATTTTCAGAGGTTTAGGATTTTTGTCAATAACAGAGGGAAGAAGGTGGTTAGGAAATCATTGGGAGAAATTAGTGGCTATCTCTTAATCCGAAAGTTAAA
AATGAAAAAATAGAGCAATTAATTTAGGAAAAATAGATAAAAATCGGGAATGTAAGTCTTACTCCATAGGGAATAAGAATATTTATGCTGCTTAGATAAT
ACCTCCTATAATTAATATTAATATAGCAATTATATGAAATATTAATTATTCGGAAAATAGCTATCATGTTTGAGAATTATGACCCAAAAGTAGGCTATAA
TTTTCAAACATGAAACAGCCCTG
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1092 bp | 363 aa | 96 | 1187 | + | No |
Chemistry : Unknow
ORF sequence :
MLTSLIENLKEVKDFRKNQGKRYSLWEVLLVVVLGVMSGHQGYREMEYFVKANEVILKRTFNIYSQGMPSYSTIRRVMRGVDEKDLSKIVKEWSRENSPK
LKGIEGLAIDGKSLRSTVKDPGNQQQNMVIMVSLFSQETGLVLATEKFESKTGSEQAVAQGIIEECGLKGKLITADALHCNVTPVRKIIESENDYLIAVK
KNQIKLYNQIEKITKTTKAESIDTQKERGHGREITRRVSVFKNSIKIESPWEHIQSIIQVERWGYRGKKPYRETVYYISSIWEKADFFSKKIKGHWGIEN
QVHWVKDVLFKEDSMKIHQVQAATNWALLNTLGLNIFRGLGFLSITEGRRWLGNHWEKLVAIS
LKGIEGLAIDGKSLRSTVKDPGNQQQNMVIMVSLFSQETGLVLATEKFESKTGSEQAVAQGIIEECGLKGKLITADALHCNVTPVRKIIESENDYLIAVK
KNQIKLYNQIEKITKTTKAESIDTQKERGHGREITRRVSVFKNSIKIESPWEHIQSIIQVERWGYRGKKPYRETVYYISSIWEKADFFSKKIKGHWGIEN
QVHWVKDVLFKEDSMKIHQVQAATNWALLNTLGLNIFRGLGFLSITEGRRWLGNHWEKLVAIS
Blast result :
Comments
ISPlr1 is 51% aa similar to ISPto5.
McyDIS2 isolated from Planktothrix rubescens strain No178 was inserted into the microcystin (mcy) gene cluster leading to the inactivation of microcystin synthesis in Planktothrix spp.(pos. 11,918, AJ441056).
Planktothrix rubescens strains No139, 145, 161, 166, 169, 170 (AM039941-AM039947) contained almost identical IS elements (99.5-99.9% similarity) that were inserted at the same position of the mcy gene cluster (pos. 11,918, AJ441056).
Planktothrix rubescens strain No110 (mcyDIS1, AM039940) contained an almost identical IS element (99.3% similarity) that was inserted at the same position of the mcy gene cluster (pos. 11,918, AJ441056), however, mcyDIS1 was transcribed in the opposite direction.
Planktothrix rubescens strain No110 (mcyEGIS, AM055629) contained another almost identical IS element (99.9% similarity) that was inserted into the intergenic spacer region between mcyE and mcyG (pos. 23,822, AJ441056).
Planktothrix rubescens strain No40 (mcyAIS, AM039939) contained another almost identical IS element (99.0% similarity) that was inserted into the mcyA gene (pos. 41,274, AJ441056).
McyDIS2 isolated from Planktothrix rubescens strain No178 was inserted into the microcystin (mcy) gene cluster leading to the inactivation of microcystin synthesis in Planktothrix spp.(pos. 11,918, AJ441056).
Planktothrix rubescens strains No139, 145, 161, 166, 169, 170 (AM039941-AM039947) contained almost identical IS elements (99.5-99.9% similarity) that were inserted at the same position of the mcy gene cluster (pos. 11,918, AJ441056).
Planktothrix rubescens strain No110 (mcyDIS1, AM039940) contained an almost identical IS element (99.3% similarity) that was inserted at the same position of the mcy gene cluster (pos. 11,918, AJ441056), however, mcyDIS1 was transcribed in the opposite direction.
Planktothrix rubescens strain No110 (mcyEGIS, AM055629) contained another almost identical IS element (99.9% similarity) that was inserted into the intergenic spacer region between mcyE and mcyG (pos. 23,822, AJ441056).
Planktothrix rubescens strain No40 (mcyAIS, AM039939) contained another almost identical IS element (99.0% similarity) that was inserted into the mcyA gene (pos. 41,274, AJ441056).
References