ISAzo30
- Family IS630
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_006513 | ND | Azoarcus sp. | Aromatoleum aromaticum EbN1 Azoarcus sp. EbN1 |
DNA section
IS Length : 1100 bp
Ends
IR Length : 19/26
IRL : CTCAGAAGGCGGGAAGTAAGGCGACATGATGGGGAACTTTCGCCGTCCTG
IRR : ATCATGAGGCGGAAATATAGGCGACACTGGGATGCTTGAAGAAAGAGCGC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GCCGGTTGTT | TA | AAACCACGAC | 2 |
DNA sequence
CTCAGAAGGCGGGAAGTAAGGCGACATGATGGGGAACTTTCGCCGTCCTGGCGATCCGATGAGGATGAAACGAGACGGACGAAAACTGGATCACGGGACG
CTGGAGACGATTCGTAGGATGGCCATTGAGCGGGTGCGGGAAGGCGAAGCGCCGAGCCAGGTGATCGCCAGCTACGGATTTCATCGCAGCGTGATCTATC
GCTGGCTGAAAGCGGCGGCGGGCCGCGGCCATGGGCTGCGGGCGCTGGCGTCGCATCCGATCACTGGACGGCCCCCGAAACTCACCGCGCAGCAGGCGCG
GCAGGTATTGCGCTGGATCGACGGGCACTCGCCGATGGAACATGGCTTCGACACCGGGCTGTGGACACGGGGTCTGGTGCGGGACTTGGTGCAGAGCAAA
TTTGGCATCACCCTGAGCCTGTCCTCGATTGGTGCGCTGCTGGCGCGACTGGGCCTGACGGTGCAAAAGCCGCTGCAGCGCGCTTACCAGCGTGATCCGG
CGGCGATTGAGCGCTGGCAGCACGAGACCTATCCGGCGCTGATGCAGCGTGCCAGGGAAGAGAAAGCCGAGATTCTGTTTTGGGACGAATCCGGCTTTCG
CGCCGACGCCGTGCAGGGCAGGACTTGGGGCGCCAAGGGTCGCACACCGGTGGTCGCGGTGCCCGGGCAGCGGCAAAGTATCAGTGCGGCCTCGGCCGTC
AGCATGAAGGGCGGCTTCTGGTTCGCCATCTACCCTGGGGCATTGAACGGCGAACTGTTTGTGGACTTGCTCAGGAAGCTGATGCACCGCCGCAGGAAGC
CCTTGCACCTGGTGCTCGACGGACTGCCCGCGCACAAGAAGGCGGGGGTCAAGACGTATGTCGCCAGCACGCAAGGCAAGCTGACGCTGCATTTCCTGCC
GGGCTATGCCCCCGACCTGAATCCGGATGAACTGGTGTGGAGCCACGCCAAGCGTACCGGCAACGCCCGCCGGCCCCTGCAAAAAGGCGAGACCCTGCAA
GAACGTGTGCATGACCAGTTGGCCGATATCGGCAAGCATCCCGCCTTGGTGCGCTCTTTCTTCAAGCATCCCAGTGTCGCCTATATTTCCGCCTCATGAT
CTGGAGACGATTCGTAGGATGGCCATTGAGCGGGTGCGGGAAGGCGAAGCGCCGAGCCAGGTGATCGCCAGCTACGGATTTCATCGCAGCGTGATCTATC
GCTGGCTGAAAGCGGCGGCGGGCCGCGGCCATGGGCTGCGGGCGCTGGCGTCGCATCCGATCACTGGACGGCCCCCGAAACTCACCGCGCAGCAGGCGCG
GCAGGTATTGCGCTGGATCGACGGGCACTCGCCGATGGAACATGGCTTCGACACCGGGCTGTGGACACGGGGTCTGGTGCGGGACTTGGTGCAGAGCAAA
TTTGGCATCACCCTGAGCCTGTCCTCGATTGGTGCGCTGCTGGCGCGACTGGGCCTGACGGTGCAAAAGCCGCTGCAGCGCGCTTACCAGCGTGATCCGG
CGGCGATTGAGCGCTGGCAGCACGAGACCTATCCGGCGCTGATGCAGCGTGCCAGGGAAGAGAAAGCCGAGATTCTGTTTTGGGACGAATCCGGCTTTCG
CGCCGACGCCGTGCAGGGCAGGACTTGGGGCGCCAAGGGTCGCACACCGGTGGTCGCGGTGCCCGGGCAGCGGCAAAGTATCAGTGCGGCCTCGGCCGTC
AGCATGAAGGGCGGCTTCTGGTTCGCCATCTACCCTGGGGCATTGAACGGCGAACTGTTTGTGGACTTGCTCAGGAAGCTGATGCACCGCCGCAGGAAGC
CCTTGCACCTGGTGCTCGACGGACTGCCCGCGCACAAGAAGGCGGGGGTCAAGACGTATGTCGCCAGCACGCAAGGCAAGCTGACGCTGCATTTCCTGCC
GGGCTATGCCCCCGACCTGAATCCGGATGAACTGGTGTGGAGCCACGCCAAGCGTACCGGCAACGCCCGCCGGCCCCTGCAAAAAGGCGAGACCCTGCAA
GAACGTGTGCATGACCAGTTGGCCGATATCGGCAAGCATCCCGCCTTGGTGCGCTCTTTCTTCAAGCATCCCAGTGTCGCCTATATTTCCGCCTCATGAT
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1074 bp | 357 aa | 26 | 1099 | + | No |
Chemistry : DDE
ORF sequence :
MMGNFRRPGDPMRMKRDGRKLDHGTLETIRRMAIERVREGEAPSQVIASYGFHRSVIYRWLKAAAGRGHGLRALASHPITGRPPKLTAQQARQVLRWIDG
HSPMEHGFDTGLWTRGLVRDLVQSKFGITLSLSSIGALLARLGLTVQKPLQRAYQRDPAAIERWQHETYPALMQRAREEKAEILFWDESGFRADAVQGRT
WGAKGRTPVVAVPGQRQSISAASAVSMKGGFWFAIYPGALNGELFVDLLRKLMHRRRKPLHLVLDGLPAHKKAGVKTYVASTQGKLTLHFLPGYAPDLNP
DELVWSHAKRTGNARRPLQKGETLQERVHDQLADIGKHPALVRSFFKHPSVAYISAS
HSPMEHGFDTGLWTRGLVRDLVQSKFGITLSLSSIGALLARLGLTVQKPLQRAYQRDPAAIERWQHETYPALMQRAREEKAEILFWDESGFRADAVQGRT
WGAKGRTPVVAVPGQRQSISAASAVSMKGGFWFAIYPGALNGELFVDLLRKLMHRRRKPLHLVLDGLPAHKKAGVKTYVASTQGKLTLHFLPGYAPDLNP
DELVWSHAKRTGNARRPLQKGETLQERVHDQLADIGKHPALVRSFFKHPSVAYISAS
Blast result :
Comments
ISAzo30 is 85% aa similar to ISCARN31.
References
1] Kuhner,S., Wohlbrand,L., Fritz,I., Wruck,W., Hultschig,C., Hufnagel,P., Kube,M., Reinhardt,R. and Rabus,R. (2005) J. Bacteriol. 187 (4), 1493-1503.
2] Rabus,R., Kube,M., Heider,J., Beck,A., Heitmann,K., Widdel,F. and Reinhardt,R. (2005) Arch. Microbiol. 183 (1), 27-36.
3] PROSCIENCE (2004) Direct Submission GenBank.
4] ISfinder annotation (2008).
2] Rabus,R., Kube,M., Heider,J., Beck,A., Heitmann,K., Widdel,F. and Reinhardt,R. (2005) Arch. Microbiol. 183 (1), 27-36.
3] PROSCIENCE (2004) Direct Submission GenBank.
4] ISfinder annotation (2008).